Amsbio reports how the Sasaki Lab within the School of Veterinary Medicine at the University of Pennsylvania (USA) devised a protocol in which induced pluripotent stem cells (iPSCs) could be utilized to produce reconstituted human testes. Since the original 2020 publication of their groundbreaking research, Dr Kotaro Sasaki’s team has made significant progress in reconstituting human testis-like tissue from iPSCs...

Amsbio has launched a new range of Deubiquitinating (DUB) enzyme assay kits supplied in a convenient and easy-to-use 96-well assay format. With minimal setup required and no need for substrate preparation, these ready-to-use kits accelerate routine DUB activity assays without compromising data quality. Ubiquitination and deubiquitination are two important physiological processes in the ubiquitin-proteasome system, responsible for protein degradation in cells...

AMSBIO announces a new range of ready-to-use Human induced Pluripotent Stem Cell (hiPSC) -derived cardiomyocytes, hepatocytes, and neurons. Supplied fully differentiated and available in high-purity formats, these ready-to-use cells help accelerate research by streamlining lab workflows, eliminating the need for in-house differentiation and minimizing preparation time...

AMSBIO has implemented a program of investment to support the students at Penn State Lehigh Valley, part of Pennsylvania State University, in their transformative Course-based Undergraduate Research Experience (CURE) program. The CURE program, led by Dr. Jacqueline McLaughlin, allows students to dive into real-world research projects that tackle critical challenges in health and medicine...

EZ-Spread™ Plating Beads from AMSBIO allow you to spread bacterial and yeast cells across the surface of an agar plate in just seconds. Eliminating the traditional need for glass rods and potentially hazardous ethanol flaming - EZ-Spread plating beads provide a safer and more convenient method for spreading bacterial and yeast cells on agar plates. Manufactured using precision molds, resulting in uniform shape and diameter, EZ-Spread plating beads ensure more even spreading of cells leading to...

AMSBIO announce a new range of Synthetic Nanodiscs that enable preparation of multi-pass transmembrane proteins in their active form, revolutionizing the future of membrane protein research and drug development. Membrane Proteins account for more than 60% of all FDA-approved drug targets and 90% of antibody-based drug targets. Many traditional sample preparation methods struggle with challenges in obtaining water-soluble and bioactive forms of multi-pass transmembrane proteins...

AMSBIO has launched CellO-IF – an all-in-one immunofluorescent staining reagent kit designed to accelerate the labelling organoids and spheroids directly in hydrogels or extracellular matrices, while preserving delicate structures and cellular integrity. Immunofluorescence (IF) allows detection and localization of antigens in diverse types of tissues in various cell preparations. The technique provides excellent sensitivity and amplification of signal by comparison to immunohistochemistry...

BioPORTER^® protein delivery reagent from AMSBIO is proven to efficiently transport functionally active proteins directly into living cells eliminating the traditional DNA transfection, transcription, and protein translation process. Employing a unique lipid-based protein delivery system – BioPORTER® makes studying protein functionality faster and easier than ever before...

The MycoScope™ Mycoplasma PCR detection kit from AMSBIO uses polymerase chain reaction (PCR) technology to detect mycoplasma infections in less than 3 hours. Offering high-sensitivity detection, rapid results, and compatibility with existing PCR setups, this kit reduces the risk of contamination spread saving valuable time and resources. As reported in the ISSCR standards report (2023) a sizeable proportion of cell cultures (up to >80%) are infected with mycoplasma...

AMSBIO has launched a range of genetically engineered Induced Pluripotent Stem Cells (iPSCs) developed to provide improved model fidelity over cell lines and biochemical assays used for drug discovery and cell therapy research. These iPSC cell lines offer improved efficiency and cost savings compared to animal models, enhancing high-throughput reporter lines, human disease models and toxicity screening.

AMSBIO offers a range of kits, media and reagents enabling development of food in a way that is not wasteful of our natural resources and is not detrimental to the environment or consumer health. Recent advances in biotechnological methods have enabled the development of cultured meat - meat grown in a laboratory using stem cell technology that mimics the texture, flavor, and nutritional value of conventional meat...

AMSBIO, a leading provider of innovative products and services for the life sciences community, announces the launch of its Expanded Stem Cell Synergy Solutions. Over the last two decades AMSBIO has established itself as a leading international supplier of solutions for streamlined and efficient ES/iPS Cell Culture using feeder-free StemFit® media and iMatrix recombinant laminin extracellular matrices...

CELLBANKER® freezing media from AMSBIO is now available in customizable formats, seamlessly integrating into cell manufacturing processes with personalized formulations, volumes, and packaging. Cited in over 500 scientific publications, the CELLBANKER range has proven itself as a safe and reliable solution for storing cells and tissues. This includes GMP-grade, chemically defined formulations specifically designed for the storage of biospecimens for clinical applications...

AMSBIO has launched a new 2024 travel grant award scheme for scientists under the age of thirty, employed in academia, research institutions, or companies engaged in cancer and stem cell research. Working closely with the global life science community for over three decades AMSBIO believes in partnership and paying back by supporting new research and researchers. Last year, the company funded a travel grant to attend ISSCR 2023...

AMSBIO has expanded its range of high-performance magnetic beads for use as detection labels in magnetic immunoassays or as a solid support phase in immunoassays. MagSi-STA beads are magnetic silica particles with high quality streptavidin covalently attached to the bead surface. Available in different mean sizes, streptavidin coupling chemistries and binding capacities, these industry-leading MagSi-STA beads offer an optimized solution for many in vitro diagnostic applications, including immunoassays.

In an informative new blog post - AMSBIO reflects on a selection of the ground breaking life science and biomedical research undertaken by its customers during 2023. From deciphering the intricacies of cancer progression mechanisms to revolutionising wound care, AMSBIO is delighted to have played a supporting role in these scientific endeavours. Discover an exciting collaboration led by Dr. Ashley M. Laughney from Weill Cornell Medicine and Dr. Samuel F. Bakhoum from Memorial Sloan Kettering Cancer Center...

The new ABLE 3D Magnetic Stir and Disposable Bioreactor System from AMSBIO provides cost-effective, lab-scale production of induced pluripotent stem (iPS) cell spheroid culture. Induced pluripotent stem cells grown in 3D spheroid suspension culture closely resemble embryoid body structures naturally formed by embryonic stem cells. Using an ABLE 3D Bioreactor offers researchers an easy-to-use and affordable tool that provides excellent yield, viability, and high efficiency for the expansion of human iPS stem cells and differentiation...

AMSBIO has published a case study that describes how a team of scientists, led by Dr. Roger Kamm from the Massachusetts Institute of Technology (MIT), has developed a protocol for the fabrication of a 3D microfluidic neuromuscular platform that can be used to model Motor Neuron Diseases (MND’s). Researchers at MIT have developed novel approaches to unravel the pathogenesis of MNDs using CELLBANKER® 1 and STEM-CELLBANKER® cryopreservation media from AMSBIO...

AMSBIO, a provider of cutting-edge products and services for accelerating stem cell research, has sponsored two aspiring life scientists to attend and present posters at the recent ISSCR 2023 conference in Boston, USA. Whilst the original award was for one researcher, AMSBIO doubled the fund and awarded two grants based on the high quality of the entries. Krystyna Joyce, AMSBIO’s marketing manager, said "The entries were so impressive that we ended up sponsoring two winners instead of one"...

AMSBIO, a leading supplier of human cell lines and research tools, is proud to announce its collaboration with WARR Space Labs, a student group from the Technical University of Munich (TUM). The students undertook an exciting project aimed at advancing our understanding of neurodegenerative disorders, particularly Alzheimer’s disease, through spaceborne experiments.

AMSBIO announce a new range of pre-made Nano-Lantern lentivirus products that utilize a proprietary lentiviral vector system to enable highly sensitive and precise in vivo luminescence imaging. This cutting-edge Nano-Lantern lentivirus technology, which consists of an enhanced Renilla-Luciferase connected to an Orange Fluorescent protein (OFP), represents a significant improvement over previous methods and is poised to revolutionize many in vivo imaging applications...

AMSBIO has published a blog about Dr. Satoshi Okamoto’s groundbreaking research on the production of induced pluripotent stem cells (iPSC)-derived liver organoids, and their significant potential in clinical research trials. The primary objective of Dr. Okamoto's research program at the Yokohama City University Graduate School's Department of Regenerative Medicine was to achieve large-scale, stable production of clinical-grade liver organoids...

AMSBIO announce MatriMix – a groundbreaking 3D culture substrate. This pioneering hydrogel sets a new benchmark in the field, containing fully defined components, including medical grade collagens, laminin-511 E8 fragments, and hyaluronic acid. The high lot-to-lot consistency of MatriMix enables researchers to obtain highly reproducible results. MatriMix overcomes the limitations of current 3D substrates, enabling cutting-edge applications...

A recent blog post from AMSBIO introduces a selection of the ‘green products’ it offers to life science researchers looking to maintain rigorous scientific standards in their lab whilst also trying to minimize environmental impact. Laboratories are known to consume a significant amount of energy and produce a considerable amount of waste. However, with the increasing awareness of the importance of sustainability, many life sciences labs are taking steps towards prioritizing sustainable products and practices...

AMSBIO is pleased to announce the launch of Extragel, a like-for-like replacement for Matrigel^TM, Geltrex^TM and Cultrex^TM Basement Membrane Extract (BME). Available ex-stock, with overnight shipment, Extragel is a reconstituted matrix hydrogel formed by basement membrane components extracted from mouse tumor tissues which are rich in extracellular matrix proteins. Designed to be used undiluted or diluted to a specific protein concentration...

AMSBIO has interviewed two extraordinary women in the 3D Cell Culture and Organoid field; Dr Hynda Kleinman, one of the co-inventors of Matrigel, and Dr Meritxell Huch, a director at the Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) to mark International Women’s Day on the 8th March, and World Organoid Research Day on 22nd March 2023...

The new MagSi-DNA FFPE kit from AMSBIO enables quick and easy isolation of genomic DNA (gDNA) from mammalian formalin-fixed paraffin-embedded (FFPE) tissue or cell samples. Developed using proprietary magnetic bead technology – the MagSi-DNA FFPE kit can be used for manual and automated isolation of gDNA from mammalian FFPE tissue or cell samples...

AMSBIO reports how the Faculty of Biological Sciences at the University of Leeds (UK) is using their specialty reagents for the production, visualization, and analysis of glycans and to investigate their interactions which are instrumental to the advance of glycobiology. Glycobiology is vital for many biological processes — from fertilization to inflammation — helping unlock information about interactions between carbohydrates and proteins...

AMSBIO announce a new range of different isolation products for fast, scalable, and reproducible purification of Extracellular Vesicles (EV), including exosomes, as well as addressing EV heterogeneity and high throughput solutions for biomarker discovery. Traditionally ultracentrifugation has been the favored method for EV isolation or separation. Though it is still widely used, ultracentrifugation not only tends to alter the vesicle shape and functionality but is time-consuming and requires expensive equipment...

AMSBIO offers a wide range of small molecules to provide scientists with targeted tools to direct cell fates, making them crucial in areas including regenerative medicine and cancer research. Small molecules are cell-permeable organic compounds with low molecular weights, which are chemically produced, in contrast to larger molecules such as proteins which are often produced via biological means...

AMSBIO has published an informative handbook for scientists who need to preserve or archive organoids in a research lab. The handbook brings together published paper citations, quotes and data from leading international research groups who are using the CELLBANKER® range of products to cryopreserve their organoids simply and safely. In addition, the handbook includes best practice protocols plus information making it easier to understand the process of freezing organoids...

