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Determination of Steviol Glycoside in Sweeteners

Determination of Steviol Glycoside in SweetenersThermo Fisher Scientific Inc. is pleased to announce an economical new method that uses high-performance liquid chromatography (HPLC) with charged aerosol and UV detections to determine steviol glycosides in consumer sweeteners. Application Note 293: Steviol Glycoside Determination by HPLC with Charged Aerosol and UV Detections Using the Acclaim Trinity P1 Column demonstrates that only 3-9 mL of mobile phase/sample are required, compared to 25-60 mL/sample for the Joint FAO/WHO Commission on Food Additives ( JECFA) and U.S. Pharmacopeia (USP) methods, respectively. The reduction in mobile phase use saves both time and resources during mobile phase preparation and also reduces waste.

Using the Thermo Scientific Acclaim Trinity P1 column, the proposed method resolves the steviol glycosides of interest. Quantification of the two principal glycosides, stevioside and rebaudioside A, can be accomplished by both detection methods. Charged aerosol detection has the advantage of distinguishing additional components in the sample that are not UV absorbing, allowing for a flexible method to check sweetener purity.

This application note and many others can be found at under the Documents tab.

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of nearly $11 billion, we have approximately 37,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through two premier brands, Thermo Scientific and Fisher Scientific, which offer a unique combination of continuous technology development and the most convenient purchasing options. Our products and services help accelerate the pace of scientific discovery, and solve analytical challenges ranging from complex research to routine testing to field applications.

All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

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