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Separate Targeted Carbonyl Compounds in Less Than 10 Minutes

UHPLC Focused

Thermo Fisher Scientific has developed efficient high-performance liquid chromatography (HPLC) methods combined with ultraviolet detection to determine carbonyl compounds found in vehicle passenger cabin air samples.

Application Note 1056: Determination of Carbonyl Compounds in Vehicle Cabin Compartments demonstrates that all 13 carbonyl compounds specified in California Air Resources Board Method No. 1004, International Organization for Standardization 16000-3:2011, U.S. Environmental Protection Agency (EPA) Compendium Method TO-11A, and the Chinese HJ/T 400-2007 are derivatized with 2,4-dinitrophenylhydrazine and well separated on a reversed-phase column in less than 10 minutes.

Many carbonyl compounds are known to cause adverse effects on human health and thus have been listed by the U.S. EPA in the Health Effects Notebook for Hazardous Air Pollutants. People are subject to toxic exposure during the manufacture of these carbonyl compounds, which are widely used in large quantities in automotive manufacturing. Carbonyl compounds can also be hazardous to driver and passenger health through the release of these compounds into vehicle cabin compartments. People in modern society now spend more time in vehicles due to lengthy commutes, long-distance travel, and frequent traffic jams. There are limits for the amounts of the carbonyl compounds that can be in the cabin compartment of a new automobile. Therefore, it is important to establish effective methods for the determination of these compounds found in the air inside vehicle cabins.


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About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of $12 billion, we have approximately 39,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through three premier brands, Thermo Scientific, Fisher Scientific and Unity™ Lab Services, which offer a unique combination of innovative technologies, convenient purchasing options and a single solution for laboratory operations management. Our products and services help our customers solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity.


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