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Fast and Accurate Isocratic Method to Determine Ammonia in Adenosine

publication date: Apr 18, 2014
 | 
author/source: Thermo Fisher Scientific

thermo fisher

Thermo Fisher Scientific has developed an ion chromatography (IC)-based method for the determination of ammonia in adenosine that can easily meet the sensitivity requirements in the U.S. Pharmacopeia (USP) adenosine monograph.

Application Note 1072: Ion Chromatography Assay for Ammonia in Adenosinedemonstrates that this proposed IC method for the determination of ammonia in adenosine enables an analysis time of 5 min, thereby yielding high sample throughput. This IC assay avoids the hazardous chemicals used in the color-based ammonia assay in the USP adenosine monograph.

This methoduses an IC column with small-diameter resin (Thermo Scientific Dionex IonPac CS-12A-5µm column) to determine ammonia in adenosine. The electrolytically generated eluent and self-regenerating suppressor contribute to the robustness and sensitivity of the method. The precision, accuracy, linearity, detection and quantitation limits, and robustness of the assays used to determine ammonia in adenosine meet USP General Chapter <1225> guidelines for method validation. In addition, only water is used for eluent generation, thus eliminating the need for handling hazardous chemicals.


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About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of $12 billion, we have approximately 39,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through three premier brands, Thermo Scientific, Fisher Scientific and Unity™ Lab Services, which offer a unique combination of innovative technologies, convenient purchasing options and a single solution for laboratory operations management. Our products and services help our customers solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity.


 


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