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Debunking Myths about Peak Area Response of Glycoprotein Oligosaccharides

Thermo Fisher Scientific has released a technical note (TN) that challenges the common misconception that there is significant variation in high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) peak area response of oligosaccharides.

Debunking Myths about Peak Area Response of Glycoprotein OligosaccharidesTN 133: HPAE-PAD Peak Area Response of Glycoprotein Oligosaccharides summarizes the peer-reviewed scientific literature concerning this subject. This review concludes that the variation in HPAE-PAD oligosaccharide response with structure is minimal, especially among sets of similar oligosaccharides such as those found on many glycoproteins.

HPAE-PAD is a key technique used for characterizing recombinant therapeutic glycosylation because it can be used for monosaccharide, sialic acid, and oligosaccharide analyses (Asn-linked [N-linked] and Ser/Thr-linked [O-linked] oligosaccharides)—commonly referred to as oligosaccharide profiling. In this review of scientific literature evaluating electrochemical response of glycoprotein-derived and related oligosaccharides, TN 133 shows that the changes of electrochemical response with structure are not extreme. TN 133 then compares HPAE-PAD to other common methods of oligosaccharide profiling, including matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis of oligosaccharides.


 view technical note 133: HPAE-PAD Peak Area Response of Glycoprotein Oligosaccharides.


 About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of $12 billion, we have approximately 39,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through three premier brands, Thermo Scientific, Fisher Scientific and Unity™ Lab Services, which offer a unique combination of innovative technologies, convenient purchasing options and a single solution for laboratory operations management. Our products and services help our customers solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity


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