AMSBIO has added a new range of Vesicular Stomatitis Virus (VSV) to its suite of virus-based tools for cell engineering and the study of viral infection. Vesicular Stomatitis Virus is an enveloped, negative-stranded RNA virus with a simple structure. Its ability to infect a wide range of mammalian cell types has made it a valuable tool to study virus entry, replication, and assembly. A key advantage of the AMSBIO VSV model is that it undergoes only one round of replication....

AMSBIO announce RNAdvantage – the first product in a new range of stabilization solutions that provide robust protection for your RNA samples at ambient temperature. Designed to protect and preserve RNA from most tissues, cultured cells, bacteria, and yeast for up to 14-days, RNAdvantage eliminates the traditional costs of flash freezing samples dry-ice shipping and freezer storage...

AMSBIO has published an interview with Professor Marius Wernig from Stanford University, Pathology Stem Cell Institute that discusses what could be the world’s first widely applicable curative treatment for Epidermolysis Bullosa (EB). This rare genetic disease causes chronic and incredibly painful skin wounds that often lead to an aggressive form of skin cancer and eventual death...

Lentiviral vectors are a valuable tool for delivering target genes into most types of dividing and non-dividing mammalian cells in vitro and in vivo. Typically, production of custom lentiviral vectors is a laborious and challenging process, especially when high-titer lentiviral vectors are required. The custom lentiviral vector production service from AMSBO offers researchers a proven partner to avoid this bottleneck and speed up your research...

A screening tool developed in research led at the University of Strathclyde, UK could increase the number of tests on a solid tumour sample by up to 50 times. The tool is designed for testing at scale the latest immunotherapies, such as Chimeric Antigen Receptor (CAR) T-cell therapy, which is effective against many haematological cancers but presents challenges when used to treat solid tumours. Traditional 2D models that are currently used fail to reproduce the complexity of the tumour’s microenvironment...

AMSBIO announce a new range of high-quality bispecific antibodies (BsAbs) and trispecific antibodies (TsAbs) that can used to provide enhanced cancer therapeutic efficacy and/or safety compared to what can be achieved with conventional monospecific antibodies. The concept of using a molecule with more than one binding site to enhance its biological function has been around for some time....

AMSBIO announces the launch of the QuicKey Pro™ range of ELISA kits – new easy-to-use assays that require just one step of washing, and one step of sample addition, resulting in a whole experiment taking only 90 minutes. Compared with traditional ELISA kits, the total procedure time using a QuicKey Pro™ ELISA kit is reduced by 1-2 hours, resulting in significant gains in experimental efficiency....

AMSBIO has introduced further essential tools for SARS-CoV-2 researchers to help decipher the COVID-19 B.1.1.529 variant. New to the AMSBIO range are recombinant proteins, easy-to-perform assay kits, Omicron pseudotyped lentiviruses and spike neutralizing antibodies for use in functional assays to block SARS-CoV-2 spike protein and ACE2 binding...

AMSBIO announce the addition of several new products to its growing range of magnetic bead based nucleic acid extraction kits for pathogens detection. The MagSi-NA Pathogens MSP (Multiple Sample Pooling) kit sets a new benchmark for automated isolation of nucleic acids (RNA and DNA) from respiratory samples. Sample pooling is useful in times of high testing and low infection prevalence where traditional sample screening methods are not cost-effective....

AMSBIO has announced new additions to its portfolio of tools for COVID-19 research - two monoclonal antibodies that recognize the Spike RBD (B.1.1.529, Omicron Variant) protein and neutralizes its interaction with ACE2. The human ACE2 receptor is found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Research has shown that ACE2 mediates COVID-19 infection through direct binding of the SARS-CoV-2 Spike protein....

AMSBIO has announced a new range of kits, media and reagents to assist in the development of cultured meat products - an exciting new area of food technology. Cultured meat, also known as synthetic, artificial or in-vitro meat, is created by harvesting animal cells that are then placed in a bioreactor and fed with nutrients to help tissue growth. The driving force behind this rapidly expanding area of food technology is that the production of cultured meat requires far fewer animals to be farmed...

Global provider of human and animal biospecimens - AMSBIO offers a custom procurement service enabling researchers to source specific biofluid and tissue samples which are not already available in their extensive biorepository. To support increasingly complex research, AMSBIO aims to provide a full range of blood-based products, including whole blood, serum, plasma, blood and peripheral blood mononuclear cells (PBMCs)....

AMSBIO has launched a new range of research grade biosimilars that enable analysis of biological processes without the need to purchase expensive therapeutic-grade biologics. Investigating the biological effects of a drug or doing proof of concept assay development, requires a source of the drug. Typically, it is difficult to obtain original pharmaceutical grade biotherapeutic drugs for research use, rather than a clinical application....

AMSBIO has established an unmatched commercial biorepository and procurement network that includes a large selection of fully characterized and annotated Formalin-Fixed Paraffin-Embedded (FFPE) tissue blocks to help streamline your research. Drawing upon decades of experience - AMSBIO is able to offer an extensive collection of frozen and paraffin-embedded (FFPE) human and animal tissue samples from normal and many diseased tissue types....

AMSBIO invite the global research community to the 2021 annual meeting of the ISSCR, the leading professional organization of stem cell scientists, to discover how iPSCs and organoids can be used in COVID-19 drug discovery research. At this virtual meeting - Dr. Kazuo Takayama from the renowned Center for iPS Cell Research and application (CiRA) at Kyoto University will be giving a unique insight into his recent pioneering research...

AMSBIO reports upon a publication that cites how its STEM-CELLBANKER® animal-free cryopreservation media has played a role in the development of a cell therapy for Parkinson’s Disease that will soon be going into clinical trials. Parkinson’s disease is one of the most common neurodegenerative diseases worldwide. Its main features include motor symptoms such as bradykinesia, rigidity, resting tremor, and postural instability, though non-motor symptoms are often also present....

AMSBIO has launched two new biotinylated formats of the 10E4 Heparan Sulfate antibody (high and low biotin) along with a starter pack to allow scientists to test both formats at a reduced cost. Heparan Sulfate (HS) is a highly sulfated linear polysaccharide that is attached to a core protein to form HS Proteoglycans on the cell membrane or in the extracellular matrix....

AMSBIO has introduced a collection of mutated SARS-CoV-2 spike proteins and recombinant antigens for the new virus variants - B.1.1.7 and B.1.351. These reagents can be used by researchers to evaluate the efficacy of the antibodies and identify targets for development of improved therapeutic drugs and vaccines. Emerging in the latter part of 2020, multiple new SARS-CoV-2 variants are now circulating globally....

AMSBIO has introduced a series of kit products based upon high-quality SARS-CoV-2 antigen proteins to improve the efficiency of anti-SARS-CoV-2 drugs and vaccines development. These new kit products include SARS-CoV-2 inhibitor screening kits, antibody titer serologic assay kits, antigen detection kit and antigen pre-coated plates....

AMSBIO has published an interview with researchers at Yamaguchi University Graduate School of Medicine, Department of Gastroenterology and Hepatology, Japan, that covers their groundbreaking work to develop cell regeneration therapies for liver cirrhosis. The interview covers the involvement of the Graduate School of Medicine in the development of StemFit® for Mesenchymal Stem Cell (MSC), a new chemically defined medium which minimizes the risk of hazardous animal derived ingredients....

Using HS antibodies from AMSBIO, researchers have demonstrated that Heparanase enzyme contributes to the pathogenesis of COVID-19, with both Heparanase activity and HS levels being elevated in plasma of Covid-19 patients. In addition, recent published data has shown that Heparanase activity is further elevated in ICU patients on ventilators compared to less serious cases....

AMSBIO has further expanded its product range to help in the fight against COVID-19 with the introduction of a new range of SARS-CoV-2 antibodies and antigens to aid development of new immunoassay diagnostic kits. While RT-PCR diagnostic kits has provided fast and sensitive COVID-19 case confirmation, the technique suffers from some inherent drawbacks that limit it to diagnosis during the acute phase of infection....

AMSBIO announce new additions and certifications for their market-leading range of clinical grade, chemically defined cryopreservation excipient solutions - STEM-CELLBANKER® and HSC-BANKER®. STEM-CELLBANKER® DMSO Free GMP grade is a new chemically defined freezing solution that does not contain DMSO as an anti-freezing agent. It was developed for customers who prefer not to use DMSO-containing cryopreservation solution...

AMSBIO announce a new service designed to provide high-content cytometry data on cells and tissues enabling the automated analysis of fluorescent biomarkers on living cells or long-term stored biobanked cells and tissues. Leveraging the innovative ChipCytometry platform, AMSBIO is able to offer a complete workflow solution (products and services) for high-content cytometry on cells and tissues, from sample preparation and biobanking to biomarker analysis and data mining....

Exosomes are small endosome derived lipid nanoparticles, actively secreted by exocytosis in most living cells. Acting on tissues in both the local environment and further afield, they function as important signal carriers through their transport of RNA, proteins, lipids and DNA. Exosomes are involved in a wide range of normal and pathogenic processes, making exosome research crucial in multiple areas....

AMSBIO has launched a range of ready-to-use SARS-CoV-2 Spike protein coupled magnetic beads that combine convenience, minimum non-specific binding and developed operational protocols saving you time and hassle. Developed for immunocapture, cell stimulating, biopanning and flow cytometry applications these new products use biotinylated SARS-CoV-2 Spike protein pre-coupled to streptavidin conjugated magnetic beads, which can capture the Anti-SARS-CoV-2 antibody or ACE2 protein from cell or serum sample....

AMSBIO reports on the pioneering work by researchers at the University of Sheffield, UK to measure the interaction between the SARS-CoV-2 Spike S1 protein receptor binding domain (SARS-CoV-2 S1 RBD) and heparin. This has resulted in a new assay which could be a useful first screen for novel inhibitors of coronavirus infection. Evidence is mounting that SARS-CoV-2 uses heparan sulfates (HS) during the initial attachment to hosts cells, and this interaction appears to increase the affinity for the critical receptor, ACE2....

The MagSi-NA Pathogens Kit from AMSBIO enables easy extraction of DNA and RNA from serum, plasma, oropharyngeal swab / nasopharyngeal swab, and any other respiratory samples for pathogen detection. The kit is particularly suitable for total nucleic acid extraction for SARS-CoV-2 / COVID-19 detection, currently a major bottleneck in COVID-19 testing....

AMSBIO announce the availability of recombinant proteins and antibodies for detection of Coronavirus (COVID-19) and related RNA viruses. Historically strains of Coronavirus able to infect humans (HCoV-OC43, HCoV-229E, HCoV-NL63, and HCoVHKU1) were not highly pathogenic and only caused mild respiratory illness. By comparison more recent strains - SARS-CoV, MERS-CoV and COVID-19 that emerged in Wuhan, China at the end of 2019 are highly pathogenic resulting in large scale pandemics....

The EZ PREP Peripheral Blood Mononuclear Cell Isolation Kit from AMSBIO is designed to efficiently isolate highly purified peripheral blood mononuclear cells (PBMCs) from fresh whole blood products by density gradient centrifugation. The unique EZ PREP tube design provides greater visibility of your target cell population, allowing you to maximise your recovery of mononuclear cells. As a result, PBMCs are immediately available for downstream applications such as flow cytometry, culture, and/or DNA/RNA isolation...

