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Rapid Analysis of Tobacco Smoke Biomarkers

publication date: Aug 18, 2022
author/source: YMC Europe GmbH


New Application note from YMC - Fast UHPLC Analysis of Biomarkers Related to Tobacco Smoke Exposure using YMC-Triart C18 and YMC-Triart Diol-HILIC

Both RP and HILIC allow for high resolution peaks and fast separation. In addition to nicotine, the metabolite cotinine is also determined. The challenge here is that this is only detectable for a short time. NNAL, a breakdown product of the lung carcinogen NNK, is also suitable. In addition, human cells are exposed to oxidative stress when smoking. Therefore, typically the characteristic biomarkers 8-OhdG and m7Gua are also evaluated.


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