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Custom designed long and highly modified mRNAs

Modified mRNAs as templates for in vitro translation

AMSBIO offers a proven service for synthesis of custom-designed, long mRNA and highly modified mRNAs.

Working from customer supplied target sequence data - AMSBIO has the experience, expertise and facilities to provide a complete, high quality mRNA custom design service. Synthetic mRNA from AMSBIO is supplied highly purified and ready-to-use.

Following chemical synthesis of the synthetic DNA, the dsDNA fragments are cloned in a plasmid, purified and full sequencing documentation produced. PCR amplification of the cloned inserts with the primers is then undertaken, purified using spin columns and the resultant DNA template characterised by capillary electrophoresis. The subsequent RNA synthesis uses proven in vitro transcription methodologies and the resultant custom designed mRNA product is then highly purified and fully characterised ready for your use.

While long mRNA can be used as a standard, internal control and for in vitro diagnostics, synthetic modified mRNA is the most advanced and safest method for deriving iPS cells. AMSBIO now offers a non-integrating strategy for reprogramming stem cell fate based on the administration of synthetic modified mRNA that greatly increases efficiency whilst reducing the innate antiviral response of viral protocols.

Additionally modified mRNAs can be used as templates for in vitro translation. ?-containing mRNA is translated more efficiently than its unmodified counterpart, which results in higher protein expression. In addition to the well-known benefit of 5‘-capped mRNAs for mammalian in vitro translation systems, the internal base modifications also have significant effects.

Founded in 1987, AMS Biotechnology (AMSBIO) is recognized today as a leading company contributing to the acceleration of discovery through the provision of cutting-edge life science technology products and services for research and development in the medical, nutrition, cosmetics and energy industries. The AMSBIO range includes specialist antibodies, peptides and recombinant proteins. In addition the company is able to draw upon in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion and proliferation. Widely acknowledged as experts in cell culture, AMSBIO partners with clients in tailoring cell systems to enhance screening outcomes and eventual prognosis. With a range of molecular detection reagents, and a significant Biorepository the company can also provide tissue DNA, RNA, protein and microarray products. Key research areas for these products include: Oncology, Regenerative Medicine, Environmental Analysis, Cytotoxicity Screening, Glycomics and Stem Cell Biology.


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