ScreenIn3D, a partner of AMSBIO, has been awarded the prestigious ELRIG Prize for Innovation in Drug Discovery at their flagship conference Drug Discovery 2019 which recently took place in Liverpool, UK. ELRIG Drug Discovery has become the largest meeting of life sciences industry professionals in the UK. The 2-day event brings together more than 1200 delegates and over 100 exhibiting companies from around the world to highlight the latest ground-breaking research....

AMSBIO reports on a paper from the Malmberg Lab at Oslo University Hospital and the Norwegian Radium Hospital Institute for Cancer Research (Norway) that suggests a mechanism by which Natural Killer (NK) cell education operates through modulation of the lysosomal compartment under the influence of inhibitory receptor–ligand interactions....

AMSBIO has introduced a new range of homogeneous proximity assays, critical enzymes and small molecule inhibitors to assess the chemical adaptor function of Proteolysis Targeting Chimeras (PROTACs). Targeted protein degradation using PROTACs is a new and promising therapeutic method in drug discovery....

AMSBIO announce new, best-in-class CAR-T cells, engineered CAR-T target cells, PBMC, cell media and activation beads in response to the rapid growth in research on the therapeutic uses of CAR-T cells. All CAR-T cell products from AMSBIO are T cells produced from either a group of donors or from individual donor transduced with CAR (Chimeric antigen receptor) - lentivirus....

ScreenIn3D, a partner of AMSBIO, has received a share of the £1 million plus prize pot and will receive ongoing support from partners of Scottish Edge - a respected regional business support agency. Scottish EDGE runs a competition aimed at identifying and supporting Scotland’s up-and-coming, innovative, high-growth potential entrepreneurial talent....

AMSBIO reports on the pioneering research published in a collaborative effort between researchers in the School of Engineering, University of Newcastle and Department of Pharmacology, University of Cambridge, UK that has cited Biotinylated Hyaluronan Binding Protein (biotin-HABP) supplied by the company in its investigation into the cancer resistance of the naked mole-rat (NMR)....

AMSBIO introduces StemFit® Basic04, a next generation feeder-free medium for the maintenance of Induced Pluripotent Stem (iPS) and Embryonic Stem (ES) cells during the reprogramming, expansion and differentiation phases of stem cell culture. With its fully defined and animal-origin free formulation, StemFit® Basic04 is suited to both research studies and translation to cell therapy. With the new StemFit® Basic04 it is now possible to experience...

AMSBIO announces XerumFree™- a new concept in cell culture that allows you to culture cells without the use of serum. XerumFree™ has been developed taking into account what is missing in traditional basal cell culture media to sustain growth of all cell types, which is a nutritional approach at the cellular level. Containing no animal or human derived material, XerumFree™ is a serum....

AMSBIO reports on how several leading European research groups have used their Lipidure® coated microplates to help culture physiologically functional, light-responsive retinal organoids. Development of in vitro models of the human retina in which to perform pharmacological and toxicological studies is an urgent area of vital research. An essential step for developing in vitro models of human retina is the ability to generate laminated....

AMSBIO offers an unmatched portfolio of over 2000 tissue microarrays (TMAs), one of the best sample formats for rapid detection of cellular localization of RNA and protein expression. Recently AMSBIO introduced US Food & Drug Administration (FDA) standard TMAs for human, mouse and rat to its portfolio. Designed in close conformance with FDA guidelines, this range meets the requirements for tissue cross-reactivity studies required for therapeutic and diagnostic antibody and antibody-like...

AMSBIO has added a new range of GCaMP calcium indicators (jGCaMP7b, jGCaMP7c, jGCaMP7f, jGCaMP7s) to their portfolio of ready-to-use adeno-associated virus (AAV) biosensor products. These viruses encoded biosensors are supplied ready for use for neural activity studies in vivo. AAV Biosensors provide a snapshot of intracellular interactions, specifically active brain circuitry. Using fluorescent protein-based AAV...

Detachin™ Cell Detachment Solution from AMSBIO is a high-performance alternative to Trypsin/EDTA for detaching adherent cells from in vitro growth vessels. Detachin™ provides rapid, gentle, and effective detachment of a wide variety of adherent cells, including primary cells, from all commercially available tissue culture plasticware. Proven to provide a consistent, safe, and efficient method for cell detachment and dissociation....

AMSBIO has established a comprehensive portfolio of knock-out cell lines and lysates generated with CRISPR/Cas9 technology. Using CRISPR/Cas9 gene editing technology for the generation of knock-out cell lines and cell lysates ensures the development of useful tools to validate antibody specificity and elucidate gene function. Knock-out cell lines and cell lysates are widely agreed to be the best negative controls to quickly and confidently...

AMSBIO is a leading supplier of gold, silver and platinum coated substrates for nanotechnology researchers looking to use these surfaces to characterize self-assembled monolayers using atomic force microscopy (AFM), or scanning tunneling microscopy (STM). Gold, silver and platinum surfaces offer a clean, near atomically-flat surface that is ideal for applications in nanotechnology, such as studies on self-assembly, single-molecule imaging, nano- or micro-contact printing, DNA origami, or nanophotonics....

AMSBIO has added new experimental cell lines to its CAR T cell research range. Recent FDA approval of CAR T cells for treatment of leukemia and lymphoma has resulted in rapid growth in research on the therapeutic uses of such cells. The new AMSBIO experimental cell lines provide you with the power to screen antibodies and ligands against your target, enhancing your search for new CAR T therapies....

AMSBIO, a leading global supplier of Chondroitinase ABC enzyme, reports on the recent publication by Hu et al. of the therapeutic efficacy of microtube-embedded Chondroitinase ABC on a group of dogs with naturally occurring spinal injuries. Developing new therapies for human spinal cord injuries is a challenging task because the damage is heterogeneous in terms of spinal location and severity. Ideally a new therapy should work for survivors with...

AMSBIO has expanded its range of Wingless/Integrated (Wnt) recombinant proteins, stable reporter cell lines, antibodies and ELISA kits to assist researchers in the fields of developmental biology and oncology. The Wnt family of cysteine-rich secreted polypeptides comprise more than 16 mammalian family members involved in several important cell functions such as cell cell-cell communication, proliferation...

AMSBIO introduces ALiCE® - a new high yield cell-free protein expression system. Cell-free protein expression (CFPE) is today used by protein chemists to quickly produce small amounts of proteins when screening DNA libraries. However current technologies are limited and there is a need for higher protein expression yields. ALiCE® is a unique eukaryotic system, which yields an unprecedented 3 mg/ml of protein in batch mode...

AMSBIO announces that induced pluripotent stem (iPS) cell expert - Dr Masato Nakagawa from the Nobel Prize winning Yamanaka Lab at the Centre for iPS Cell Research and Application, Kyoto University, Japan will give 3 talks in the UK on the 10th and 11th December 2018. Appearing at the Wellcome Genome Campus (Cambridge), the MRC Centre for Regenerative Medicine (Edinburgh) and at Guys Hospital (London)...

AMSBIO has added an important new product to its immunotherapy range - a PD-L2 / TCR activator - CHO Recombinant Cell Line. PD-L2 is one of the key players in immune checkpoint therapy, which is the focus of the 2018 Nobel Prize for Physiology & Medicine . This work pioneered by Professor James P Allison from the USA and Professor Tasuku Honjo from Japan, has led to treatments for cancers, such as melanoma, lung cancer and others based upon immune checkpoint therapy...

ScreenIn3D, a partner of AMSBIO, reports on the accolades it has received following the recent launch of its novel microfluidic cancer screening service. Combining the latest advances in microfluidics and 3D culture - the service uses an optimised screening platform targeted at improving anticancer drug treatment and accelerating development of new personalised medicine solutions. At the recent Miptec conference in Basle, Switzerland - ScreenIn3D was one of 60 global organisations invited to showcase its cutting-edge life science technologies....

AMSBIO announce the launch of a new range of circulating tumor DNA (ctDNA) Reference Standards for cancer disease management research. A critical goal in cancer disease management is early detection. When detected early, cancer progression can be arrested using techniques such as targeted therapy, chemotherapy and surgery thereby increasing the chances of survival and a higher quality of life. Liquid biopsy offers the promise of providing a quick Liquid biopsy offers the promise of providing a quick, non-invasive and cost-effective alternative...

AMSBIO produce a growing range of experimental cell lines, recombinant proteins and screening services to advance the search for new Chimeric Antigen Receptor (CAR T) cell therapies to treat cancers. For years, the foundations of cancer treatment were surgery, chemotherapy, and radiation therapy....

The TUNR Flexible Gene Editing System from AMSBIO allows you to precisely control the expression of your gene of interest. Leveraging a CRISPR-Cas9 system for targeted integration of a polyA track at a precise location within the targeted gene, our kits are custom-designed for your project and include everything you need to tune gene expression in your favorite cell line. AMSBIO TUNR kits allow you to...

AMSBIO has announced an exosome isolation, manufacturing and characterization service to enable R&D groups to accelerate their discovery and development activities. Exosomes are small membrane vesicles with a size ranging from 40 to 100 nm. They are known to serve as functional mediators in cell interaction leading to cancer metastasis and have also been implicated in neurodegenerative diseases....

AMSBIO has introduced new CRISPR / Cas9 editing kits for gene knock-out, gene knock-in and gene mutation applications. The latest tool in genome science – CRISPR/Cas9 is a simple and efficient genome editing tool which allows for specific genome disruption and replacement resulting in high specificity and low cell toxicity.

AMSBIO announces MyEZGel™ 3D-iPSC Matrix - a powerful new tool for in vitro 3D human induced pluripotent stem cell (hiPSC) culture, enabling more accurate in vivo predictions in life science research and development. MyEZGel™ 3D-iPSC is a synthetic xeno-free hydrogel matrix inspired by muscle and spider silk proteins....

ScreenIn3D (Glasgow, UK) is a new joint venture company set up by AMSBIO and the University of Strathclyde exploiting the latest advances in microfluidics and 3D culture to develop a novel microfluidic screening platform. he unique combination of microfluidic lab-on-a-chip technology with physiologically relevant 3D spheroids is designed to make drug discovery more closely relevant with what happens in the body...

AMSBIO announces MyEZGel™ 3D-iPSC Matrix - a powerful new tool for in vitro 3D human induced pluripotent stem cell (hiPSC) culture, enabling more accurate in vivo predictions in life science research and development. MyEZGel™ 3D-iPSC is a synthetic xeno-free hydrogel matrix inspired by muscle and spider silk proteins. Beneficially, MyEZGel™ is proven to overcome basic problems associated with traditional...

AMSBIO announces the launch of StemFit® Basic03 clinical grade stem cell culture for regenerative medicine. Provided as a animal-origin free, ready to use media for cell therapy applications, StemFit® AK03N which is the Japanese version of Basic03 has already been accepted by Pharmaceuticals and Medical Devices Agency, Japan (PMDA) for retinal regenerative medicine clinical trials....

AMSBIO, a global provider of gene editing and personalized medicine technologies, has launched a service for high throughput gene expression analysis. Based on the NanoString nCounter platform, the service works with a large array of sample types, including formats such as formalin-fixed paraffin-embedded (FFPE), which have been difficult to analyse by more traditional quantitative PCR...

AMSBIO, a global provider of gene editing and personalized medicine technologies, has launched a service for high throughput gene expression analysis. Based on the NanoString nCounter platform, the service works with a large array of sample types, including formats such as formalin-fixed paraffin-embedded (FFPE), which have been difficult to analyse by more traditional quantitative PCR...

AMSBIO announces MC-Easy™ minicircle technology for labs looking to produce sustained transgene expression in model animal generation and gene therapy research without the risks of introducing exogenous DNA. Minicircles are circular DNA elements that no longer contain antibiotic resistance markers or the bacterial origin of replication....

