Thermo Fisher Scientific has developed an on-line solid-phase extraction–high-performance liquid chromatography (SPE-HPLC) method combined with ultraviolet (UV) absorbance detection for the simple and sensitive determination of tigecycline in a cell lysate sample.
Application Note 1074:Determination of Tigecycline in a Cell Lysate demonstrates that this approach, performed on a rapid separation LC (RSLC) system controlled by data management software, detects trace amounts of tigecycline in tigecycline-treated cells, concentrations so low that they cannot be assayed using a routine HPLC-UV detection method. The prevalence of multidrug-resistant bacteria in hospitals underscores the need for...
Thermo Fisher Scientific has developed a high-performance liquid chromatography (LC) method that simplifies determination of clenbuterol, ractopamine, and phenolethanolamine in meat using on-line solid-phase extraction (SPE) and ultraviolet absorbance detection.
Application Brief (AB) 154: Rapid and Sensitive Determination of Clenbuterol, Ractopamine, and Phenolethanolamine in Meatdemonstrates that this method, performed using a dual rapid separation LC system controlled by data management software, achieves method detection limits equivalent to those obtained by mass spectrometry (MS). Additionally, this approach offers the advantages of...
Magritek, a leading worldwide provider of compact NMR and MRI instruments, have released three new applications notes which showcase the use of their Spinsolve Benchtop NMR Spectrometer.
Magritek has released three new applications notes to illustrate the versatility of their Spinsolve family of cost-effective benchtop NMR spectrometry solutions. Spinsolve uses standard 5 mm OD NMR test tubes, exactly the same as high field instruments, so sample handling is familiar and convenient. Spinsolve can be deployed in the lab where immediate results may now be obtained...
Cogent TYPE-C silica LC phases from MicroSolv Technologies have the ability to retain polar solutes at high concentrations of organic solvent by aqueous normal-phase (ANP) and non-polar compounds under reversed-phase (RP) conditions.
These columns use patented bonding technology to create a surface populated by silicon-hydride functional groups instead of silanols. The lack of surface silanols leads to fast equilibration times, excellent peak shape and extended column lifetimes for a wide range of analytes. To demonstrate the unique abilities of Cogent TYPE-C silica LC phases a wide range of application notes are available including.....
Read MoreRapid and Reliable Determination of Phenolic Compounds in SoilMay 19, 2014
Thermo Fisher has developed an efficient and simple high-performance liquid chromatography (HPLC) method with ultraviolet detection for the determination of phenolic compounds (gallic acid, caffeic acid, 4-hydroxybenzoic acid, syringic acid, ferulic acid, salicylic acid, phloroglucinol, cinnamic acid, catechin, quercetin, vanillin, phloridzin, and phloretin) in apple orchard soil.
Application Note (AN) 1077: Determination of Phenolic Compounds in Apple Orchard Soil demonstrates that the entire analysis process, including sample preparation and separation for one soil sample, can be accomplished within 80 minutes using this method combined with an accelerated solvent extractor....
Thermo Fisher Scientific has developed a method for quantitative determination of over 200 multi-residue pesticides present in a blend of herbs and spices known as “churna”and widely used in Ayurvedic medicine using gas chromatography-tandem mass spectrometry (GC-MS/MS).
Application Note 10361, Analysis of Multi-Residue Pesticides Present in Ayurvedic Churna by GC-MS/MS describes a sensitive and precise assay for the detection of pesticides. Trace levels of the multi-residue pesticides were detected in the highly complex churna extracts at concentrations as low as 2.5 ng/g. The analysis was performed using a Thermo Scientific™ TRACE™ 1310 GC coupled to a Thermo Scientific™ TSQ 8000™ triple-quadrupole GC-MS/MS.
Thermo Fisher Scientific has released a new technical note which demonstrates the complete recovery of human hormones from drinking water.
