Latest Application Note from Wyatt Technology Demonstrates How Absolute Molar Mass can be Measured Using UHPLC SEC-MALS

Thermo Fisher Scientific has developed a rapid and reliable sample preparation procedure and ion chromatography-based method to determine the phytate hydrolysis products in dried distillers grains with solubles (DDGS).

Application Note 1070: Determination of Inositol Phosphates in Dried Distillers Grains with Solubles demonstrates the determination of 25 inositol phosphate isomers—InsP2–InsP6—in an in-house prepared reference solution. Separation is achieved in less than 42 minutes using a column designed for high-resolution separations of carbohydrates. Using this separation, inositol phosphates are determined in DDGS....

Thermo Fisher Scientific has developed an ion chromatography (IC)-based method for the determination of ammonia in adenosine that can easily meet the sensitivity requirements in the U.S. Pharmacopeia (USP) adenosine monograph.

Application Note 1072: Ion Chromatography Assay for Ammonia in Adenosinedemonstrates that this proposed IC method for the determination of ammonia in adenosine enables an analysis time of 5 min, thereby yielding high sample throughput. This IC assay avoids the hazardous chemicals used in the color-based ammonia assay in the USP adenosine monograph...

Thermo Fisher Scientific has developed a reproducible and accurate ion chromatography (IC) method for determining monochloroacetic acid (MCAA) in carbocisteine drug substance and two monochloroacetic acid drug products, thus enabling efficient quality control of the carbocmanufacturing processisteine.

Application Note 1076: Determination of Monochloroacetic Acid in Carbocisteine demonstrates that this IC method is easily executed using a reagent-free IC system controlled by data management software. The analyst simply adds water and samples to separate and accurately determine MCAA using a hydroxide-selective anion-exchange column (Thermo Scientific^™ Dionex^™ IonPac^™ AS11-HC column)...

Bruker has released an application note detailing a simple and convenient method for the quantification of trace pharmaceutical and personal care products (PPCPs) in clean water, using Bruker’s EVOQ triple quadrupole liquid chromatography mass spectrometer (LC-MS/MS).

Children are exposed to intakes of heavy metals from a wide variety of sources. Chromium (VI) in particular represents a potential hazard, as it is absorbed from food and drinking water, from the air that is breathed, from textiles, from utensils that contain metal and from toys.

The analytical determination of chromium (VI) content in toys for compliance with limit values is described in the European standard DIN EN-71-3-2013 (Safety of toys Part 3 – Migration of certain elements). The standard differentiates between three categories, depending on the kind of toys...

Thermo Fisher Scientific has developed a new gradient high-performance liquid chromatography methods that use a spectro-electro array platform

Thermo Fisher Scientific has developed a new gradient high-performance liquid chromatography methods that use a spectro-electro array platform to measure specific analytes in beer samples, distinguish between different samples, and study beer stability. Application Note 1065: Gradient HPLC Method for Analysis of Beer Polyphenols, Proanthocyanidins, and Bitter Acids Using a Novel Spectro-Electro Array Platform demonstrates a method that provides accurate and sensitive measurements that cannot be obtained using ultraviolet detection alone, as well as metabolomic approaches that can be used to study fermentation, product stability, and authenticity issues...

Wyatt Technology has published a new whitepaper

Wyatt Technology Corporation, the world leader in absolute macromolecular characterization instrumentation and software, has published a new whitepaper on characterizing molecular structure in synthetic and natural polymers by multi-angle light scattering. Authored by Stepan Podzimek, Scientific Consultant for Wyatt Technology, the paper details the basic principles of the detection and characterization of branching by means of a multi-angle light scattering (MALS) detector and the various methods used...

Thermo Fisher Scientific has developed a rapid and robust high-performance anion-exchange chromatography

With pulsed amperometric detection method for the accurate determination of common sugars in acid-hydrolyzed biomass samples with high carbohydrate concentrations. Application Update 192: Carbohydrate Determination of Biofuel Samples demonstrates that this approach has a linear range suitable for handling high-concentration biomass samples with minimal sample treatment. This new method is fast, provides good sensitivity, has consistent response, and can be routinely used to determine carbohydrates in the feedstock for biofuel production...