AMSBIO announces MC-Easy™ minicircle technology for labs looking to produce sustained transgene expression in model animal generation and gene therapy research without the risks of introducing exogenous DNA. Minicircles are circular DNA elements that no longer contain antibiotic resistance markers or the bacterial origin of replication....

AMSBIO has signed an agreement with Genea Biocells (San Diego, USA) to become global distribution partner to the academic community for its groundbreaking skeletal muscle differentiation product range. Genea Biocells developed the world’s first protocol to robustly differentiate human pluripotent stem cells to skeletal muscle cells with high yields and without cell sorting or genetic manipulation (Caron et al. 2016)....

AMSBIO reports on ground breaking research by the Institute for Research in Biomedicine (IRB), Barcelona that cites use of its proprietary 3D organoid technology to develop a mouse model that mimics advanced human colon cancer. This model has allowed them to study the immune system response for the first time. In a short space of time, therapies based on...

AMSBIO, a transatlantic life sciences company, and the University of Strathclyde (Glasgow, UK) have pioneered a multidisciplinary approach combining expertise in microfluidics and 3D culture to develop a novel microfluidic platform for cancer research. The new platform combines microfluidic lab-on-a-chip technology with physiologically relevant 3D spheroids to enable formation and long-term culture of 3D multicellular tumors / organoids for drug screening and...

AMSBIO announces the launch of a new range of induced pluripotent stem cell (iPSC) derived neural stem cells (NSCs) and neural lineage progenitors.For several years, neuroscience research using cell line and animal models of degenerative neurological disorders such as Parkinson’s and Alzheimer’s disease has been challenged to translate results into in vivo conditions, whether for basic understanding of disease pathology or for the screening of targeted drug therapies....

AMSBIO has introduced recombinant Wnt3a with a new Wnt stabilizer that significantly extends the activity of this protein in serum-free medium from a half-life of just 2 hours to around 30 hours.

Wnt-3a is a protein that is encoded by the Wnt3a gene. The Wnt gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis and in several other developmental processes...

AMSBIO announces iMatrix recombinant Laminin-511 E8 fragments, an innovative cell culture matrix compatible with a wide variety of cell types, and exceptionally well suited for pluripotent stem cells.

Laminin-511 is a major component of the basement membrane, which is expressed in early development of the embryo and can be used as a matrix for pluripotent (ES/iPS) stem cells, as it binds to integrin on cell surfaces....

iMatrix recombinant Laminin-511 E8 fragments is an innovative cell culture matrix compatible with a wide variety of cell types, and exceptionally well suited for pluripotent stem cells.

Laminin-511 is a major component of the basement membrane, which is expressed in early development of the embryo and can be used as a matrix for pluripotent (ES/iPS) stem cells, as it binds to integrin on cell surfaces....

HSC-BANKER® from AMSBIO is a new optimised GMP grade cryopreservation medium for the banking of hematopoietic stem cells isolated from umbilical cord blood, peripheral blood and bone marrow.    

In a comparative study on the cryopreservation of hematopoietic stem cells, performed by the Cord Blood Bank of the Japanese Red Cross Society, results using HSC-BANKER® were shown to at least equivalent to conventional protocols using DMSO and DEXTRAN. Manufactured to be completely free of serum and animal derived components, HSC-BANKER® contains only European or US Pharmacopoeia graded ingredients making it suitable for storage of...

AMSBIO has introduced a new range of Herpes Virus Entry Mediator (HVEM) products for use in immuno-oncology research. 

These new products will be of great interest to researchers studying TNFR family members such as CD137 (4-1BB), GITR, OX40, CD27, and other co-stimulatory proteins involved in B and T cell development, immune activation, and antitumor immune responses. The AMSBIO range includes a suite of HVEM products....

AMSBIO announces the launch of a new service for synthesis of custom-designed, highly modified mRNAs prepared by an advantageous in vitro transcription methodology.

Traditional approaches for engineering changes in cellular expression profiles have employed mostly DNA or RNA based viral and non-viral vectors. Unfortunately, these methods carry high risks, due to genomic integration with permanent genetic alteration of cells....

AMSBIO announce the addition of mutation cell line Formalin-Fixed Paraffin-Embedded (FFPE) samples and isolated genomic DNA (gDNA) controls to its extensive range of cell line reference standards.

In both routine clinical use and diagnostic assay development, biologically-relevant positive and negative controls represent an invaluable resource. To ensure accuracy and reproducibility during IVD assay development and established diagnostic tests, reference standards for genomic DNA analysis and Immunohistochemistry....

AMSBIO announce the addition of mutation cell line Formalin-Fixed Paraffin-Embedded (FFPE) samples and isolated genomic DNA (gDNA) controls to its extensive range of cell line reference standards.

In both routine clinical use and diagnostic assay development, biologically-relevant positive and negative controls represent an invaluable resource. To ensure accuracy and reproducibility during IVD assay development and established diagnostic tests, reference standards for genomic DNA analysis and Immunohistochemistry (IHC) assays are an essential tool....

AMSBIO has announced a new range of CD47-relevant products suitable for rapid high throughput screening studies.

CD47, or Integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. Studies have established that CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion and migration.....

AMSBIO announces the availability of StemFit® Basic02feeder-free stem cell culture media.

Stem cells and genome editing offer exciting opportunities within regenerative medicine. However, any clinical application of stem cells requires strict regulation to ensure that the cells are not exposed to  animal derived products. StemFit® Basic02 is a xeno-free, defined medium for human pluripotent stem cell (hiPSC) culture....

AMSBIO announces its sponsorship of the 'Discovering Organoids: Establishment, Culture & Manipulation’ hands-on training course due to be hosted at Homerton College, University of Cambridge, UK, on 11th and 12th September 2017.

Attendees to the 2-day intensive course will receive world-class theoretical instruction and hands-on practical training from leaders in the field of Organoid technology. The course will also cover specialist approaches for the application of CRISPR gene editing technology to Organoid cultures....

AMSBIO reports on an independent evaluation and use by I-Stem (Corbeil-Essonnes, France) of the Skeletal Muscle Differentiation kit - a cell culture media system to differentiate human stem cells into functional myotubes.

I-Stem was formed in 2005 through a collaboration between Inserm, the French National Institute of Health and Medical Research, and AFM-Telethon the French Association against Myopathies. I-Stem is the largest French laboratory for research and development dedicated to human pluripotent stem cells....

AMSBIO announces the launch of a new Hyaluronic Acid Quantification Kit, based on a highly specific and tightly binding protein to detect and measure Hyaluronic Acid, also known as Hyaluronan or HA.

This product is a competitive ELISA-like kit using a recombinant Hyaluronic Acid Binding Protein (rHABP) optimized to quantify HA polymers of average molecular weight greater than 7.4 kDa in samples such as serum, plasma and culture supernatant....

AMSBIO announces the launch of DermaChip® - a new high throughput assay technology that allows scientists, for the first time, to reliably measure the genotoxic risk of a whole range of environmental agents (including cleaning agents, household products and industrial chemicals as well as cosmetics) to our skin.

Using risk assessment data from a micronucleus assay together with the traditional Comet analysis of cells isolated from treated 3D skin culture models has been shown to be a better predictor of skin toxicity compared to the standard battery of in vitro toxicity assays....

AMSBIO has introduced a range of purified cancer exosome samples to help researchers study the role of exosomes in cancer development and metastasis.

By targeted filtration of patient serum using a specific binding method removes protein contaminant to isolate exosome at 95% high purity. Using this proprietary filtration method, the purified tumor exosome does not contain any polymer or detergent (SDS, NP-40 or Triton). The purified tumor exosome can be used for RNA or protein analysis techniques such as qRT-PCR, microarray and mass spectrometry....

AMSBIO announces its new generation laboratory benchtop sterilizer - the CoolCLAVE™ Plus.

The CoolCLAVE™ Plus provides 20-25x more sterilization power than its popular predecessor (CoolCLAVE™) by using the power of combined UV and ozone cycles plus a new heating function. The CoolCLAVE™ Plus is highly effective in keeping sterile, or sterilizing contaminated pipettes, pipette tips, gloves, plates, small instruments as well as personal items such as keys, glasses and wallets....

AMSBIO offer a wide range of products for IDO pathway research, including cell lines, assay kits and active proteins to be used in IDO pathway research (IDO1, IDO2 and TDO genes).

Immune evasion is one of the identifying hallmarks of cancer, and researchers worldwide are trying to identify the complex mechanisms that enable cancer cells to evade the host’s immune system. Cancer cells use the indoleamine 2,3-dioxygenase (IDO) pathway to suppress the host’s immune response in order to facilitate survival, growth, invasion, and metastasis of malignant cells....

AMSBIO has introduced a new range of ready-to-use Reporter Lentivirus for assessing cell signaling activities in virtually any mammalian cell type. 

These pre-made products use a unique combination of transcription factor reporter technology coupled with lentiviral delivery to provide a simple, highly sensitive method capable of operating over a large dynamic range....

AMSBIO reports on an independent study that evaluated the use of CELLBANKER® and STEM CELLBANKER® reagents for cryopreservation and thawing of organoids.

Data is presented that clearly shows that liver organoids recovered and grew well in STEM-CELLBANKER®–GMP, CELLBANKER® 1 and CELLBANKER® 2 freezing media after being thawed out after 2 days and 4-days cryopreservation....

AMSBIO reports how, using its Mimetix® aligned 3D cell culture scaffold, researchers at the MRC Centre for Regenerative Medicine, University of Edinburgh (UK) have created a high throughput myelination assay that shows great promise as a model for research into Multiple Sclerosis.

Multiple sclerosis (MS) is a demyelinating disease in which the insulating covers of nerve cells in the brain and spinal cord are damaged. MS is a debilitating neurological condition that affects 2.5 million patients worldwide, 100,000 in the UK with 200 new cases diagnosed each week in the US....

AMSBIO has announced the launch of its new cfPure™ Cell Free DNA Purification Kit for scalable and automation-friendly isolation of circulating cell-free DNA (cfDNA) from patient serum or plasma samples.

Circulating cfDNA is normally comprised of DNA fragments from healthy cells.  However, apoptotic cells, necrotic cells, cancer cells, and intact cells all release cfDNA into the bloodstream. In addition, fetal cfDNA can be isolated from peripheral maternal blood. The utility and value of using cfDNA from serum and plasma for non-invasive molecular diagnostics.....

AMSBIO has announced the launch of its new cfPure™ Cell Free DNA Purification Kit for scalable and automation-friendly isolation of circulating cell-free DNA (cfDNA) from patient serum or plasma samples.

Circulating cfDNA is normally comprised of DNA fragments from healthy cells.  However, apoptotic cells, necrotic cells, cancer cells, and intact cells all release cfDNA into the bloodstream. In addition, fetal cfDNA can be isolated from peripheral maternal blood. The utility and value of using cfDNA from serum and plasma for non-invasive molecular diagnostics...

AMSBIO has published a new 36-page catalogue that details its comprehensive range of high purity reagents for proteoglycan and glycosaminoglycan research. 

Proteoglycans are glycosylated proteins which contain covalently attached highly anionic glycosaminoglycans (GAGs). Proteoglycans are present in different forms within different types of extracellular matrices and connective tissues. Heparan sulfate proteoglycans (HSPGs) are known to play important structural and functional roles in linking the component proteins of the basement membrane...

AMSBIO has published a new 36-page catalogue that details its comprehensive range of high purity reagents for proteoglycan and glycosaminoglycan research. 