Technical Note 148: Automated Extraction and Determination of Human Hormones in Drinking Water uses our newly released polymeric sorbent extraction cartridges (Thermo Scientific™ Dionex™ SolEx™ SPE HRPHS cartridges) on our solid-phase extraction instrument (Thermo Scientific™ Dionex™ AutoTrace 280 instrument) for the determination of human hormones. The SPE instrument can reduce solvent and labor costs by up to 90% in sample preparation...
Latest Application Note from Wyatt Technology Demonstrates How Absolute Molar Mass can be Measured Using UHPLC SEC-MALS
Wyatt Technology Corporation, the world leader in absolute macromolecular characterization instrumentation and software, has announced the availability of a new application note which outlines the combined use of the Wyatt μDAWN™ multi-angle light scattering (MALS) detector and the Optilab® UT-rEX™ refractive index (RI) detector to measure the absolute molecular weight (or molar mass) and size of eluting species in ultra high performance liquid chromatography (UHPLC)....
Read MoreSimplified Determination of Inositol Phosphates in Dried Distillers Grains with SolublesApr 22, 2014
Thermo Fisher Scientific has developed a rapid and reliable sample preparation procedure and ion chromatography-based method to determine the phytate hydrolysis products in dried distillers grains with solubles (DDGS).
Application Note 1070: Determination of Inositol Phosphates in Dried Distillers Grains with Solubles demonstrates the determination of 25 inositol phosphate isomers—InsP2–InsP6—in an in-house prepared reference solution. Separation is achieved in less than 42 minutes using a column designed for high-resolution separations of carbohydrates. Using this separation, inositol phosphates are determined in DDGS....
Thermo Fisher Scientific has developed an ion chromatography (IC)-based method for the determination of ammonia in adenosine that can easily meet the sensitivity requirements in the U.S. Pharmacopeia (USP) adenosine monograph.
Application Note 1072: Ion Chromatography Assay for Ammonia in Adenosinedemonstrates that this proposed IC method for the determination of ammonia in adenosine enables an analysis time of 5 min, thereby yielding high sample throughput. This IC assay avoids the hazardous chemicals used in the color-based ammonia assay in the USP adenosine monograph...
Thermo Fisher Scientific has developed a reproducible and accurate ion chromatography (IC) method for determining monochloroacetic acid (MCAA) in carbocisteine drug substance and two monochloroacetic acid drug products, thus enabling efficient quality control of the carbocmanufacturing processisteine.
Application Note 1076: Determination of Monochloroacetic Acid in Carbocisteine demonstrates that this IC method is easily executed using a reagent-free IC system controlled by data management software. The analyst simply adds water and samples to separate and accurately determine MCAA using a hydroxide-selective anion-exchange column (Thermo Scientific™ Dionex™ IonPac™ AS11-HC column)...
Bruker has released an application note detailing a simple and convenient method for the quantification of trace pharmaceutical and personal care products (PPCPs) in clean water, using Bruker’s EVOQ triple quadrupole liquid chromatography mass spectrometer (LC-MS/MS).
PPCPs are products used for personal health or cosmetic reasons. PPCPs in environmental and potable water are potentially harmful for both humans and the environment, so are strictly regulated by environmental bodies such as the United States Environmental Protection Agency (US EPA). Bruker’s technique proved simpler than traditional solid phase extraction (SPE) based methods and the EVOQ showed excellent sensitivity and robustness throughout....
Read MoreChromium (VI) in toys Mar 11, 2014
Children are exposed to intakes of heavy metals from a wide variety of sources. Chromium (VI) in particular represents a potential hazard, as it is absorbed from food and drinking water, from the air that is breathed, from textiles, from utensils that contain metal and from toys.
The analytical determination of chromium (VI) content in toys for compliance with limit values is described in the European standard DIN EN-71-3-2013 (Safety of toys Part 3 – Migration of certain elements). The standard differentiates between three categories, depending on the kind of toys...
