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Dionex Releases Standalone Ultrahigh-Pressure Pump for Proteomics Workflows Using Liquid Chromatography

Dionex Corporation is pleased to announce the expansion of its family of industry-leading Rapid Separation LC (RSLC) systems with the new UltiMate® 3000 NCP-3200RS nano/capillary LC pump module. This new standalone ultrahigh-pressure pump features an extensive flow-pressure footprint, delivering continuous, direct flow rates from 20 nL/min to 50 μL/min at backpressures of up to 800 bar (11,600 psi) for nano, capillary, and micro LC analyses.

The pump can be used to configure an entry-level RSLCnano system for reversed-phase separation of peptides in LC-MS proteomics workflows. Alternatively, the pump can be used as an add-on module to our industry-leading RSLCnano system for advanced applications, such as automated off-line 2D-LC or tandem nano LC. Tandem nano LC can be an attractive technique for customers who want to improve their mass spectrometry use and increase sample throughput. Dedicated application kits have been introduced for the RSLCnano system, such as automated off-line RP × RP of peptides and tandem nano LC, to facilitate instrument installation and get effective data from the first experiments.

In addition, Dionex has also released new biocompatible switching valves, featuring a maximum pressure specification of 345 bar (5,000 psi), for sample injection and column switching. These values are intended for use with the RSLCnano system, to provide a biocompatible sample flow path that can be important to customers dealing with the analysis of phosphopeptides.

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