Thermo Fisher Scientific has developed efficient high-performance liquid chromatography (HPLC) methods combined with ultraviolet detection to determine carbonyl compounds found in vehicle passenger cabin air samples.
Application Note 1056: Determination of Carbonyl Compounds in Vehicle Cabin Compartments demonstrates that all 13 carbonyl compounds specified in California Air Resources Board Method No. 1004, International Organization for Standardization 16000-3:2011, U.S. Environmental Protection Agency (EPA) Compendium Method TO-11A, and the Chinese HJ/T 400-2007 are derivatized with 2,4-dinitrophenylhydrazine and well separated on a reversed-phase column in less than 10 minutes...
Thermo Fisher Scientific has developed an accurate and reproducible method that uses an innovative ion chromatography system for the determination of methylamine (or monomethylamine, MMA) in drug products.
Application Note (AN) 1057: Determination of Methylamine in Drug Productsdemonstrates simple off-line sample preparation followed by an on-line step to complete the sample preparation before analysis. This approach requires only 32 min per analysis. The method uses a cation-exchange column and the eluent is produced by aneluent generator to preclude the labor and potential error associated with eluent preparation...
Thermo Fisher Scientific has developed a method for the fast determination of PCDD/Fs in sediments, soils, bottom and fly ash (as incineration by-products) using gas chromatography-tandem mass spectrometry (GC-MS/MS).
Application Note 10336,Determination of PCDD/Fs in Environmental Samples using Accelerated Solvent Extraction and GC-MS/MS,describes a sensitive and accurate GC-MS/MS method for determining levels of dioxins and furans in various environmental samples using GC-MS/MS. Instrumental LOQs using GC-MS/MS were calculated in the low fgL concentration ranges...
Thermo Fisher Scientific has developed an ion chromatography(IC)-based method for the accurate determination of ammonia in tobacco smoke.
Ammonia is on the U.S. Food and Drug Administration’s (FDA) list of toxic compounds in tobacco smoke. Application Note 1054: Determination of Ammonia in Tobacco Smoke describes an approach that achieves better resolution of ammonium from other analytes present in tobacco smoke when compared to other IC methods and therefore is not subject to interference from small amines. The method has high precisions, a low detection limit (0.01 mg/L), and good recoveries (86–115%)...
A new article detailing a round table discussion on quality by design (QbD) authored by leading industry experts including Spinnovation Biologics CEO Frederic Girard is now available online.
Entitled ‘QbD and PAT in Upstream and Downstream Processing’, the article details the fundamental principles of QbD, how it is used to improve process control, and the impact technological advances have made on QbD biopharmaceutical upstream process optimization. Originally published in the July addition of Biopharm International the full article is now available to read...
Thermo Fisher Scientific is pleased to announce a new technical note on fast separations in a carbonated beverage using increased flow rates on our high-pressure capillary Ion Chromatography (IC) system, the Thermo Scientific Dionex ICS-5000+ Reagent Free HPIC system.
Technical Note 118: Fast Separations of Anions and Organic Acids in a Carbonated Beverage Using High-Pressure Capillary IC describes the determinations of organic acids in these carbonated beverages. The beverage industry uses these acidulants and flavoring agents to maintain beverage freshness, to minimize microbiological growth and to add a characteristic flavor...
Thermo Fisher Scientific has developed a high-performance liquid chromatography (HPLC) method for a fast and sensitive determination of paraquat and diquat in tap and environmental water samples.
This approach uses on-line solid phase extraction (SPE) and achieves method detection limits that meet the requirements of both U.S. Environmental Protection Agency Method 549.2 and European Union 98/83/EC. Application Note (AN) 1051: Sensitive and Rapid Determination of Paraquat and Diquat in Tap and Environmental Water demonstrates use of a mixed-mode column to provide sufficient retention, excellent resolution, and good peak shape for these herbicides...
Thermo Fisher Scientific is pleased to announce a new technical note discussing rapid separation of inorganic cations in municipal wastewater using our high pressure capillary Ion Chromatography system, the Thermo Scientific Dionex 5000+ ICS HPIC system.