Thermo Fisher Scientific has developed a new gradient high-performance liquid chromatography methods that use a spectro-electro array platform
Thermo Fisher Scientific has developed a new gradient high-performance liquid chromatography methods that use a spectro-electro array platform to measure specific analytes in beer samples, distinguish between different samples, and study beer stability. Application Note 1065: Gradient HPLC Method for Analysis of Beer Polyphenols, Proanthocyanidins, and Bitter Acids Using a Novel Spectro-Electro Array Platform demonstrates a method that provides accurate and sensitive measurements that cannot be obtained using ultraviolet detection alone, as well as metabolomic approaches that can be used to study fermentation, product stability, and authenticity issues...
Wyatt Technology Corporation, the world leader in absolute macromolecular characterization instrumentation and software, has published a new whitepaper on characterizing molecular structure in synthetic and natural polymers by multi-angle light scattering. Authored by Stepan Podzimek, Scientific Consultant for Wyatt Technology, the paper details the basic principles of the detection and characterization of branching by means of a multi-angle light scattering (MALS) detector and the various methods used...
Thermo Fisher Scientific has developed a rapid and robust high-performance anion-exchange chromatography
With pulsed amperometric detection method for the accurate determination of common sugars in acid-hydrolyzed biomass samples with high carbohydrate concentrations. Application Update 192: Carbohydrate Determination of Biofuel Samples demonstrates that this approach has a linear range suitable for handling high-concentration biomass samples with minimal sample treatment. This new method is fast, provides good sensitivity, has consistent response, and can be routinely used to determine carbohydrates in the feedstock for biofuel production...
Bruker releases application note for analysis of sulfonamides in honey using Bruker’s Advance EVOQ Elite LC-MS/MS system
Bruker Chemical and Applied Markets (CAM), Fremont, California, US: Bruker releases application note for analysis of sulfonamides in honey using Advance EVOQ Eliteruker has released an application note detailing a simple, fast and robust method developed for the quantitation of sulfonamides in honey, using Bruker’s EVOQ Elite triple quadruple liquid chromatography mass spectrometer (LC-MS/MS). Sulfonamides are a group of broad spectrum antibacterial drugs, used to prevent and treat bacterial growth in honey products...
Thermo Fisher Scientific has developed a reproducible and accurate high-performance liquid chromatography (HPLC) method combined with on-line solid-phase extraction for the sensitive determination of carbendazim in orange juice.
Application Note 1067: Determination of Carbendazim in Orange Juice demonstrates that this application—performed using a rapid separation liquid chromatography system—simplifies sample preparation and improves HPLC detection sensitivity with a method detection limit of carbendazim in orange juice of 0.2 μg/L. Although carbendazim is a fungicide used in many countries to improve crop production....
Thermo Fisher Scientific is pleased to announce a new technical note TN 139
Thermo Fisher Scientific is pleased to announce a new technical note TN 139: Determination of Anions in Fracking Flowback Water From the Marcellus Shale Using Automated Dilution and Ion Chromatography which demonstrates the use of in-line conductivity measurement to trigger automated dilution prior to separation and quantification of inorganic ions and organic acids in Marcellus Shale fracking flowback samples using ion chromatography...
New Insights Published on Key Protein Interactions Involved in the Bacterial Flagellar Motor Complex Show Promise for Other Difficult Biomolecular Systems
New Application Note from Wyatt Technology Showcases the Ability of CG-MALS to Quantify Complex Proteins. Wyatt Technology Corporation, the world leader in absolute macromolecular characterization instrumentation and software, has today published a new application note highlighting a study on the complex interactions between proteins and how they modulate the rotational direction of bacteria flagella....
Thermo Fisher Scientific is pleased to announce a new technical note which demonstrates high resolution chromatographic separations of organic acids in beer samples.
Organic acids are end products of yeast fermentation critical to the flavor of beer, but are also products of bacterial fermentation that introduce a sour flavor, either purposely or unintentionally due to spoilage. Technical Note 126: Determination of Organic Acids in Beer Samples Using a High-Pressure Ion Chromatography System specifically discusses the separation of organic acids in beer samples using a Thermo Scientific™ Dionex™ IonPac™ AS11-HC-4μm anion-exchange column....
