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Due to a warming global environment there has been a rise in occurrences of cyanobacteria blooms in lakes which can generate cyanotoxins. With surface water a major source of drinking water, the presence of cyano bacterial blooms pose a potential threat to human health. Using Ultra-high Performance Liquid Chromatography (UPLC) researchers have been able to...
The cell membrane is a bilayer of phospholipids with embedded proteins. It contains cholesterol that determines the membrane’s fluidity, permeability and activity of membrane proteins. Changes in membrane cholesterol are implicated in diseases such as Alzheimer’s and cancer, demanding its investigation....
The authors describe how for every chromatin preparation it is essential to check the chromatin is sheared to fragments between 100-500 bp. They emphasise using a microfluidics platform is the most accurate measure of quantifying DNA. Problems associated with over or under sheared Chromatin fragments are discussed and a protocol for optimised enzymatic shearing described.....
A case study describing the experiences of Niguarda Hospital, Milan, in assessing the new DxN VERIS Molecular Diagnostics System is now available from Beckman Coulter. The case study describes the workflow improvements and reduced turnaround times that the hospital’s Molecular Biology Laboratory was able to achieve for viral load measurements using the DxN VERIS system. One of Italy’s leading General Hospitals...
The paper details how scientists at leading Asian pharmaceutical company, WuXi AppTec are using the ambr® 15 automated micro bioreactor system to mimic bench-top and production scale bioreactors, producing comparable results with cell growth, cell metabolism and protein production, including similar protein titre to BIOSTAT® STR single-use bioreactors.In the article, one case study describes how...
In undertaking these experiments, independent researchers found that the unique electronically adjustable tip facility on the VOYAGER not only generated time savings of up to 80% from minimising the number of required manual handling steps but also improved pipetting reproducibility and reduced pipetting errors.
The paper details how scientists at the Australian Water Quality Centre (AWQC) have for the first time independently validated that results from the ProtoCOL 3 automated colony counter are as accurate as manual colony counting. The article describes how microbiologists at AWCQ, a leading NATA (National Association of Testing Authorities) accredited water testing laboratory in Adelaide, Australia cultured microorganisms isolated from...
Preparation of reproducible serial dilution assays, especially in 384-well plates, is a challenging task because they require many pipetting actions and are highly dependent on an operator's technique and experience. However, observing good pipetting practice helps immensely to improve reproducibility and accuracy. In this white paper the author focuses on a selection of good pipetting techniques, which if maintained offer the best chance of...
eXalt™ is a proprietary Genevac technology designed to control evaporation using restricting baffles. Using this technology multiple solvents can be screened in parallel to identify optimum crystallisation conditions for amorphous materials. In the pharmaceutical industry, physical form can pose a significant challenge for small molecule drug discovery projects transitioning between the optimisation and characterisation phases. A crystalline form is desirable...
Sphere Medical, innovator in critical care monitoring and diagnostics equipment, has published a white paper focusing on the mitigation of common pre-analytical errors associated with arterial blood gas analysis [1]. The informative paper is available for download from Sphere’s online clinical resource centre and explores how and why pre-analytical errors occur, as well as the impact of an error on analyte levels in the blood sample. It also considers the...
Genome-wide mapping of protein-DNA interactions is essential for a complete understanding of gene regulation. A detailed map of epigenetic marks and transcription factor binding is necessary for deducing the regulatory networks that underpin gene expression in a variety of biological systems. The most widely used tool for examining these interactions is ChIP followed by massively parallel sequencing (ChIP-seq). The new application note reports on how the Chromatrap...
Driven by the rapid growth in proteomics and protein research applications the need for storage tubes where interaction between the sample and the tube surface is minimised has grown exponentially. The need for storage tubes that exhibit ultra-low binding properties is critical to these research fields due to the low concentrations of proteins typically found in samples. In the study – protein binding measurement tests were performed on Micronic...
In today’s networked economy, organizations are challenged with volatile market requirements, massive connectivity driven by the "Internet of Things", and increased product, process, and supply chain complexity. Enterprises are continually looking deeper into their operations and farther across their supply chains for opportunities to become more efficient. Labeling is frequently considered a background process that does not get...
Genome-wide mapping of protein-DNA interactions is essential for a complete understanding of gene regulation. A detailed map of epigenetic marks and transcription factor binding is necessary for deducing the regulatory networks that underpin gene expression in a variety of biological systems. The most widely used tool for examining these interactions is ChIP followed by massively parallel sequencing (ChIP-seq). The new application note reports on how...
Gilson’s MICROMAN E positive displacement pipette with its Capillary Pistons helps scientists counter common pipetting problems that may affect accuracy and precision when working with problem liquids. Leaks are known to occur when pipetting volatile solutions such as acetone, hexane and ethanol with an air-displacement pipette.
Taking the example of Malvern’s development of the Mastersizer 3000 particle size analyzer, the authors illustrate how instrument companies are aligning with the QbD philosophy. This offers pharmaceutical customers the reassurance of closely similar working practices and products that meet exacting customer standards. The central idea of QbD is that quality should be built into a product from the outset. It is an approach based on...
The four application notes cover 'Synthesis of Artemisinin using E-Seriesand UV-150 reactor' (Application Note 39), 'α-Photodecarboxylation of Phthaloyl Glycine' (38), 'Photochemical Transformation of Methyl Coumalate' (37) and '[2+2] Photocycloaddtion of Maleimide and 1-Hexyne' (36) and are available to download in full from the Vapourtec website. Vapourtec MD and founder Duncan Guthrie explained: "Taken together these...
Epigenetics is a fast-growing research area with wide applications, including disease mechanism profiling and personalized medicine strategies. ChIP is crucial to epigenetic research. Published in the latest issue of Nature Methods (11, 2014) the article provides an introduction to Chromatrap® 96 - a high-throughput ChIP analysis platform and demonstrates how it is able to profile up to 96 transcription factors and epigenetic modifications...
The analysis of liquid samples, including volatile liquids, can be done quite easily on the Exeter Analytical Model 440 system without sacrificing accuracy or precision and without the need for expensive liquid autosamplers. The new application note 233 describes how a special liquid sample preparation technique together with controlled introduction of the sealed capsules into the Model 440 eliminates the problems associated with...
