Members Login
Channels
Special Offers & Promotions
HPLC Assay of Gentamicin Sulfate Congeners in Just 15 Minutes
Thermo Fisher Scientific is pleased to announce a direct-detection reversed-phase high-performance liquid chromatography (HPLC) chromatographic assay method that separates the congeners in gentamicin sulfate and gentamicin sulfate-containing ointments, solutions, and creams in just 15 minutes
Application Note 1005: Gentamicin Sulfate Assay by HPLC with Charged Aerosol Detection demonstrates that this approach is faster than previously published methods, with excellent separation between the gentamicin congeners and resolution of many impurities that can be present in fermentation-manufactured products.This method uses a silica-based polar-embedded column that provides enhanced hydrolytic stability from pH 1.5-10 and allows resolution of a wide variety of polar and nonpolar analytes. Use of this column allows a reduced concentration of trifluoroacetic acid, thus improving method ruggedness. Use of charged aerosol detection eliminates the need for derivatization. With three sample matrixes analyzed, the quantification of gentamicin base had good recoveries and was consistent with the label claim of these products, both indicators of an accurate assay.
This application note and many others can be found at www.thermoscientific.com/dionex
under the Documents tab.
News Channels
- Latest News
- New Laboratory Products
- Industry News
- Laboratory Automation | IT Solutions
- Microscopy | Image Analysis
- Separation Science
- Spectroscopy
- Research | Case Studies
- Video Presentations
- Events | Conferences
Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!
Popular this Month
Top 10 most popular articles this month
Today's Picks
Looking for a Supplier?
Search by company or by product
Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.
Lab Bulletin is published by newleaf marketing communications ltd.
Media Partners