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Simplified Determination of Inositol Phosphates in Dried Distillers Grains with Solubles

publication date: Apr 22, 2014
author/source: Thermo Fisher Scientific

thermo fisher chromatography

Thermo Fisher Scientific has developed a rapid and reliable sample preparation procedure and ion chromatography-based method to determine the phytate hydrolysis products in dried distillers grains with solubles (DDGS).

Application Note 1070: Determination of Inositol Phosphates in Dried Distillers Grains with Solubles demonstrates the determination of 25 inositol phosphate isomers—InsP2–InsP6—in an in-house prepared reference solution. Separation is achieved in less than 42 minutes using a column designed for high-resolution separations of carbohydrates. Using this separation, inositol phosphates are determined in DDGS. Sample pretreatment cartridges simplify sample preparation for DDGS compared to typical capture/release methods.

Ins-P6 in its ionic form, phytate, accounts for 50–80% of the phosphorus in seed-based feedstuff. In a complexed form, however, phytate’s phosphorus and bound minerals become nutritionally unavailable. Therefore, DDGS are supplemented with inorganic phosphorus to meet the dietary needs of nonruminant animals. Processing factors such as fermentation, drying, and pelleting contribute to the nutritional variation in DDGS. Thus, this new analytical method to quantify phytate hydrolysis products in DDGS is useful in determining phosphorus bioavailability for nonruminant animals.

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About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of $12 billion, we have approximately 39,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through three premier brands, Thermo Scientific, Fisher Scientific and Unity™ Lab Services, which offer a unique combination of innovative technologies, convenient purchasing options and a single solution for laboratory operations management. Our products and services help our customers solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity.

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