Proteoglycans are glycosylated proteins which contain covalently attached highly anionic glycosaminoglycans (GAGs). Proteoglycans are present in different forms within different types of extracellular matrices and connective tissues. Heparan sulfate proteoglycans (HSPGs) are known to play important structural and functional roles in linking the component proteins of the basement membrane, controlling the permeability properties of the membranes and signal...

AMSBIO has introduced a new range of cancer cell line models and culture media for the most difficult to culture tumor types where no models may exist.

Alex Sim, Managing Director at AMSBIO said, "Our new partnership with Cellaria Biosciences expands our existing portfolio by introducing patient-derived cancer models; allowing customers to conduct research and drug discovery initiatives to advance precision medicine.” Directly derived from patient tumors without any genetic manipulation, these new products provide the assurance of primary cells....

AMSBIO has published a new 25-page Neuroscience catalogue that details its extensive range of specific tools and reagents to enable researchers stay at the forefront of their field.

Cellular models are key tools that open the door to numerous neuroscience applications including neurodegeneration, neurogensis and developmental diseases.  With the discovery that neural stem cells exist in the adult brain many researchers are now seeking to use these cells in in vitro studies....

AMSBIO announce a new range of ready-to-use adeno-associated virus (AAV) biosensor products.

These viruses encode your chosen biosensor, either calcium or glutamate sensor and are ready for in vivo injection. The new range of Calcium or Glutamate biosensor products comes with a choice of promoter and the ability to include the Cre inducible (FLEx-ON) expression. AMSBIO have packaged these indicators into the most commonly used AAV serotypes (AAV8 and AAV9)...

AMSBIO offers an extensive range of proteins, antibodies, assays and kits covering every single target of major cellular metabolic pathways including folate metabolism, pyruvate metabolism (with and without oxygen), citrate metabolism, O2 consumption and toxicity, oxidative stress and fatty acid oxygen measurement.

Cellular metabolism involves a series of interconnected biochemical reactions catalyzed by enzymes inside a cell, better known as metabolic pathways. These reactions convert a substrate molecule or molecules through a series of metabolic intermediates, eventually yielding a final product....

AMSBIO has introduced the MitoXpress® Xtra Fatty Acid Oxidation (FAO) kit to facilitate the convenient measurement of FAO-driven respiration. 

Fatty acid oxidation is the primary metabolic pathway in a variety of tissues, which is particularly important during periods of glucose deprivation. In organs such as liver and skeletal muscle, FAO can provide over 75% of cellular ATP while in cardiac tissue it can be responsible for up to 90% of cellular energy requirements....

AMSBIO reports on the recent publication in Nature Protocols by Dr Meritxell Huch and co-workers which describes development of culture conditions that allow the long-term expansion of adult primary tissues from the liver and pancreas into self-assembling 3D organoid cultures.

Traditionally adult somatic tissues have proven difficult to expand in vitro, largely because of the complexity of recreating appropriate environmental signals in culture. Huch** and co-workers have overcome this problem and describe recently established protocols for the long-term (> 1 year) expansion and genetic manipulation of adult liver and pancreas cells in 3D organoid culture...

AMSBIO announces a suite of high quality proteins and biotinylated proteins for research into killer-cell immunoglobulin-like receptors.

Killer-cell immunoglobulin-like receptors (KIRs), are a family of type I transmembrane glycoproteins expressed on the plasma membrane of Natural Killer (NK) cells and a minority of T cells. They regulate the killing function of these cells by interacting with major histocompatibility (MHC) class I molecules, which are expressed on all nucleated cell types. KIR receptors can distinguish between MHC class I allelic variants, which allows them to detect virally infected cells or...

AMSBIO has expanded its wide and varied catalogue of primary and progenitor cell types and media with a new range of human endothelial progenitor cells (EPC).

Human endothelial progenitor cells are a primitive cell type. They are bone marrow derived cells with properties similar to embryonic angioblasts. Progenitor cells migrate into the blood stream and are able to differentiate into a variety of mature vascular endothelial cell types....

AMSBIO announces an extended and updated edition of its popular Organoid Culture Handbook.

Launched at the EMBO Symposium on Organoids: Modelling Organ Development and Disease in 3D Culture, the new 24-page handbook includes valuable information about the latest available products, protocols, example results and research paper citations. Within the protocols section detailed information is provided on a general submerged method for organoid culture, crypt organoid culture techniques...

AMSBIO has launched a comprehensive selection of products for Wnt signaling research including recombinant proteins, stable reporter cell lines, polyclonal antibodies and ELISA assay kits.

Wnt proteins are a family of cysteine-rich secreted polypeptides involved in important cell functions including cell to cell communication, cell proliferation, cell migration, cell polarity, survival and self-renewal. In particular Wnt3a signals play an important role in the ability of organoids to expand. Additionally, loss of activation of Wnt expression has been associated with alteration of cell fate, morphogenesis and mitogenesis which has considerable relevance in...

AMSBIO has produced a new 24-page guide that brings together applications, protocols, results and citations to provide an informative resource for researchers using heparan sulfate antibodies.

Heparan Sulfate (HS) is synthesized as the glycosaminoglycan (GAG) component of heparan sulfate proteoglycans (HSPGs). It is expressed on the cell surface of virtually all cell types and basement membranes. It displays specific interactions with many biologically active proteins and, thus is involved in many important biological processes....

AMSBIO announces MagSi-DNA Vegetal - a powerful kit that enables efficient extraction of genomic DNA from plant tissue.

The new MagSi-DNA Vegetal kit brings the convenience and cost effectiveness of magnetic bead technology to the world of plant genomics.  Whether you work with DNA extracted from flowers, grain crops, fruits, vegetables, trees, or other plant materials, MagSi-DNA Vegetal is designed to maximize your effectiveness.....

AMSBIO has introduced Cultrex® Organoid Progenitor Cells that have been derived from normal, healthy mouse small intestine tissue and are continuously cultured using Reduced Growth Factor BME R1 and BME 2.

This new introduction extends the range of products offered by AMSBIO to support organoid culture. It will be of particular interest to the large number of leading research groups worldwide using BME 2 organoid matrix that specialise in drug screening applications....

Anglo-American life science firm AMSBIO announces a new range of recombinant antigens and antibodies for Zika virus, which are suited for the development of rapid assays and to overcome the problem of cross-reactivity with related viruses.

The Zika virus (ZIKV), which is borne by mosquitos, is causing worldwide concern because of its connection to a neurological birth disorder and its rapid spread across the globe. The imminent 2016 Olympic Games in Brazil, where the epidemic started, is as a consequence suffering withdrawals from a growing number of competitors....

AMSBIO has introduced new CRISPR gRNA Lentivector Cloning Kits that provide scientists with a targeted and precise genomic gene editing methodology.

Targeted and precise genomic gene editing technologies are today key tools for genomic correction, modification and gene therapy.  The recently discovered CRISPR/Cas gene editing technique offers higher targeting accuracy, much more target sequence selection, much less complexity and less off-target cell toxicity than the previous generation genome editing technologies. To enable CRISPR genomic editing, the AMSBIO kit...

AMSBIO has introduced new cell lines and controls for Programmed Cell Death Protein 1 (PD-1) research, and to test inhibitors of the PD-1 / PD-L1 pathway.

The binding of PD-1, a receptor expressed on activated T-cells, to its ligands (PD-L1 and PD-L2) negatively regulates immune responses. PD-1 ligands are highly expressed in many cancers, and their interaction inhibits T-cell activity allowing cancer cells to escape immune surveillance. The PD-1: PD-Ligands pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for...

AMSBIO has launched a range of new TIGIT receptor products including a Jurkat cell line, Homogeneous assay kits and recombinant proteins for immunotherapy research.

Human T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is a receptor expressed on the surface of human T cells and NK cells that binds to CD155 and CD112 present on the surface of dendritic cells. The binding of TIGIT with CD155 or CD112 results in inhibition of T cell and NK cell activation....

AMSBIO has introduced new cell lines and controls for Programmed Cell Death Protein 1 (PD-1) research, and to test inhibitors of the PD-1 / PD-L1 pathway.

The binding of PD-1, a receptor expressed on activated T-cells, to its ligands (PD-L1 and PD-L2) negatively regulates immune responses. PD-1 ligands are highly expressed in many cancers, and their interaction inhibits T-cell activity allowing cancer cells to escape immune surveillance....

AMSBIO has introduced a wide selection of new high quality products for exosome research.

Formulated to be quick, affordable and easy to use, AMSBIO's new expanded suite of quantification kits, isolation tools, standards, DNA & RNA extraction kits, antibodies and cell culture reagents will facilitate your understanding of exosomes using a wide variety of samples. Exosomes are small endosome derived lipid nanoparticles actively secreted by exocytosis in most living cells. Release of exosomes occurs either constitutively or...

AMSBIO has launched a range of new TIGIT receptor products including a Jurkat cell line, Homogeneous assay kits and recombinant proteins for immunotherapy research.

Human T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is a receptor expressed on the surface of human T cells and NK cells that binds to CD155 and CD112 present on the surface of dendritic cells. The binding of TIGIT with CD155 or CD112 results in inhibition of T cell and NK cell activation. Antibodies and other agents that inhibit this signaling pathway have known to increase the immune response, particularly...

AMSBIO has introduced a wide selection of new high quality products for exosome research.

Formulated to be quick, affordable and easy to use, AMSBIO's new expanded suite of quantification kits, isolation tools, standards, DNA & RNA extraction kits, antibodies and cell culture reagents will facilitate your understanding of exosomes using a wide variety of samples. Exosomes are small endosome derived lipid nanoparticles actively secreted by exocytosis in most living cells. Release of exosomes occurs either constitutively or...

AMSBIO has introduced MitoXpress® Intra, a novel intracellular oxygen assay based on a 96 or 384-well fluorescence plate reader-based approach that facilitates the real-time assessment of transient changes in cell respiration, oxygen gradients and physiological responses across a range of cell models. 

Molecular oxygen is the key substrate of aerobic metabolism. Knowledge of cell oxygenation is therefore central to a detailed understanding of the cellular metabolic response to a particular treatment or manipulation. Traditionally intracellular measurements have proven very difficult, requiring the use of invasive, laborious, low-throughput, technically challenging techniques which in turn have limited the use of...

AMSBIO has introduced two new products to assist the increasing number of scientists adopting organoid culture as a physiologically relevant model for organ development.

These new tools build upon the widespread use of AMSBIO’s BME2 ‘organoid matrix’ by leading research groups worldwide. BME 2 is the extracellular matrix of choice for growing and supporting organoid cultures that are increasingly being adopted in drug screening applications. A new R-Spondin1 cell line has been developed that provides a cost-effective solution by which labs can obtain a long-term supply of R-Spondin1...

AMSBIO has introduced two new products to assist the increasing number of scientists adopting organoid culture as a physiologically relevant model for organ development.

These new tools build upon the widespread use of AMSBIO’s BME2 ‘organoid matrix’ by leading research groups worldwide. BME 2 is the extracellular matrix of choice for growing and supporting organoid cultures that are increasingly being adopted in drug screening applications. A new R-Spondin1 cell line has been developed that provides a cost-effective solution by which labs can obtain a long-term supply of R-Spondin1...

AMSBIO has launched the 96-Well CometChip® System - a high-throughput platform developed to simultaneously treat and measure DNA damage induced by different treatments, or among different cell types on a single slide using the Comet assay.

