Thermo Fisher Scientific has developed an ion chromatography (IC) method for determining low concentrations of morpholine in linezolid using an eluent generation system with suppressed conductivity detection.

Morpholine is used in the synthesis of linezolid and may be present in the drug product. This approach achieves efficient separation using a high-performance cation-exchange column with a high-purity electrolytically generated methanesulfonic acid eluent. Application Note 1062: Determination of Morpholine in Linezolid by Ion Chromatography demonstrates that this IC method also allows simultaneous determination of sodium, ammonium, potassium, and other cations present in the sample.

To prevent column contamination by linezolid, this method uses in-line matrix elimination to concentrate trace morpholine on a pellicular cation-exchange concentrator column, followed by removal of the uncharged linezolid with deionized water using a high-precision autosampler and then elution of the morpholine onto the high-performance cation-exchange column.

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of $12 billion, we have approximately 39,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through three premier brands, Thermo Scientific, Fisher Scientific and Unity™ Lab Services, which offer a unique combination of innovative technologies, convenient purchasing options and a single solution for laboratory operations management. Our products and services help our customers solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity.

 more about Eluent Generation Modules

more about Thermo Fisher

more news from Thermo Fisher