Malvern Instruments has posted a new application note demonstrating the use of the company’s Viscotek SEC-MALS 20 multi angle light scattering (MALS) detector for size exclusion chromatography (SEC) in the analysis of monoclonal antibodies.
The application note, ‘Molecular weight measurements of an antibody using Viscotek SEC-MALS 20’, reports experimental data for the analysis of aggregates within monoclonal antibody formulations and can be freely downloaded. Monoclonal antibodies are being targeted for use in a growing range of therapeutic applications. Measuring the molecular weight of the protein, monomer, oligomers and aggregates within the sample and in formulations is an important part of assessing their safety and efficacy as drugs.....
Magritek has released three new applications notes to illustrate the versatility of their Spinsolve family of cost-effective benchtop NMR spectrometry solutions. Spinsolve uses standard 5 mm OD NMR test tubes, exactly the same as high field instruments, so sample handling is familiar and convenient. Spinsolve can be deployed in the lab where immediate results may now be obtained...
These columns use patented bonding technology to create a surface populated by silicon-hydride functional groups instead of silanols. The lack of surface silanols leads to fast equilibration times, excellent peak shape and extended column lifetimes for a wide range of analytes. To demonstrate the unique abilities of Cogent TYPE-C silica LC phases a wide range of application notes are available including.....
Application Note 10361, Analysis of Multi-Residue Pesticides Present in Ayurvedic Churna by GC-MS/MS describes a sensitive and precise assay for the detection of pesticides. Trace levels of the multi-residue pesticides were detected in the highly complex churna extracts at concentrations as low as 2.5 ng/g. The analysis was performed using a Thermo Scientific™ TRACE™ 1310 GC coupled to a Thermo Scientific™ TSQ 8000™ triple-quadrupole GC-MS/MS.
Wyatt Technology Corporation, the world leader in absolute macromolecular characterization instrumentation and software, has announced the availability of a new application note which outlines the combined use of the Wyatt μDAWN™ multi-angle light scattering (MALS) detector and the Optilab® UT-rEX™ refractive index (RI) detector to measure the absolute molecular weight (or molar mass) and size of eluting species in ultra high performance liquid chromatography (UHPLC)....
Application Note 1072: Ion Chromatography Assay for Ammonia in Adenosinedemonstrates that this proposed IC method for the determination of ammonia in adenosine enables an analysis time of 5 min, thereby yielding high sample throughput. This IC assay avoids the hazardous chemicals used in the color-based ammonia assay in the USP adenosine monograph...
Application Note 1076: Determination of Monochloroacetic Acid in Carbocisteine demonstrates that this IC method is easily executed using a reagent-free IC system controlled by data management software. The analyst simply adds water and samples to separate and accurately determine MCAA using a hydroxide-selective anion-exchange column (Thermo Scientific™ Dionex™ IonPac™ AS11-HC column)...
The analytical determination of chromium (VI) content in toys for compliance with limit values is described in the European standard DIN EN-71-3-2013 (Safety of toys Part 3 – Migration of certain elements). The standard differentiates between three categories, depending on the kind of toys...
Thermo Fisher Scientific has developed a new gradient high-performance liquid chromatography methods that use a spectro-electro array platform to measure specific analytes in beer samples, distinguish between different samples, and study beer stability. Application Note 1065: Gradient HPLC Method for Analysis of Beer Polyphenols, Proanthocyanidins, and Bitter Acids Using a Novel Spectro-Electro Array Platform demonstrates a method that provides accurate and sensitive measurements that cannot be obtained using ultraviolet detection alone, as well as metabolomic approaches that can be used to study fermentation, product stability, and authenticity issues...
Bruker Chemical and Applied Markets (CAM), Fremont, California, US: Bruker releases application note for analysis of sulfonamides in honey using Advance EVOQ Eliteruker has released an application note detailing a simple, fast and robust method developed for the quantitation of sulfonamides in honey, using Bruker’s EVOQ Elite triple quadruple liquid chromatography mass spectrometer (LC-MS/MS). Sulfonamides are a group of broad spectrum antibacterial drugs, used to prevent and treat bacterial growth in honey products...