DNA damaging agents can promote aging, disease and cancer and are widespread in the environment as well as being produced within human cells as normal cellular metabolites. The ability to quantify DNA damage responses is consequently critical to researchers in public health, pharmaceutical or clinical labs. The CometChip® is based upon the well-established single cell gel electrophoresis “Comet Assay”, which...

AMSBIO has released a new 16-page practical guide that provides a detailed overview on how blockers can be used to reduce non-specific binding in immunoassays. 

Immunoassay Blocking Reagents (Blockers) are typically applied to ELISA and Lateral Flow-based assays, specifically paired monoclonal sandwich assays, competitive assays and antigen capture (IgG/IgM) assays. They work by removing interfering proteins in patient samples that could produce false results and an incorrect diagnosis. Choosing the best assay format depends on the intended application of the...

AMSBIO has released a new 16-page practical guide that provides a detailed overview on how blockers can be used to reduce non-specific binding in immunoassays.

Immunoassay Blocking Reagents (Blockers) are typically applied to ELISA and Lateral Flow-based assays, specifically paired monoclonal sandwich assays, competitive assays and antigen capture (IgG/IgM) assays. They work by removing interfering proteins in patient samples that could produce false results and an incorrect diagnosis. Choosing the best assay format depends on the intended application of the...

AMSBIO announces an extensive range of primary human cancer cells obtained directly from a variety tumor types, including breast, colon and prostate.

Despite significant advances in cancer research and treatments, the eradication of the disease remains elusive. This has led to a more urgent focus on precision medicine and advanced treatments like immunotherapy and the use of oncolytic viruses. Donor derived primary tumor cells offer a more in vivo like model potentially enhancing the development of targeted personalized therapeutics. It is known that using...

AMSBIO announces an extensive range of primary human cancer cells obtained directly from a variety tumor types, including breast, colon and prostate.

Despite significant advances in cancer research and treatments, the eradication of the disease remains elusive. This has led to a more urgent focus on precision medicine and advanced treatments like immunotherapy and the use of oncolytic viruses. Donor derived primary tumor cells offer a more in vivo like model potentially enhancing the development of targeted personalized therapeutics. It is known that using immortalized...

AMSBIO has announced two new additions to itsMagSi rangemagnetic bead-based products for protein purification, proteomics, and genomics applications.

Offering 100% compatibility with existing protocols, using magnetic bead products from the leading supplier, the clinical grade AMSBIO MagSi kits require little or no re-validation and offer substantial savings in cost. MagSi-NGSPREP Plus offers a simple and quick way to ensure consistent sequencing results. The new kit has been developed to provide an efficient solution for both size selection and clean-up of the successive enzymatic...

AMSBIO announces the availability of the world's first kit to differentiate human pluripotent stem cells into functional myotubes.

The new kit utilizes a highly efficient media based protocol to produce skeletal muscle cells from stem cells in a simple, scalable manner. The potential to differentiate stem cells into specific cell types is revolutionizing life sciences, from new methods of studying developmental biology and novel approaches to producing accurate disease models to techniques to help drug discovery and toxicity testing...

AMSBIO announces the launch of the Oris™ 3D Embedded Invasion Assay – a unique product offering quantifiable real-time analysis of cell invasion.

This new cell invasion assay is designed to enable cell movement through an extracellular matrix (ECM) in 3-Dimensions, thereby getting you as close as possible to an in vivo experiment using in vitro conditions. The invasion capacity of tumor cells is of particular concern in cancer research, and is a first step to metastasis, the deadliest aspect of the disease. Traditionally, cell invasion assays are performed by seeding cells in...

AMSBIO announces an extensive collection of recombinant Fc receptor proteins, including their common variants, to help accelerate the development of therapeutic monoclonal antibodies (mAb).

Therapeutic antibody efficacy depends not only on the Fab fragment and its binding activity to the target antigen, but also depends on the Fc fragment and its interaction with key Fc receptors. As a consequence screening for desired binding affinity to the key Fc receptors is an essential component in the development of a high quality therapeutic mAb. Recombinant Fc Receptor proteins from AMSBIO are uniquely suitable for...

AMSBIO announces the launch of the Oris™ 3D Embedded Invasion Assay – a unique product offering quantifiable real-time analysis of cell invasion.

This new cell invasion assay is designed to enable cell movement through an extracellular matrix (ECM) in 3-Dimensions, thereby getting you as close as possible to an in vivo experiment using in vitro conditions. The invasion capacity of tumor cells is of particular concern in cancer research, and is a first step to metastasis, the deadliest aspect of the disease. Traditionally, cell invasion assays are performed by seeding cells in a...

AMSBIO has assembled an extensive collection of autophagy antibodies.

From the industry standard autophagy antibody APG8 (MAPLC3) to the newest autophagy antibodies such as LAMP and APG1, AMSBIO is able to provide the most relevant, qualified antibodies for autophagy research. Autophagy is a cellular process not only implicated in the death of a cell, but more widely implicated in the survival of a cell. Autophagy functions as a housekeeping mechanism through disposal of aging and/or dysfunctional proteins and organelles by isolating...

AMSBIO has launched a comprehensive collection of pre-packaged human full-length cDNA ORF and miRNA adenoviruses.

Featuring over 1,240 pre-packaged human miRNA precursor adenoviruses and 30,000 pre-packaged human full-length cDNA ORF adenoviruses the collection will considerably enhance high throughput drug discovery, improve functional genomic studies, and make life science research more cost-effective and efficient. Recombinant adenoviruses are extremely efficient gene delivery systems that are widely used in human gene therapy...

AMSBIO offers a comprehensive range of more than 54,000 lentivirus for shRNA delivery.

To make selection of the optimum lentivirus quick and easy, AMSBIO has developed an online search tool** where you need simply enter your target gene symbol or a key word. Supplied as simple, ready-to-use particles that can be added directly to cells, AMSBIO lentivirus eliminates the need for any transfection reagents. Prepared using proprietary protocols to integrate a real-time fluorescence monitoring tag in the system, each 200ul...

AMSBIO has launched DNA-In® CRISPR – an innovative transfection reagent that simplifies and accelerates genome editing using large plasmids and difficult-to-transfect cells for life science researchers generating disease models.

Since its emergence, CRISPR-Cas9 technology has been rapidly adopted as a mainstream approach for performing genetic manipulation. This powerful tool has transformed the process of generating “footprint-free” cellular models for studying genetic diseases. Using this approach, researchers can easily generate disease-relevant isogenic models to determine the impact of correcting or introducing disease-relevant mutations on cellular...

AMSBIO offers a comprehensive range of more than 54,000 lentivirus for shRNA delivery.

To make selection of the optimum lentivirus quick and easy, AMSBIO has developed an online search tool where you need simply enter your target gene symbol or a key word. Lentivirus is a subfamily of the retrovirus family. Lentiviruses can deliver significant amounts of genetic information into host cells and integrate it into the cellular genome. Genetically engineered lentiviruses are therefore used as one of the most efficient tools...

AMSBIO has announced the introduction of a range of Recombinant Prokaryotic Lectins (RPLs).

RPLs are enhanced glycoselective bioaffinity proteins that enable efficient detection, analysis and selective isolation of glycosylated biomolecules. RPLs offer many advantages over traditional plant and eukaryotic lectins. They also offer many advantages over current glycoanalytical and chromatographic methodologies that are not glycoselective or require prior release of glycans which results in the sample destruction. Recombinant...

AMSBIO has launched a  unique series of monoclonal and polyclonal antibodies against S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) that allows immunoassays to be used in methylation regulation research. 

Methylation is involved in nearly every aspect of life. SAM is the sole methyl donor for a variety of methyl-transfer reactions in DNA, RNA, proteins, lipids, phosphilipids, hormones and neurotransmitters. Proper DNA methylation is essential for normal embryonic development. However, when the methyl-transferase gene is homozygously deleted, it is lethal. DNA improperly methylated has been found in many tumors. Alterations in...

AMSBIO has announced a new PD-1 (Programmed Death) inhibitor screening assay kit for cancer research studies.

Immunotherapy for the treatment of cancer is rapidly evolving from therapies that globally and non-specifically simulate the immune system to more targeted activation of individual components of the immune system. The net result of this targeted approach is decreased toxicity and increased efficacy of immunotherapy. The understanding of the checkpoint signalling pathway involving the programmed death 1 (PD-1) receptor and its ligands (PD-L1/2) in...

AMSBIO has announced the launch of Mimetix® 3D cell culture scaffolds that offer significant advantages for a range of applications including regenerative medicine, oncology research, drug discovery, and as a unique aligned scaffold for neural growth and investigation.

There is a definite need for more predictive in vitro efficacy assays to reduce both the number of costly drug failures in clinical trials and the number of animals used in pre-clinical testing. Performing certain cell-based assays in 3D has proven to improve their relevance as models; however, when choosing support for the cell culture growth, important factors to consider are: reproducibility of the environment, similarity to the real-life conditions, ease of...

AMSBIO has introduced an expanded range of Adeno-associated virus (AAV) cloning and packaging services.

Adeno-associated viruses are single-stranded DNA viruses that can infect a broad range of cell types including dividing and non-dividing cells and are, therefore, widely used vehicles for gene delivery. Clinical trials have not only found AAV to be consistently safe and stable, but their vectors are capable of causing long-term expression in human cells. Therefore, AAV vectors have many advantages that many other vectors do not. To date, recombinant AAV...

AMSBIO announces the launch of CellMax™ - a new range of high quality, consistent and affordable cell line FFPE slides.

Made using a patent-pending process, CellMax™ Cell Pellet Slides have a highly consistent density and homogeneity across the entire cell pellet block that ensures you get accurate results time after time. Cells are harvested by a proprietary trypsin-free method preserving cell surface antigens and inflicting minimal physical damage that could lead to a loss of biomarkers. CellMax™ slides produce accurate and reproducible results for immunohistochemistry...

AMSBIO announces the launch of CellMax™ - a new range of high quality, consistent and affordable cell line FFPE slides.

Made using a patent-pending process, CellMax™ Cell Pellet Slides have a highly consistent density and homogeneity across the entire cell pellet block that ensures you get accurate results time after time. Cells are harvested by a proprietary trypsin-free method preserving cell surface antigens and inflicting minimal physical damage that could lead to a loss of biomarkers. CellMax™ slides produce accurate and reproducible results...

AMSBIO announces that Belgian researchers** have cited use of BioPORTER Protein Delivery Reagent to introduce Tau seeds into HEK293 cells.

BioPORTER Protein Delivery Reagent is a unique lipid formulation that allows direct translocation of proteins into living cells. Neurodegenerative tauopathies, including Alzheimer disease and frontotemporal dementias, are characterized by neurofibrillary tangles (NFT) composed of intracellular hyperphosphorylated Tau aggregates. Predominantly expressed in neurons, Tau is a microtubule (MT)-binding protein that stabilizes and promotes the assembly...

AMSBIO announces that Belgian researchers have cited use of BioPORTER Protein Delivery Reagent to introduce Tau seeds into HEK293 cells.

BioPORTER Protein Delivery Reagent is a unique lipid formulation that allows direct translocation of proteins into living cells. Neurodegenerative tauopathies, including Alzheimer disease and frontotemporal dementias, are characterized by neurofibrillary tangles (NFT) composed of intracellular hyperphosphorylated Tau aggregates. Predominantly expressed in neurons, Tau is a microtubule (MT)-binding protein that stabilizes and promotes the...

AMSBIO has launched a new range of human exosomes to the regenerative medicine tools and technologies market.

