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Faster Separation and Quantification of Metabolites in Brassica napus Rapeseeds

DionexDionex is proud to announce a new platform developed by a customer to measure various metabolites in Brassica napus seeds. In cooperation with the Leibniz Institute of Plant Genetics and Crop Plant Research, the company has released Customer Application Note 109: Primary Metabolite Analysis of Plant Material Using a Triple Quadrupole MS Coupled to a Monolith Anion-Exchange Column. This method combines separation using the 1 mm IonSwiftTM MAX-100 anion-exchange column and ICS-3000 system and detection with the API 4000TM electrospray ionization (ESI) tandem mass spectrometry (MS/MS) system from AB SCIEX, providing a simple, rapid, specific, and sensitive method for the simultaneous detection and quantification of several primary metabolites involved in the tricarboxylic acid (TCA) cycle and glycolysis pathway. Compared to the previously used method, the IonSwift MAX-100 column facilitates shorter run times and helps achieve better sensitivity.

This new platform combines Dionex ion chromatography (IC) and triple quadrupole MS. The IC tandem mass spectrometry (IC-MS/MS) technique provides a good tool to identify and quantify metabolites in a complex matrix. The biological pathways include many homologs and structural isomers with the same mass and analog fragmentations, so it is essential for the analysis to provide good resolution and specific identification of the different compounds.

This customer application note and many others can be found at www.dionex.com under the Documents tab.

Dionex (NASDAQ:DNEX) is a global leader in the manufacturing and marketing of liquid chromatography and sample preparation systems, consumables, and software for chemical analysis. The company's systems are used worldwide in environmental analysis and by the life sciences, chemical, petrochemical, food and beverage, power generation, and electronics industries. Our expertise in applications and instrumentation helps analytical scientists to evaluate and develop pharmaceuticals, establish environmental regulations, and produce better industrial products.

IonSwift is a trademark of Dionex Corporation.

API 4000 is a trademark of AB SCIEX.



 

 

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