Channels

 

Special Offers & Promotions

 

 

Latest News

 

 

View Channel

New Products

 

 

View Channel

Video Presentations

 

 

View Channel

Separation Science

 

 

View Channel

Microscopy & Image Analysis

 

 

View Channel

Laboratory Automation & IT Solutions

 

 

View Channel

 

Sensitive detection of cell culture mycoplasma infection

publication date: Oct 16, 2024
 | 
author/source: AMS Biotechnology (Europe) Ltd

sensitive-detection-cell-culture-mycoplasma-infection 

The MycoScope™ Mycoplasma PCR detection kit from AMSBIO uses polymerase chain reaction (PCR) technology to detect mycoplasma infections in less than 3 hours. 

Offering high-sensitivity detection, rapid results, and compatibility with existing PCR setups, this kit reduces the risk of contamination spread saving valuable time and resources.

As reported in the ISSCR standards report (2023) a sizeable proportion of cell cultures (up to >80%) are infected with mycoplasma. While contaminating mycoplasma may grow slowly without killing the cells, the infection affects both cell structure and function rendering any results obtained from infected cultures as unreliable. Testing for mycoplasma is a vital quality control measure used in many cell culture labs.

Though agar cultures as well as DNA fluorochrome staining methods were traditionally used for mycoplasma detection, PCR is rapidly becoming the preferred method of choice due to its high-sensitivity detection.

Evaluated for use with a wide variety of commercially available DNA polymerases, the extremely sensitive MycoScope™ Mycoplasma PCR detection kit can detect less than 5 mycoplasma genomes per microliter of sample. As the MycoScope™ kit primer set is specific to the 16S rRNA coding region in the mycoplasma genome, this allows detection of all mycoplasma species commonly encountered in cell culture.

The rapid detection kit also provides the option of direct testing of cell supernatants without initial DNA extraction to save time and effort. The distinct banding at 500bp on an agarose gel allows easy detection using the same MycoScope™ PCR primer set allowing you to run multiple samples at once. MycoScope™ kits are available in different convenient formats including 100 reaction and 50 reaction sizes.

 

About AMS Biotechnology

Founded in 1987, AMS Biotechnology (AMSBIO) is recognized today as a leading transatlantic company contributing to the acceleration of discovery through the provision of cutting-edge life science technology, products, and services for R&D in the medical, nutrition, cosmetics, and energy industries. AMSBIO has in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion, and proliferation. This expertise in cell culture and the ECM allows AMSBIO to partner with clients in tailoring cell systems to enhance organoid and spheroid screening outcomes using a variety of 3D culture systems, including organ-on-a-chip microfluidics. For drug discovery research, AMSBIO offers assays, recombinant proteins, and cell lines. Drawing upon a huge and comprehensive biorepository, AMSBIO is widely recognized as a leading provider of high-quality tissue specimens (including custom procurement) from both human and animal tissues. The company provides unique clinical grade products for stem cells and cell therapy applications. This includes GMP cryopreservation technology, and high-quality solutions for viral delivery.

 

Recent news from AMSBIO

 


If you have not logged into the website then please enter your details below.



 

News Channels

 

 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month

Top 10 most popular articles this month

 

 

Today's Picks

 

 

 

 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


 

Media Partners

 

Exhibitions & Events