Recent Articles in Separation science - Articles and Application notes
Explaining molecular sizing using GPC/SECNov 22, 2010For many applications that include the use of biologically important molecules, such as proteins or polysaccharides, molecular size is an important parameter but its accurate measurement can be challenging. A new application note from Malvern Instruments describes the use of triple detection gel permeation/ size exclusion chromatography (GPC/SEC) using the VIscotek TDAmax system for this purpose.
Read MoreWyatt Technology Instrumentation Used Against Bacteriophage Infections in Dairy ProductionNov 18, 2010Wyatt Technology Corporation, the world leader in absolute macromolecular characterization instrumentation and software, has been chosen by the Biological Macromolecules Joint Research Unit of the Université de Provence and Université de la Méditerranée in Marseille, France, for its instruments to characterize lactococcus lactis in order to eliminate bacteriophage infections in dairy production.
Read MoreDionex Demonstrates Determination of Free and Total Glycerol in Biodiesel SamplesNov 16, 2010Dionex is pleased to announce the release of a new application demonstrating the use of high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) in the determination of glycerol in biodiesel samples. Application Note 255: Determination of Free and Total Glycerol in Biodiesel Samples by HPAE-PAD Chromatography demonstrates an HPAE-PAD method to determine free and total glycerol from aqueous extractions of biodiesel samples and base-hydrolyzed biodiesel samples, respectively.
Read MoreDionex Demonstrates New Methods to Determine Morpholine, Ethanolamine, and Hydrazine in Nuclear Power Plant WastewaterNov 9, 2010Dionex is proud to announce two sensitive and accurate new methods for determination of μg/L concentrations of commonly used corrosion inhibitors in nuclear power plant (NPP) wastewater. Application Note 247: Determination of Morpholine, Ethanolamine, and Hydrazine in Simulated Nuclear Power Plant Wastewater demonstrates one method optimized to separate hydrazine within 16 min and morpholine within 24 min using the IonPac® CS16 column with suppressed conductivity detection and integrated pulsed amperometric detection (IPAD).
Read MoreDionex Demonstrates Simultaneous Determination of Anions and Cations in Saline and Drinking Water MatricesNov 5, 2010Dionex announces a new method that easily resolves common anions and cations present in saline and drinking water in 20 min. Application Note 260: Monitoring Inorganic Anions and Cations During Desalination demonstrates that the IonPac® AS18 and CS12A columns allow sample analysis with minimal sample pretreatment and maintenance during operation.
Read More New Whitepaper - The Analysis of 16 EPA PAHs by GC/MS using Hydrogen Carrier GasNov 1, 2010The aim of this technical paper is to optimize and produce a robust and repeatable method for the analysis of 16 EPA PAHs by GC/MS using generated hydrogen carrier gas, over helium. This yields vastly improved analytical performance with shortened run times, whilst eliminating laboratory hazards associated with high pressure vessel usage, storage and handling.
Read MoreDionex Demonstrates Simplified Determination of 2-Ethylhexanoic Acid in ClavulanateOct 27, 2010Dionex is proud to announce a simplified method for the detection and quantification of 2-ethylhexanoic acid, a potential impurity in potassium clavulanate active pharmaceutical ingredient (API) incorporated during sample purification. Application Note 262: Determination of 2-Ethylhexanoic Acid Impurity in Clavulanate reports results for limit of detection/limit of quantification (LOQ/LOQ), linear calibration range, spike recovery, retention time precision, and peak area precision determinations...