Technical Note 121: Fast Determinations of Inorganic Cations in Municipal Wastewater Using High-Pressure Capillary IC demonstrates how the separation of inorganic cations are important to municipal drinking water and wastewater treatment plants for compliance monitoring. Cationic determinations are necessary as part of the water monitoring program’s secondary water specification of acceptable taste....
Thermo Fisher Scientific is pleased to announce a new technical note on the fast separations of inorganic ions in saline solution samples.
TN 130: Fast Determinations in Salton Sea Samples Using a High-Pressure IC System demonstrates fast separations of inorganic anions and cations in a Salton Sea surface water sample and a neighboring municipal drinking water sample using ion-exchange chromatography. Monitoring salinity levels in estuarine ecosystems is critical to preventing salinity-specific diseases...
Thermo Fisher Scientific has developed an accurate and reproducible high-performance liquid chromatography (HPLC) method for the single-injection determination of tolterodine and tartrate—the active pharmaceutical ingredient (API) and its counterion—in an encapsulated drug product containing tolterodine tartrate.
Application Note 1047: Simultaneous Determination of Tartaric Acid and Tolterodine in Tolterodine Tartrate demonstrates that this method requires only 13 minutes per analysis. This new approach uses a trimode silica-based column and a detector that is ideal for the determination of counterions, many of which lack a chromophore. Pharmaceutical companies must ensure that a drug product contains the appropriate amount of its API. This is typically validated with an HPLC-based assay...
Malvern Instruments has published ‘Static Light Scattering Technologies For GPC/SEC Explained’, a valuable guide for anyone assessing the value of a light scattering detector for gel permeation/size exclusion chromatography (GPC/SEC).
Covering the theory and practice of molecular weight measurements using static light scattering, this basic guide serves as both an introduction for those with no prior knowledge of the technique and a reference document for anyone looking to enhance their understanding. The white paper is available as a free download from the Malvern Instruments website
Read MoreExtraction of Metaldehyde, Geosmin and 2-Methylisoborneol from WaterJul 16, 2013
Kinesis has published a new Application Note: Extraction of Metaldehyde, Geosmin and 2-Methylisoborneol from Water
The latest TELOS® SPE Application Note from Kinesis highlights the extraction of Metaldehyde, Geosmin and 2-Methylisoborneol from Water. Metaldehyde is a molluscicide whose use has markedly increased in recent years as a result of changing agricultural practices. Geosmin and 2-methylisoborneol (MIB) are naturally occurring alicyclic alcohols which possess potent earthy/musty taste and odour tainting properties. They can occur in raw and potable waters as metabolites derived from various micro-organisms...
Thermo Fisher Scientific has developed an ion chromatography method for nuclear power plants (NPPs) that determines trace concentrations (less than 1 µg/L) of fluoride, chloride, and sulfate in simulated secondary feed water that contains ethanolamine, hydrazine, and polyacrylic acid in just 20 minutes.
Application Brief (AB) 151: Determination of Trace Anions in Nuclear Power Plant Secondary Feed Water Containing Polyacrylic Acid demonstrates excellent retention time and peak area reproducibilities from 69 consecutive injections of a spiked simulated sample...
Thermo Fisher Scientific has developed an efficient high-performance liquid chromatography (HPLC) method using ultraviolet detection for the simultaneous determination in drinking water of all 19 phthalate compounds listed in key environmental regulatory documents.
Application Note 1045: Determination of Phthalates in Drinking Water by UHPLC with UV Detection demonstrates that the phthalates listed in European Union Directive 2005/84/EC, U.S. Environmental Protection Agency Methods 606 and 8061A, the Chinese HJ/T 72-2001, and the Standardization Administration of China GB/T 20388-2006 and GB/T 21911-2008 are well separated on a reversed-phase HPLC column in less than 25 minutes...
In a new paper published in Polymers & Polymer Composites (Vol 21, No 1, 2013), researchers at Kingston University in the UK report the results of continuing work to examine the effects of gamma irradiation on intraocular lenses (IOLs) used in cataract surgery. Triple detection gel permeation chromatography (GPC) using the Viscotek TDAmax system from Malvern Instruments was a key analytical tool in this work.