Bruker releases application note for analysis of sulfonamides in honey using Bruker’s Advance EVOQ Elite LC-MS/MS system

Bruker Chemical and Applied Markets (CAM), Fremont, California, US: Bruker releases application note for analysis of sulfonamides in honey using Advance EVOQ Eliteruker has released an application note detailing a simple, fast and robust method developed for the quantitation of sulfonamides in honey, using Bruker’s EVOQ Elite triple quadruple liquid chromatography mass spectrometer (LC-MS/MS). Sulfonamides are a group of broad spectrum antibacterial drugs, used to prevent and treat bacterial growth in honey products...

Thermo Fisher Scientific has developed a reproducible and accurate high-performance liquid chromatography (HPLC) method combined with on-line solid-phase extraction for the sensitive determination of carbendazim in orange juice.

Application Note 1067: Determination of Carbendazim in Orange Juice demonstrates that this application—performed using a rapid separation liquid chromatography system—simplifies sample preparation and improves HPLC detection sensitivity with a method detection limit of carbendazim in orange juice of 0.2 μg/L. Although carbendazim is a fungicide used in many countries to improve crop production....

Thermo Fisher Scientific is pleased to announce a new technical note TN 139

Thermo Fisher Scientific is pleased to announce a new technical note TN 139: Determination of Anions in Fracking Flowback Water From the Marcellus Shale Using Automated Dilution and Ion Chromatography which demonstrates the use of in-line conductivity measurement to trigger automated dilution prior to separation and quantification of inorganic ions and organic acids in Marcellus Shale fracking flowback samples using ion chromatography...

New Insights Published on Key Protein Interactions Involved in the Bacterial Flagellar Motor Complex Show Promise for Other Difficult Biomolecular Systems

New Application Note from Wyatt Technology Showcases the Ability of CG-MALS to Quantify Complex Proteins. Wyatt Technology Corporation, the world leader in absolute macromolecular characterization instrumentation and software, has today published a new application note highlighting a study on the complex interactions between proteins and how they modulate the rotational direction of bacteria flagella....

Thermo Fisher Scientific is pleased to announce a new technical note which demonstrates high resolution chromatographic separations of organic acids in beer samples.

Organic acids are end products of yeast fermentation critical to the flavor of beer, but are also products of bacterial fermentation that introduce a sour flavor, either purposely or unintentionally due to spoilage. Technical Note 126: Determination of Organic Acids in Beer Samples Using a High-Pressure Ion Chromatography System specifically discusses the separation of organic acids in beer samples using a Thermo Scientific™ Dionex™ IonPac™ AS11-HC-4μm anion-exchange column....

Malvern Instruments has posted a new application note demonstrating the use of the company’s Viscotek SEC-MALS 20 multi angle light scattering (MALS) detector for size exclusion chromatography (SEC) in the analysis of monoclonal antibodies.

The application note, ‘Molecular weight measurements of an antibody using Viscotek SEC-MALS 20’, reports experimental data for the analysis of aggregates within monoclonal antibody formulations and can be freely downloaded. Monoclonal antibodies are being targeted for use in a growing range of therapeutic applications. Measuring the molecular weight of the protein, monomer, oligomers and aggregates within the sample and in formulations is an important part of assessing their safety and efficacy as drugs.....

Bruker has released an application note on the evaluation of matrix effects on the analysis of carbendazim in orange juice and wine using the EVOQ Elite liquid chromatography mass spectrometer (LC-MS/MS).

Carbendazim is a fungicide and a known endocrine with potentially harmful effects on human health. The US Food and Drug Administration (FDA) has limited the concentration of carbendazim in imports to 10 ppb. Using the EVOQ, Bruker has developed a quantitative Multiple Reaction Monitoring (MRM) method for carbendazim analysis in orange juice and wine matrices. The EVOQ’s sensitivity was demonstrated as detections of 0.005 ppb were made, far below the FDA limit. The application note is available to download...

