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Fast HPAE-PAD Fast Carbohydrate Determination of Biomass Samples

publication date: Feb 18, 2014
 | 
author/source: Thermo Fisher Scientific

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Thermo Fisher Scientific has developed a rapid and robust high-performance anion-exchange chromatography

With pulsed amperometric detection method for the accurate determination of common sugars in acid-hydrolyzed biomass samples with high carbohydrate concentrations. Application Update 192: Carbohydrate Determination of Biofuel Samples demonstrates that this approach has a linear range suitable for handling high-concentration biomass samples with minimal sample treatment. This new method is fast, provides good sensitivity, has consistent response, and can be routinely used to determine carbohydrates in the feedstock for biofuel production.

In order to maximize biofuel yield, the carbohydrates released by acid hydrolysis must be quantified, which requires analysis of a large number of samples. Hence, there is a need for a fast, robust, accurate, and quantitative analytical method for carbohydrate determination of biomass samples. This new approach overcomes key limitations of other available methods. Use of a specially designed anion-exchange column for rapid separation of small carbohydrates with electrolytically generated hydroxide eluent, combined with a smaller sample injection and reduced sensitivity at the point of detection, reduces the degree of sample dilution required and speeds analysis.


more about Application Update 192


About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of $12 billion, we have approximately 39,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through three premier brands, Thermo Scientific, Fisher Scientific and Unity™ Lab Services, which offer a unique combination of innovative technologies, convenient purchasing options and a single solution for laboratory operations management. Our products and services help our customers solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity.


more about Thermo Fisher


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