Baljit Ghatora and co-authors examined medical grade poly(methyl methacrylate) (PMMA), which is used especially widely in developing countries to make rigid intraocular lenses and for which low cost, convenient gamma irradiation is increasingly the preferred method for sterilisation. Previous reports have suggested that gamma irradiation has the potential to alter the structural features of the polymer, such as molecular weight and molecular size distribution, which in turn may impact the efficacy and performance of IOLs....
Read MoreJ G Finneran Associates introduces the 96-Well Multi-Tier Microtiter Plate System (Patented)Jun 25, 2013
An improvement on existing 96-Well systems, the Multi-Tier Plate is a modular, reusable system featuring a base plate capable of securing 0.5, 1.0, 1.5, or 2.0 mL conical glass, flat bottom and polypropylene vials.
The vented design of the base plate assures uniform heating and cooling when immersed in a water bath. The cover locks the liner in place and prevents vial pullout. The prescored PTFE/Silicone/PTFE liner permits easy needle penetration, while the Molded PTFE/Silicone liner locks into each of the 96 vials and reseals after multiple injections thus reducing the chance of solvent evaporation and cross contamination....
A new application note from Porvair Sciences details a protocol for its Chromatrap® ChIP assay kit that has been developed to enrich epigenetic marks from primary human tissue cells.
Chromatrap® Pro-A, the novel solid-based matrix for chromatin immunoprecipitation assays (ChIP) has been successfully used for isolation of high quality chromatin from difficult biopsy material. Focusing on an application to precipitate epigenetic marks from primary endometrial stromal cells - results show high abundant epigenetic marks from 1 million cells isolated from a tissue biopsy, with selective amplification of positive gene targets relative to negative IgG and negative gene loci....
Genevac, world leaders in solvent removal technology, has over 20 year’s experience of supplying evaporators for removal of solvents from oil, fat and fuel samples.
Oils, fats and fuels are normally isolated from natural sources using extraction or separation techniques that present the final sample in a solution of organic solvent. Before analysis such samples must be dried or concentrated. Whether extracting and recovering additives, evaluating lipid content of foods, characterising reclaimed cooking oils before reprocessing / conversion to biofuels, or investigating drillling test samples - oils, fuels and fats can often prove difficult to dry without using very high temperatures which may lead to loss of the more volatile fractions within the sample....
Read MoreDebunking Myths about Peak Area Response of Glycoprotein OligosaccharidesMay 20, 2013
Thermo Fisher Scientific has released a technical note (TN) that challenges the common misconception that there is significant variation in high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) peak area response of oligosaccharides.
TN 133: HPAE-PAD Peak Area Response of Glycoprotein Oligosaccharides summarizes the peer-reviewed scientific literature concerning this subject. This review concludes that the variation in HPAE-PAD oligosaccharide response with structure is minimal, especially among sets of similar oligosaccharides such as those found on many glycoproteins.....
RSA (Reduced Surface Activity) Glass vials are produced by a revolutionary new patent pending production process that virtually eliminates all silanols and produces glass which has a greatly reduced surface activity for basic compounds
RSA Glass vials are not coated or silanized. The lack of surface silanols make RSA Glass vials an excellent choice for many applications. If you are working with sensitive compounds commonly found in natural products, biological samples or chemical synthesis, the glass wall of your vial could be a problem for basic compounds. With RSA Glass vials this problem is eliminated....
Read MoreStandard-Compliant Method Determines Phenylurea Compounds in Tap WaterMar 19, 2013
Thermo Fisher Scientific has developed an efficient high-performance liquid chromatography (HPLC) method for the sensitive determination of nine phenylurea compounds in drinking water and commercial bottled beverages with method detection limits that meet those reported in U.S. Environmental Protection Agency Method 532 and concentration limits set in the European Commission’s Council Directive 98/83/EC
Application Note (AN) 1046: Determination of Phenylurea Compounds in Tap Water and Bottled Green Tea demonstrates that use of on-line solid-phase extraction(SPE) and ultraviolet detection eliminates the need for expensive and time-consuming off-line SPE. Phenylurea compounds are widely used as agricultural pesticides. Due to their slow degradation, they are frequently detected in surface waters at concentrations above 0.1 μg/L, which is higher than the European Commission’s drinking water limit often used as a quality standard for natural water...