Genevac has developed a protocol using its SuperCool 75 condenser

Genevac has developed a protocol using its SuperCool 75 condenser that, in conjunction with the Genevac HT-12 High Throughput Evaporator, has been proven to set a new benchmark for enhanced evaporation and lyophilisation results. The SuperCool 75 chills to -75C and operates at higher power than any other commercially available condenser, opening the door to greatly improved results with the Genevac LyoSpeed fast lyophilisation process for preparing powdered samples from HPLC fractions...

Thermo Fisher Scientific has developed a reliable method for determining levels of dithiocarbamate fungicides (DTCs) with high precision using gas chromatography-mass spectrometry (GC-MS).

The complex extraction and identification of DTCs is performed on various food samples after a thorough method validation. Application Note 10333, Analysis of Dithocarbamate Pesticides by GC-MS, compares DTC levels in various food samples to matrix-matched calibration standards. SIM mode analysis provides high selectivity with easy-to-integrate chromatograms. The analysis uses a Thermo Scientific™ TRACE GC Ultra™ gas chromatograph equipped with a Thermo Scientific™ TriPlus™ RSH liquid autosampler coupled to a Thermo Scientific™ ITQ™ 900 ion trap mass spectrometer...

The launch of Malvern Instruments’ Viscotek SEC-MALS 20 chromatography detector has triggered record downloads of one of the supporting white papers ‘Static light scattering technologies for GPC/SEC explained’, underlining the enthusiasm amongst users for straightforward information on GPC/SEC analysis.

The recent addition of Viscotek SEC-MALS 20 to Malvern’s range of detectors puts the company in the unique position of being the sole provider of every commercially available light scattering technology for GPC/SEC. Customers can therefore be assured of a truly unbiased choice of detector solutions. The popular white paper is just one of a suite of educational information and application notes provided by Malvern’s specialist team to help users select the right detector for their application...

Thermo Fisher Scientific has developed a simple and economical size-exclusion chromatography (SEC) analysis method that enables nuclear power plants (NPPs) with pressurized water reactors to determine low concentrations of polyacrylic acid (PAA) in secondary feed water containing ethanolamine (ETA) and hydrazine.

Application Note 1058: Determination of Polyacrylic Acid in Nuclear Power Plant Pressurized Water Reactor Secondary Feed Water demonstrates that a SEC column separation with detection at 200 nm can determine PAA with a method detection limit of 2.6 μg/L. This method uses water as the eluent to maximize sensitivity and eliminate the need for eluent preparation...

Thermo Fisher Scientific has developed an on-line solid phase extraction (SPE)high-performance liquid chromatography (HPLC) with ultraviolet absorbance detection method to determine the 14 explosives and related compounds specified in U.S.

Environmental Protection Agency Method 8330A in environmental water samples. Application Note 1066: Sensitive Determination of Explosive Compounds in Waterdemonstrates that this approach eliminates the labor associated with off-line extraction and provides a convenient method that achieves reduced method detection limits (less than 0.35 μg/mL) when determining these compounds in water...

Thermo Fisher Scientific has developed a reliable method for determining phthalates in alcoholic beverages by single-quadrupole gas chromatography-mass spectrometry. The method met the special testing requirements set by the China method GB/T 21911-2008 for determining phthalates in food.

Application Note 10339, Determination of Phthalates in Liquor Beverages by Single Quadrupole GC-MS, describes a sensitive and accurate single-quadrupole GC-MS method for determining levels of phthalate plasticizers in alcoholic beverages. The method is sensitive, rapid, and accurate, and covers a wide linear range to meet the need for trace level detection of phthalate esters in different types of beverages. The analysis uses a Thermo Scientific™ ISQ™ Series single-quadrupole GC-MS coupled to a Thermo Scientific™ TRACE™ 1310 GC.