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Tetrahydrocannabinol Metabolite in Urine, Sarcosine and Taurine Analysis

publication date: May 27, 2014
author/source: Hichrom

Cogent TYPE-C silica LC phase

Cogent TYPE-C silica LC phases from MicroSolv Technologies have the ability to retain polar solutes at high concentrations of organic solvent by aqueous normal-phase (ANP) and non-polar compounds under reversed-phase (RP) conditions.

These columns use patented bonding technology to create a surface populated by silicon-hydride functional groups instead of silanols. The lack of surface silanols leads to fast equilibration times, excellent peak shape and extended column lifetimes for a wide range of analytes. To demonstrate the unique abilities of Cogent TYPE-C silica LC phases a wide range of application notes are available including:

Cogent Phenyl Hydride for Analysis of Tetrahydrocannabinol Metabolite in Urine

9-Carboxy-11-nor-delta 9-tetrahydrocannabinol is the main metabolite of tetrahydrocannabinol (THC), formed in the body after consumption of cannabis. The compound stays in the body for a significant time, making it useful as a test compound for cannabis use. Following extraction of this cannabis metabolite from urine by SPE, it was successfully analysed by LC-MS using a Cogent Phenyl Hydride column, based on Type-C silica.

Separation of Sarcosine from Isobaric beta-Alanine

It is difficult to successfully separate sarcosine from isobaric beta-alanine under standard reversed-phase conditions without intensive sample preparation (i.e. derivatisation), as both compounds tend to elute at the solvent front. An alternative simple gradient LC-MS method, using a Cogent Diamond Hydride TYPE-C silica LC column shows the separation of these two compounds without the need for derivatisation. The developed method is sensitive, specific, quantitative and reproducible (%RSD = 0.1).  

LC-MS Analysis of Taurine, a Polar Sulphonate Compound

Taurine is a non-essential sulphur-containing amino acid. It is used in food and pharmaceutical formulations, including many popular energy drinks. It is also found naturally in animal tissues and is a major constituent of bile. The high polarity of taurine creates a challenge for the reversed-phase HPLC of the underivatised compound. Cogent Diamond Hydride columns have been shown to provide excellent retention and peak shape under aqueous normal phase conditions for the LC-MS analysis of taurine.

For copies of these application notes and many others please contact Hichrom Limited at or +44(0)118 930 3660 or alternatively copies of all application notes are available on the MicroSolv web site

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