A technical article from Genevac is available that details how its HT-24 centrifugal evaporation system is assisting a commercial flavours and fragrance company in its quest to find and isolate novel flavour ingredients from nature
Natural product derived discovery of novel flavour molecules is a highly complex process. Natural product sources yield complex extracts containing many components, which often do not lend themselves to processing and analysis. Conventionally, many steps of fractionation and evaluation are necessary until a compound is obtained from natural sources at the purity required for structural analysis. In the article a state of the art fractionation process using a Sepbox® Preparative HPLC - SPE system* and a Genevac® HT-24 centrifugal evaporator is described.
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Ammonia is on the U.S. Food and Drug Administration’s (FDA) list of toxic compounds in tobacco smoke. Application Note 1054: Determination of Ammonia in Tobacco Smoke describes an approach that achieves better resolution of ammonium from other analytes present in tobacco smoke when compared to other IC methods and therefore is not subject to interference from small amines. The method has high precisions, a low detection limit (0.01 mg/L), and good recoveries (86–115%)...
Technical Note 121: Fast Determinations of Inorganic Cations in Municipal Wastewater Using High-Pressure Capillary IC demonstrates how the separation of inorganic cations are important to municipal drinking water and wastewater treatment plants for compliance monitoring. Cationic determinations are necessary as part of the water monitoring program’s secondary water specification of acceptable taste....
Covering the theory and practice of molecular weight measurements using static light scattering, this basic guide serves as both an introduction for those with no prior knowledge of the technique and a reference document for anyone looking to enhance their understanding. The white paper is available as a free download from the Malvern Instruments website
Baljit Ghatora and co-authors examined medical grade poly(methyl methacrylate) (PMMA), which is used especially widely in developing countries to make rigid intraocular lenses and for which low cost, convenient gamma irradiation is increasingly the preferred method for sterilisation. Previous reports have suggested that gamma irradiation has the potential to alter the structural features of the polymer, such as molecular weight and molecular size distribution, which in turn may impact the efficacy and performance of IOLs....
The vented design of the base plate assures uniform heating and cooling when immersed in a water bath. The cover locks the liner in place and prevents vial pullout. The prescored PTFE/Silicone/PTFE liner permits easy needle penetration, while the Molded PTFE/Silicone liner locks into each of the 96 vials and reseals after multiple injections thus reducing the chance of solvent evaporation and cross contamination....
Oils, fats and fuels are normally isolated from natural sources using extraction or separation techniques that present the final sample in a solution of organic solvent. Before analysis such samples must be dried or concentrated. Whether extracting and recovering additives, evaluating lipid content of foods, characterising reclaimed cooking oils before reprocessing / conversion to biofuels, or investigating drillling test samples - oils, fuels and fats can often prove difficult to dry without using very high temperatures which may lead to loss of the more volatile fractions within the sample....
TN 133: HPAE-PAD Peak Area Response of Glycoprotein Oligosaccharides summarizes the peer-reviewed scientific literature concerning this subject. This review concludes that the variation in HPAE-PAD oligosaccharide response with structure is minimal, especially among sets of similar oligosaccharides such as those found on many glycoproteins.....
RSA Glass vials are not coated or silanized. The lack of surface silanols make RSA Glass vials an excellent choice for many applications. If you are working with sensitive compounds commonly found in natural products, biological samples or chemical synthesis, the glass wall of your vial could be a problem for basic compounds. With RSA Glass vials this problem is eliminated....
Application Note (AN) 1046: Determination of Phenylurea Compounds in Tap Water and Bottled Green Tea demonstrates that use of on-line solid-phase extraction(SPE) and ultraviolet detection eliminates the need for expensive and time-consuming off-line SPE. Phenylurea compounds are widely used as agricultural pesticides. Due to their slow degradation, they are frequently detected in surface waters at concentrations above 0.1 μg/L, which is higher than the European Commission’s drinking water limit often used as a quality standard for natural water...
Natural product derived discovery of novel flavour molecules is a highly complex process. Natural product sources yield complex extracts containing many components, which often do not lend themselves to processing and analysis. Conventionally, many steps of fractionation and evaluation are necessary until a compound is obtained from natural sources at the purity required for structural analysis. In the article a state of the art fractionation process using a Sepbox® Preparative HPLC - SPE system* and a Genevac® HT-24 centrifugal evaporator is described.