Search News Archives
Follow us on
Conferences | Events
Media Partners

New Application Note from BMG Labtech

publication date: Mar 16, 2017
 | 
author/source: BMG LABTECH

The fluorescent probe NR12S detects changes in plasma membrane cholesterol levels

BMG App NoteThe cell membrane is a bilayer of phospholipids with embedded proteins. It contains cholesterol that determines the membrane’s fluidity, permeability and activity of membrane proteins. Changes in membrane cholesterol are implicated in diseases such as Alzheimer’s and cancer, demanding its investigation.

NR12S is a fluorophore that exhibits emission maxima at 560 and 630 nm dependent on the liquid order of the membrane. Incorporation of the dye in a liquid ordered phase (increased cholesterol) shifts the emission to 560 nm, whereas in a liquid disordered phase (lower cholesterol) it emits at 630 nm. Hence, the 560/630 ratio correlates with the membrane cholesterol content.

Inhibition of cholesterol synthesis by lovastatin or incubation with methyl-ß-cyclodextrin decreased membrane cholesterol in hematological cells and was reported by decreases in the 560/630 ratio. Both emissions were conveniently measured on the filter-based FLUOstar® Omega microplate reader and could be analyzed with one-click using the MARS analysis software.


View the full application note


 



 

Popular this month


 

Lose the Solvent Headache with Cole-Parmer VapLock™ Closed Systems


Tomocube adds 3D Fluorescence Imaging to Holotomography Microscopy for World First


ZEISS Elyra 7 with Lattice SIM IntroducedZEISS Elyra 7 with Lattice SIM Introduced


Restek Now Has Even More of What You Need to Keep Your LC Flowing


Metrohm contributes to global monograph modernization initiative


Fused-Core Selectivity Toolkits for Superficially-Porous Particle Method Development


Introducing Renishaw’s New RA816 Biological Analyser


ID Kit – fast, easy, and accurate detection of heroin and other illicit drugs


Safe Evaporative Sample Preparation of High Potency APIs


Simplifying high content screening with the VIAFLO 96

 

 

Can't find what you are looking for?


Search by company or by product


Company Name:

Product:





Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd
Media Partners