Read MoreDionex Announces EPA Federal Register Approval of Ion Chromatography for Total Cyanide AnalysisOct 26, 2010The United States Environmental Protection Agency (EPA) is proposing to add new and revised methods published by the EPA, voluntary consensus organizations such as ASTM International, and other commercial entities, for use in water quality monitoring. These changes and additional methods will provide increased flexibility to the regulated community and laboratories in their selection of analytical methods for use in Clean Water Act programs
Read MoreThermo Fisher Scientific Announces Reliable Method for Rapid and Accurate Analysis of Trace Elements in HoneyOct 22, 2010Thermo Fisher Scientific Inc., the world leader in serving science, today announced a new robust, reliable method for analysis of trace elements in honey by atomic absorption. The Thermo Scientific iCE 3500 atomic absorption spectrometer offers a unique dual atomizer design which allows automatic, efficient and safe switching between flame and graphite furnace analyses without the need for user intervention or alignment adjustment.
Read MoreDionex Demonstrates Determination of Total Phosphorus in WastewaterOct 20, 2010
Dionex is pleased to announce the release of an application on the determination of total phosphorus in wastewater. Application Note 254: Determination of Total Phosphorus in Wastewater Using Caro's Reagent and Ion Chromatography describes two IC methods, one with simple carbonate/bicarbonate eluent preparation and a second method that uses generated potassium hydroxide eluent and has the high reproducibility of an RFICTM system.
Read MoreDionex Demonstrates Simplified Determination of Vitamin B12 in BeveragesOct 19, 2010
Dionex is proud to announce a simple, fast, and effective new method for determination of trace amounts (sub-ppb concentrations) of vitamin B12 added to beverages. Application Note 256: Determination of Vitamin B12 in Beverages Using On-line SPE Followed by HPLC with UV Detection details use of an on-line solid-phase extraction (SPE) method, followed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection, performed on an UltiMate® 3000 system using the Acclaim® Polar Advantage II column.
Read MoreDionex Demonstrates Simplified Sample Preparation Method for HPAE-PAD AnalysisOct 11, 2010
Dionex announces a new method that simplifies sample pretreatment for small-scale peptide N-glycosidase F (PNGase F) digests that use protein denaturation. Application Update 176: Preparation of Peptide N-Glycosidase F Digests for HPAE-PAD Analysis demonstrates that this new pretreatment method makes samples amenable to high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) oligosaccharide analysis with the CarboPac® PA200 column, thereby making it possible to monitor the oligosaccharide contentº of a glycoprotein.
Read MoreDionex Demonstrates New Method that Meets U.S. Pharmacopeia Criteria for Determination of N-Methylpyrrolidine in CefepimeOct 6, 2010
Dionex is proud to announce a new method that successfully determines N-methylpyrrolidine (NMP), a cefepime decomposition product, using an ICS-2100 system and IonPac® SCS1 column set coupled with nonsuppressed conductivity detection.
Read MoreDionex Demonstrates Separation of Water- and Fat-Soluble Vitamins in One AnalysisOct 5, 2010
Dionex is proud to announce a new method that can successfully analyze samples prepared in 100% aqueous to 100% organic solvent, thereby allowing water- and fat-soluble vitamins to be separated in one analysis. Application Note 252: HPLC Assay of Water-Soluble Vitamins, Fat-Soluble Vitamins, and a Preservative in Dry Syrup Multivitamin Formulation demonstrates that this accurate and fast separation method can save both analysis time and solvent usage.
Read MoreDionex Presents an IC-MS Technique for Metabolomics StudiesOct 1, 2010
Dionex is pleased to announce the release of a Customer Application Note (CAN) demonstrating the use of ion-exchange chromatography coupled with mass spectrometry for the separation for polar compounds present in biological samples. CAN 108: Ion Chromatography Coupled with Mass Spectrometry for Metabolomics demonstrates efficient separation of biological metabolites using ion chromatography with MS detection.