Derived from placental and adipose derived stem cells, to ensure consistent high quality, these new products are ideally suited for research involving wound healing, stem cell differentiation and tissue regeneration. Exosomes are small vesicles (30–150 nm) containing sophisticated RNA and protein cargos. They are secreted by all cell types in culture and are found to occur naturally in body fluids, including blood, saliva, urine, CSF, and breast milk. The precise molecular mechanics for...

AMSBIO has announced the launch of GMP grade STEM-CELLBANKER® manufactured in strict compliance with Japanese, EU, US, and PICS good manufacturing practice guidelines.

Supplied as a ready-to-use formulation, STEM-CELLBANKER is a chemically defined freezing solution optimised for stem cell and iPS cell storage as well as preservation of other valuable cells. Recent data** supports its unmatched ability for use in cryopreservation of tissues. It is completely free of serum and animal derived component, and contains only US Pharmacopeia, European Pharmacopeia or Japanese Pharmacopeia graded ingredients...

AMSBIO reports on a study, published in Cell, that demonstrates the power of organoids to capture, in three dimensions, the multiple mutations that occur in tumours.

Organoids, small clusters of cells that accurately mimic the behaviour of human tissue, can be used to test cancer drugs and, eventually, to identify effective personalised treatments for patients. In the described research samples from healthy tissue and cancerous tissue were taken from 20 patients with colorectal carcinoma and used to form the organoids. The organoids were then exposed to a range of colorectal cancer treatments as part of a...

Available from AMSBIO TrueORF® cDNA clones are tagged cDNA clones for protein studies.

The TrueORF product line is a new generation of cDNA clone products that enables expression of the encoded transcript as a tagged protein. This facilitates multiple downstream applications that utilize an anti-tag antibody (Myc-DDK or GFP) including protein detection, protein purification, subcellular localization, and others. TrueORFs not only provide an instant solution for tagged protein expression but also offer the flexibility that they...

AMSBIO reports on a study, published in Cell (1), that demonstrates the power of organoids to capture, in three dimensions, the multiple mutations that occur in tumours.

Organoids, small clusters of cells that accurately mimic the behaviour of human tissue, can be used to test cancer drugs and, eventually, to identify effective personalised treatments for patients. In the described research samples from healthy tissue and cancerous tissue were taken from 20 patients with colorectal carcinoma and used to form the organoids. The organoids were then exposed to a range of colorectal cancer treatments as part of a...

AMSBIO has launched a complete new range of commonly used human cytokines and growth factors.

Unlike most of other commercially available cytokine products, ActiveMax® cytokines are expressed exclusively in human cells under strict animal-free and xeno-free conditions. Therefore, they are uniquely suitable for human cell culture and assay development. Endotoxin and other non–human components can provoke immune response in human systems. These contaminations are significant complicating factors for human cell culture experiments, in particular...

AMSBIO has launched a new and exclusive biotinylated protein collection specially designed to simplify your research. 

Attaching biotin to proteins and other macromolecules is an established labelling method which allows specific detection of biotin-tagged molecules via anti-biotin antibody, avidin or streptavidin based reporter systems. Biotinylation reagents are available for targeting specific functional groups or residues, including primary amines, sulfhydryls, carboxyls and carbohydrates. Photoreactive biotin compounds that react nonspecifically...

AMSBIO has launched a new and exclusive biotinylated protein collection specially designed to simplify your research. 

Attaching biotin to proteins and other macromolecules is an established labelling method which allows specific detection of biotin-tagged molecules via anti-biotin antibody, avidin or streptavidin based reporter systems. Biotinylation reagents are available for targeting specific functional groups or residues, including primary amines, sulfhydryls, carboxyls and carbohydrates. Photoreactive biotin compounds that react...

AMSBIO has launched, at the ‘Frontiers in Stem Cells & Cancer' EMBL-EMBO Symposium, a guide that illustrates a variety of organoids grown from different tissues by leading international research groups using BME 2 reduced growth factor extracellular matrix that is available from the company.

Organoids are organ-like structures that can be formed by 3D cell culture and differentiation of stem cells or organ progenitors. Organoids are capable of recapitulating aspects of organ function in in vitro. The current research and therapeutic potential of organoids include organogenesis models, drug testing, tumor / disease models, toxicity screening, personalised medicine and regenerative medicine / organ replacement. BME 2 reduced growth...

AMSBIO has announced a new range of purified, soluble immunoreceptors involved in key signalling pathways. 

Together with new indoleamine 2,3-dioxygenase (IDO) assay kits  these products that can be used to screen for inhibitors of protein-protein interaction, as well as neutralizing antibodies that serve as positive controls for inhibition. Immune evasion is one of the identifying hallmarks of cancer, and researchers are trying to identify the complex mechanisms that enable cancer cells to evade the host’s immune system.  Cancer cells use the...

AMSBIO announces the launch of mRNA-In™ and mRNA-In™ Neuro – a new range of high efficiency, targeted transfection reagents.

Offering high transfection efficiency and expression levels across a broad range of cell types, including stem cells and primary cells, mRNA-In™ is a robust and easy-to-use mRNA delivery solution proven in applications including protein expression and iPSC-reprogramming.  Recognized as among the most difficult cell types to transfect, either freshly isolated or cryopreserved neurons or neural stem cells can be transfected with...

AMSBIO announces the launch of mRNA-In™ and mRNA-In™ Neuro – a new range of high efficiency, targeted transfection reagents.

Offering high transfection efficiency and expression levels across a broad range of cell types, including stem cells and primary cells, mRNA-In™ is a robust and easy-to-use mRNA delivery solution proven in applications including protein expression and iPSC-reprogramming.  Recognized as among the most difficult cell types to transfect, either freshly isolated or cryopreserved neurons or neural stem cells can be transfected with...

AMSBIO has introduced a unique High Throughput PARP in vivo Pharmacodynamic Assay that can be used to monitor the efficacy of poly-ADP-ribose polymerase (PARP) inhibitors on PAR formation in vivo; and to measure drug action on PARP in both in vivo and in vitro settings

In recent news, AstraZeneca reported upon how their ovarian cancer drug Lynparza™ (olaparib) is the first PARP inhibitor to be granted approval by the European Commission and the US Food and Drug Administration (FDA)**. This has led to a surge of interest in this class of drugs, which can exploit tumour DNA repair pathway deficiencies to preferentially kill cancer cells. Dr William Hadlington-Booth of AMSBIO commented "Our High Throughput PARP in vivo...

AMSBIO reports on the recent publication in Cell [1] by Dr Meritxell Huch, Prof Hans Clevers et al. of ground-breaking research using Cultrex BME2 reduced growth factor (organoid growth matrix) to enable long-term (>1 year) culture of genome-stable bipotent stem cells from adult human liver.

These results open up new experimental avenues towards the use of human liver material expanded in vitro as an alternative cell source for disease modeling, toxicology studies, drug testing, regenerative medicine and gene therapy. Liver diseases (ranging from genetic inherited disorders to viral hepatitis, liver cancer, and obesity-related fatty liver disease) are the twelfth-leading cause of death in the United States. Failure in the management of...

AMSBIO has announced the availability of ultraspecific and extensively validated monoclonal antibodies under the UltraMAB™ brand.

Screening of candidate antibodies against greater than 10,000 human proteins, using a state-of-the-art High Density Protein Microarray Platform, has resulted in the identification of the most specific monoclonal antibodies (UltraMAB antibodies) available for cancer biomarkers and other important diagnostic targets. Performance and specificity are key pre-requisites for antibodies to be used for diagnostic and therapeutic applications. To ensure...

AMSBIO announces DNA-In™ Neuro - a new generation transfection reagent developed specifically for maximum nucleic acid delivery into neurons, typically achieving a 2-fold or greater improvement in efficiency over currently available competing reagents.

Neurons are among the most difficult cell types to transfect. DNA-In™ Neuro Transfection Reagent offers researchers a cost effective, robust and easy-to-use DNA delivery solution for transfecting freshly isolated and cryopreserved neurons. DNA-In™ Neuro Transfection Reagent reproducibly transfects neurons and neural stem cells at optimal efficiency with exceptionally low toxicity to support uncompromised post-transfection assays...

AMSBIO has announced several products suitable for the development of an ELISA detection assay for the Ebola virus.

The new products comprise 3 different monoclonal antibodies to the Ebola virus and 2 recombinant proteins (from the Sudan and Zaire strains) suitable for use in producing an optimised ELISA test kit for Ebola. An antigen-capture enzyme-linked immunosorbant assay (ELISA) test for Ebola offers the possibility of diagnosis within the first few days after symptoms begin. Ebola, also known as Ebola hemorrhagic fever, is a disease that typically...

AMSBIO announces MagSi-Direct - a revolutionary technology that brings the power, simplicity, and convenience of magnetic separation to researchers involved in cell biology, protein chemistry, flow cytometry, diagnostics development and many other fields.

A new application note from AMSBIO, previously presented at both the 2014 Beatson International Cancer conference and the ELRIG Drug Discovery 2014 meeting in Manchester UK, introduces a more predictive and realistic model for early stage drug screening of cancer therapeutics.

The report shows that incorporating vascular and stromal cells with breast cancer tumor spheroids allows them to more closely mimic the extracellular environment, cellular architecture and behaviour of actual tumors; this triculture promotes growth, invasion and endothelial recruitment. Current 2D models for evaluating breast cancer progression do not provide a comprehensive, physiological approach to modeling the...

Drawing upon over 20 years’ experience - AMSBIO has developed a custom phospho-specific antibody production service that is both reliable and produces high quality product. 

This service includes synthesis of phosphorylated and non-phosphorylated peptides, conjugation to a carrier protein, immunization, serum production and a 2-step affinity purification of the antibodies. To ensure absolute specificity for each phosphorylated amino acid a stringent dot blot testing regime is carried out guaranteeing that for all projects the phospho peptide is preferentially recognized. A complete package of non-phosphorylated and phosphorylated peptides, pre-immune sera and...

AMSBIO announces MagSi-NGSPREP- a magnetic bead based tool that offers an efficient solution for DNA clean-up and size selection in Next Generation Sequencing (NGS)applications.

MagSi-NGSPREP supports all standard DNA clean-up protocols encountered during Next-Gen library preparation, including the classical one-sided and two-sided "solid phase reversible immobilization" (SPRI) size selection protocols. The simple and flexible protocols employed using MagSi-NGSPREP can be adjusted to your specific application and NGS platform. MagSi-NGSPREP can be used manually but is also easy to automate fo...

AMSBIO offers an extensive range of iPSC derived neural cells including normal and Alzheimer's disease neural progenitor cells, and cerebral cortical neurons.

These neural cells are derived using integration-free, fully defined neural induction conditions. AMSBIO neural cells are capable of generating a spectrum of cortical neurons that are electrically active and have the ability to form functional synapses and circuits in vitro. Cerebral cortical neurons are implicated in numerous neurological diseases including Alzheimer's disease, autism, epilepsy and stroke. Despite strong interest in these diseases, high quality human in...

The Ozilla™ from AMSBIO is a new high quality, purpose-built ozone gas generator. 

Measuring just 13 x 11 x 5 inches (32 x 28 x 13 cm), it will fit in most standard laboratory cell culture incubators, air incubators, cell culture hoods, PCR hoods, or any other environment where a sterile atmosphere is critical. Following simple operational instructions the Ozilla™ is able to completely eliminate airborne as well as surface contaminants and germs including bacteria, phage, and fungus...

AMSBIO announces the introduction of CRISPR/Cas9 - a full gene editing tool designed to produce a genetically modified cell using any mammalian cell line and targeting any gene.

