AMSBIO has announced a new second generation PARP in vivo Pharmacodynamic Assay that accurately measures net poly (ADP-ribose) (PAR) levels in cellular extracts and has been used to document differences in PAR levels in human tumour lysates from a variety of tissues, organs and xenografts. 

Poly-ADP-ribose polymerase (PARP) is a protein involved in a number of cellular processes including catalysing the NAD-dependent addition of PAR onto itself and other adjacent nuclear proteins.  PARP-1 is widely regarded as a promising target for the development of drugs useful in various regimes of cancer therapy, inflammation, ischemia and neurodegeneration. More recently the discovery that breast cancers deficient in homologous recombination are sensitive to non-toxic PARP inhibitors has resulted in efforts by numerous pharmaceutical companies to develop PARP-1 specific drugs.

AMSBIO's PARP in vivo Pharmacodynamic Assay uses a validated sample processing regime and a chemiluminescent, sandwich ELISA format, with pre-coated 96-stripwell plates. The Assay reports PAR level with high signal to noise ratio; sensitivity of 2 pg/ml and a linear dynamic range to 1000 pg/ml.  Consequently the PARP in vivo Pharmacodynamic Assay offers an exciting new tool that provides evidence of drug action on  molecular targets and generates baseline values that may be used to stratify patient response to therapy.

Further information on the PARP in vivo Pharmacodynamic Assay is available at www.amsbio.com/parp-in-vivo-pharmacodynamic-assay.aspx or by contacting AMSBIO on tel. +44-1235-828200. For information detailing AMSBIO's complete range of PARP assay kits, enzymes and antibodies is available at www.amsbio.com/brochures/PAR_Bro_AMS.pdf.

Founded in 1987, AMS Biotechnology (AMSBIO) is recognized as a leading international provider of unique & innovative products & custom services for life sciences research. The amsbio range includes over 23,000 polyclonal & monoclonal antibodies, peptides, recombinant proteins, extracellular matrix, molecular detection reagents, & tissue DNA, RNA, protein & microarray products. Key research areas include: apoptosis, cell invasion & migration, cell signaling, DNA damage, electrophoresis, glycobiology, posttranslational modification & stem cell biology.