Read MoreDionex Extends Analysis Methodology for Lithium-Containing Boric Acid-Treated Nuclear Power Plant WatersSep 30, 2010
Dionex is proud to announce that the large-volume, direct-injection approach for the determination of selected organic acids and inorganic anions in borated power plant waters has been extended to include lithium-containing matrices.Application Update 175: Determination of Organic Acids and Inorganic Anions in Lithium-Containing Boric Acid-Treated Nucleardemonstrates use of a single-pump ion chromatography system without a concentrator column to determine
Read MoreYMC-Triart Prep - Application Note: Separation of structurally similar peptides at pH ? 7Sep 30, 2010
Reversed phase chromatography is undoubtedly the most powerful method for peptide purification. In order to improve the cost effectiveness of industrial scale peptide purifications, YMC has developed a new hybrid silica base and a novel bonding technology to improve process efficiencies and inrease column life time.
Read MoreThermo Fisher Scientific Develops New GC-MS Method to Enable Compliance with Updated Federal Workplace Drug Testing GuidelinesSep 16, 2010
Thermo Fisher Scientific Inc., the world leader in serving science, introduces a new method for the confirmation of amphetamines in urine in accordance with recently updated United States Substance Abuse and Mental Health Services Administration (SAMHSA)
Read MoreNew Biochrom Libra S80 UV-Vis spectrophotometer Sep 8, 2010
Global scientific instrument manufacturer Biochrom is targeting its new UV-Visible spectrophotometer, the Biochrom Libra S80, at customers looking for the flexibility and future proofing that a variable bandwidth optical system offers. Featuring double beam optical design and variable bandwidth (0.5 nm, 1 nm, 2 nm, 4 nm), Biochrom's new "top of the range instrument" is fully EP (European Pharmacopoeia) compliant and is the ideal choice for both research and method development laboratories.
Read MoreSep 8, 2010
A newly available application note from Malvern Instruments explains why, when measuring samples using Gel Permeation Chromatography/Size Exclusion Chromatography (GPC/SEC), low angle light scattering (LALS) is the only LS technique that actually measures a scattered light intensity directly proportional to molecular weight.
Read MoreFree Guide to HPLC formulae available from YMCSep 6, 2010
A free guide to HPLC formulae from YMC is available to download.
Read MoreSage-N ResearchSep 1, 2010
Sage-N Research Inc., (Sage-N) the world leader in computational proteomics announces the integration of the first commercially available phosphorylation tool using mass spectrometry into its drug discovery platform. The new phosphorylation algorithm, based on a method developed by Professor Steve Gygi of Harvard Medical School, measures the likelihood of correct phosphorylation site localization which is important for the study of many diseases like cancer.
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For many applications that include the use of biologically important molecules, such as proteins or polysaccharides, molecular size is an important parameter but its accurate measurement can be challenging. A new application note from Malvern Instruments describes the use of triple detection gel permeation/ size exclusion chromatography (GPC/SEC) using the VIscotek TDAmax system for this purpose.
Wyatt Technology Corporation, the world leader in absolute macromolecular characterization instrumentation and software, has been chosen by the Biological Macromolecules Joint Research Unit of the Université de Provence and Université de la Méditerranée in Marseille, France, for its instruments to characterize lactococcus lactis in order to eliminate bacteriophage infections in dairy production.
Dionex is pleased to announce the release of a new application demonstrating the use of high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) in the determination of glycerol in biodiesel samples. Application Note 255: Determination of Free and Total Glycerol in Biodiesel Samples by HPAE-PAD Chromatography demonstrates an HPAE-PAD method to determine free and total glycerol from aqueous extractions of biodiesel samples and base-hydrolyzed biodiesel samples, respectively.
The aim of this technical paper is to optimize and produce a robust and repeatable method for the analysis of 16 EPA PAHs by GC/MS using generated hydrogen carrier gas, over helium. This yields vastly improved analytical performance with shortened run times, whilst eliminating laboratory hazards associated with high pressure vessel usage, storage and handling.
Thermo Fisher Scientific Inc., the world leader in serving science, today announced a new robust, reliable method for analysis of trace elements in honey by atomic absorption. The Thermo Scientific iCE 3500 atomic absorption spectrometer offers a unique dual atomizer design which allows automatic, efficient and safe switching between flame and graphite furnace analyses without the need for user intervention or alignment adjustment.