Follow us...

 

Search News Archives

Channels

News

 

 

View Channel

Laboratory Products

 

 

View Channel

Special Offers and Promotions

 

Microscopy | Image Analysis

 

 

View Channel

Separation Science

 

 

View Channel

Coronavirus (COVID-19)

 

 

View Channel

Research & Case Studies

 

 

View Channel

Conferences | Events

Efficient Isolation of High Yield gDNA

Efficient Isolation of High Yield gDNAAgilent Automation Solutions has released an application note that demonstrates how the Agilent gDNA Isolation Workstation, utilizing the Invitrogen ChargeSwitch EasyPlex kit, provides the throughput, and walk-away time necessary to keep up with the demands of genomic applications.

Efficient isolation of high-yield gDNA is an important step in high-throughput analysis of the genome. Isolating gDNA can be accomplished by a number of approaches. Automated liquid-handling workstations can be used to isolate gDNA in a reliable, repeatable, and contamination-free manner. This application note outlines a protocol for the Invitrogen ChargeSwitch EasyPlex gDNA kit using the Agilent gDNA isolation workstation in which up to 10 microplates (96-well) can be processed simultaneously.  The throughput of the outlined protocol is 10 minutes for each plate processed (depending on exact liquid handling procedures).

In the described set-up - microplates and tip boxes were delivered to an Agilent Bravo Automated Liquid Handling Platform by an Agilent BenchCel Microplate Handling. The Bravo gripper was used to transport microplates and tip boxes around the platform. After the washing steps, the microplates were sealed with an Agilent PlateLoc Thermal Microplate Sealer and stored in the BenchCel Workstation for downstream use. The authors show how the Agilent Bravo Automated Liquid Handling Platform has the capacity for managing the necessary reagents, plates, and tips in a compact footprint. Further the integration of three Agilent BenchCel stackers for tip boxes is shown to provide an uninterrupted supply of tips without user intervention. The Agilent PlateLoc Thermal Microplate Sealer is shown to securely seal the plates after processing, and is easily integrated into the process workflow. Agilent VWorks Automation Control software was used to create an event-driven protocol to seamlessly integrate and reliably manage the entire process.

For a copy of the application note please visit www.chem.agilent.com/Library/applications/5990-3549en_lo%20CMS.pdf or contact your local Agilent Automation sales office.

From stand-alone units to fully integrated systems, Agilent Automation Solutions teams key sample preparation and creative walk-away automation approaches with personalized customer service to provide complete solutions for your laboratory.  Combining innovative engineering with high standards of quality, Agilent designs and manufactures high-performance equipment for processes that are revolutionizing pharmaceutical, biotech, and genomic research.


If you have not logged into the website then please enter your details below.



 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month

Top 10 most popular articles this month

 

Today's Picks

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


 

Media Partners