CRISPR/Cas9 is revolutionizing the field of genomic editing by providing scientists with a powerful tool able to change any gene, in any cell in a highly targeted manner and without introducing foreign DNA. The benefits of CRISPR/Cas9 over previous forms of gene editing, such as TALENs and zinc finger, are that it is much simpler to implement and has higher efficiency at performing bi-allelic gene modifications...

BioPORTER® Protein Delivery Reagent from AMSBIO is a unique lipid formulation that allows direct translocation of proteins into living cells.

BioPORTER is the first and only lipid-based protein delivery system that efficiently transports proteins into living cells. Comprising a unique cationic lipid mixture that interacts non-covalently with the protein, BioPORTER creates a protective vehicle for immediate protein delivery. BioPORTER makes studying protein functionality faster and easier than ever before by bypassing the traditional DNA transfection, transcription, and protein translation processes....

Drawing upon a wealth of experience of working with a wide range of 3D cell culture procedures and formats - AMSBIO has produced an extensive 48-page handbook for research scientists looking to culture cells in more physiologically relevant environments.

Over the past two decades, many researchers have actively sought ways to culture cells in 3D. The conclusion reached by many researchers is that there is no single optimal solution, each cell type needs a different environment and many biological processes may need specific methods to study them. Leading the field in the provision of solutions for 3D Cell Culture, AMSBIO’s scientists have distilled the best technologies into a useful guide....

AMSBIO announces ClioCell™ an ex-vivo device for removal of dying and dead cells, improving viability and quality of cell populations and their subsequent productivity.

AMSBIO Lipidure®-Coat low adhesion plates and dishes are shown in cited research to be one of the most effective tools for state-of-the-art 3D spheroid cell culture.  These new plates and dishes are available in 384-well format optimised for drug screening or other high throughput applications.

Spheroid cell culture is based on the spontaneous formation of an aggregation of cells in an environment where the interactions between cells are superior to the interactions between cells and substrate. This can be achieved by using low-adhesion cell culture conditions. Lipidure®-Coat surfaces use MPC (2-methacryloyloxtethyl phosphorylcholine), a biocompatible synthetic polymer with the same structure as the phosphorylcholine polar bases that form cell membranes. As a consequence of the high biocompatibility of this coating, cells will not adhere to the surface of Lipidure®-Coat plates or dishes...

A new range of animal-free and endotoxin-free growth factors and cytokines, tailored to stem cell research, has been launched by AMSBIO – a leading supplier of recombinant proteins.

The success of stem cell research depends on the development of optimal cell culture systems. Both expansion and differentiation of stem cells is highly reliant on growth factor and cytokine supplements, with the activity and purity of these recombinants being critical to successful research and clinical applications....

Presented by Dr Elad Katz, a senior scientist at AMSBIO, a new on-demand webinar explores the potential of 3D cell-based models for regenerative medicine and drug discovery.

Two-dimensional (2D) cell cultures have provided a number of breakthroughs for understanding human tissues and diseases, as well as for discovering and testing new drugs. However, 2D cell cultures have limitations, such as limited cell-cell and cell-matrix contacts, flat cellular morphologies and a lack of realistic mass transfer gradients. As a result, technologies that facilitate growing cells in three-dimensional (3D) configurations have been developed to make cellular behaviors in vitro better resemble the body...

Developed as an alternative to B27® * - GS21 from AMSBIO is a next generation serum-free neural media supplement, based on the formulation of NS21 Supplement, designed to improve the overall growth and performance of primary neurons.

GS21 has been specifically optimized for the maturation and long-term viability of primary rat and mouse neurons in culture, without the need for co-culture with astrocyte feeder cells. In experimental tests, GS21 has been shown to significantly improve long-term viability of rat and mouse primary neurons in vitro, as well as enhance neurite outgrowth of primary neurons in culture. Beneficially, GS21 also supports growth and maintenance of primary neurons at low or high cell density plating...

AMSBIO has announced a new on-demand webinar entitled 'CometAssay: Detecting single and double strand DNA breaks in individual cells'. 

Detecting single and double strand DNA breaks in individual cells'. Presented by Dr Sandra Woodgate, a Subject Matter Expert for this technique., this informative webinar explores how the Comet Assay quantitates DNA damage and repair from in vivo and in vitro samples of eukaryotic cells. The comet assay or single cell gel electrophoresis (SCGE) is a technique to easily and rapidly detect and quantitate DNA fragmentation associated with DNA damage caused by apoptosis and cytotoxicity....

AMSBIO has announced a new on-demand webinar entitled 'CometAssay: Detecting single and double strand DNA breaks in individual cells'.

Presented by Dr Sandra Woodgate, a Subject Matter Expert for this technique., this informative webinar explores how the Comet Assay quantitates DNA damage and repair from in vivo and in vitro samples of eukaryotic cells. The comet assay or single cell gel electrophoresis (SCGE) is a technique to easily and rapidly detect and quantitate DNA fragmentation associated with DNA damage caused by apoptosis and cytotoxicity. The technique can be particularly useful in the field of genotoxicity testing, human biomonitoring, cellular response to DNA damage, and for screening cancer therapeutics...

AMSBIO has announced addition of new biotinylated histones and nucleosomes to its expanding range of recombinant nucleosomes, purified nucleosomes and full length histone proteins.

A nucleosome is the basic unit of DNA packaging. It consists of a segment of DNA wound around a core (“octamer”) of 8 histone proteins (two each of histones H2A, H2B, H3, and H4). The “tails” of these histone proteins stick out, where they can be modified by a number of different histone acetyltransferases, methyltransferases, PARPs, and other epigenetic enzymes. Many of these enzymes modify very specific sites e.g. EZH2, specifically tri-methylates the lysine at position 27 in the H3 histone...

AMSBIO has announced that it has reached agreement with Sanguine BioSciences, abiotechnology company enabling personalized medicine research, to distribute and support its products and services throughout Europe.

Commenting on the new agreement Phillip Pridham-Field of AMSBIO said "Sanguine's approach to biospecimen collection and their high patient retention allow researchers to collect the data they need with better turnaround time and with the potential for longitudinal studies. We believe their extensive biospecimen library will be an excellent addition to our current product offerings for researchers in life sciences...

AMSBIO, a provider of cutting-edge life science technologies and services for accelerating discovery in cancer research, has continued its sponsorship of the annual poster prize at the Beatson International Cancer Conference (7th -10th July 2013).

MagSi-DNA cleanFIX from AMSBIO offers a cost effective solution for purifying DNA fragments, removing all unwanted side products and reagents, such as single nucleotides, terminator dyes, primers and primer dimers, enzymes, buffers and salts.

In many genomics applications, purification of DNA fragments is required to prevent downstream interference with analysis methods. Purification of PCR reactions often requires removal of dNTPs, primers, enzymes, salt by-products and various additives. Historically purification protocols have involved different sample types, different volumes and often multiple washing and centrifugation steps. Using such methods the errors incurred during sample preparation have resulted in lost time and significant costs due to re-work and lost reagents...

AMSBIO offers a range of kits and reagents for the extraction (isolation) of high quality RNA from FFPE tissues and LCM samples that can be used not only for PCR but also microarray applications.

Using AMSBIO ExpressArt® RNA isolation technology it is now possible to achieve high RNA yield and maximum sequence length from FFPE or LCM samples without the paraffin removal step. ExpressArt® RNA technology overcomes traditional limitations placed on research due to limited sample size, RNA degradation and interference from the crosslinking in FFPE samples. ExpressArt® FFPE RNA-ready isolation and mRNA amplification technology allows researcher to isolate higher quality and quantities of RNA from samples previously unsuitable for gene expression analysis...

AMSBIO has announced further expansion of its tissue biorepository and collection services to now include blood products from human donors with auto-immune and neurological conditions.

Whole blood, plasma, serum, PBMCs and cerebrospinal fluid samples are available from a wide variety of donors.  In addition AMSBIO is now able to offer collection services of patient samples that meet the demographic and medical history requirements of specific research studies. Drawing upon over 20 years of serving this market - AMSBIO has established one of the most comprehensive ranges of tissue products to advance personalised medicine by putting these valuable samples in the hands of leading researchers...

AMSBIO has announced a new range of Aptamer Protein Precipitation Kits that bind with unmatched high specificity and affinity to target molecules.

The new AMSBIO Aptoprecipitation (AP) / Co-aptoprecipitation (Co-AP) Kits are aptamer based protein precipitation kits designed to provide easy, quick and consistent protein precipitation. The new kits offer a range of unique characteristics and provide significant advantages over alternative methods. Based upon a proprietary oligonucleotide - Aptamer kits will not contribute to protein/peptide background that can interfere with subsequent mass analysis...

STEM CELLBANKER® from AMSBIO is a chemically defined freezing medium with optimised formulation for stem cells and iPS cells storage as well as other valuable cells.

In a recent study to determine the best cryopreservation solution to maintain induced pluripotent stem (iPS) cells, a team of researchers from the Okayama University Graduate School of Medicine (Japan) compared 12 kinds of commercially prepared cryopreservation solutions and found that STEM CELLBANKER** outperformed the other 11 solutions. The researchers concluded that STEM CELLBANKER** showed the highest cell viability and proliferation of all the solutions tested and can be widely used as it does not require any special skills to use...

AMSBIO has introduced a new range of 96-well format 3D Spheroid Cell Proliferation / Viability Assays, providing a new tool to allow cell-based assays to be carried out in 3D.

The new AMSBIO 3D Spheroid Proliferation/Viability Assay provides a useful tool for modeling tumor response in vitro. The kit utilises a 3D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialised Spheroid Formation Extracellular Matrix to drive aggregation and/or spheroid formation of cells. Upon completion of spheroid formation, the spheroid may be treated with pharmacological agents to evaluate tumor viability after drug treatment...

AMSBIO offers a proven service for synthesis of custom-designed, long mRNA and highly modified mRNAs.

Working from customer supplied target sequence data - AMSBIO has the experience, expertise and facilities to provide a complete, high quality mRNA custom design service. Synthetic mRNA from AMSBIO is supplied highly purified and ready-to-use. Following chemical synthesis of the synthetic DNA, the dsDNA fragments are cloned in a plasmid, purified and full sequencing documentation produced. PCR amplification of the cloned inserts with the primers is then undertaken, purified using spin columns and the resultant DNA template characterised by capillary electrophoresis...

A new informative booklet entitled ‘Short Guide to 3D Cell Culture’ has been produced by AMSBIO

A new white paper, available from AMSBIO, discusses the latest developments in extracellular matrix (ECM) and the opportunities they offer in cancer research

AMSBIO has announced a new 5-Clone Collagen Antibody-Induced Arthritis (CIAA) kit for the induction of Collagen-Induced Arthritis (CIA) in various species

AMSBIO has put together a comprehensive range of genomic DNA and cDNA products derived from various human normal and disease state tissue types. These purified samples save valuable research time allowing the immediate identification of genes of interest without the need to acquire tissues and perform in-house processing

AMSBIO has announced the launch of the 3D Culture 96-Well BME Cell Invasion Assay to address the growing need for more complete and physiologically predictive cancer invasion models

AMSBIO is a leading supplier of innovative tools for growing cells in a physiologically relevant manner. A unique Extracellular Matrix (ECM) Mimetic Library for engineering surfaces to direct receptor binding specificity, signalling and growing cells in 3D has been launched

AMSBIO has announced its new Trilencer-27 siRNA kit, that contains Dicer-Substrate duplexes, provides critical improvements over the use of traditional 21mer siRNA designs

Specialist in high purity reagents for glycobiology and glycoanalysis - AMSBIO - has announced a number of new and unique kits for proteoglycan research.