Search News Archives
Follow us on
Conferences | Events
Lab Bulletin Media Partners

High Throughput ChIP-seq Assay for Genome-wide Protein-DNA Analysis

publication date: May 22, 2015
author/source: Porvair Sciences Limited

Chromatrap®, a business unit of Porvair Sciences, has published a new application note that describes how Chromatrap® ChIP-seq assays now enable unbiased, genome-wide understanding of protein-DNA regulatory networks. 

porvair chemistGenome-wide mapping of protein-DNA interactions is essential for a complete understanding of gene regulation. A detailed map of epigenetic marks and transcription factor binding is necessary for deducing the regulatory networks that underpin gene expression in a variety of biological systems. The most widely used tool for examining these interactions is ChIP followed by massively parallel sequencing (ChIP-seq). 

The new application note reports on how the Chromatrap® ChIP-seq kit when used with Illumina ChIP-seq library preparation kits and sequencing platforms is able to deliver high-quality DNA for sequencing. Due to Chromatrap®'s unique solid-phase matrix and the option of a 96 well plate for assays, sample throughput and reproducibility is increased owing to smaller sample volumes, centrifugal wash-steps and a matrix that does not require blocking. 

Compatible with direct, deep sequencing of enriched fragments, Chromatrap® ChIP-seq assays is shown to enable unbiased, genome-wide understanding of protein-DNA regulatory networks. Excitingly the inert solid-support matrix enables reproducible capture and genome-wide amplification of landmark regulatory complexes from low amounts of input chromatin. Further the results demonstrate how Chromatrap® ChIP-seq can be used to further understand the genome wide binding patterns of estrogen receptor in the human endometrium; demonstrating the utility of fast, multiplex analysis by massively parallel next-generation sequencing.

The Chromatrap® ChIP-seq kit provides a quicker, more efficient alternative to traditional bead-based ChIP assays. ChIP sample processing time is reduced to just 5 hours using up to 500,000 cells per IP. The spin-column technology allows for centrifugation at each wash step, improving efficiency and minimising handling errors often associated with magnetic beads. Throughput can also be increased with the availability of a 96-well plate format for users processing high-volume samples or antibody targets. Chromatrap® is a business unit of Porvair Sciences, a wholly owned subsidiary of Porvair plc.

more about chromatrap

more news from porvair




If you have not logged into the website then please enter your details below.


Popular this month


Win an INTEGRA VOYAGER adjustable tip spacing pipette

Thermo Fisher Scientific Announces Collaboration to Simplify Analysis of Complex Therapeutic Proteins

World’s first high throughput microplate reader for circular dichroism

Eppendorf Presents First Internally Developed CO2 Incubator

Thinking about buying a LIMS? Pick wisely!

Lab Innovations 2018 confirmed as a major hit with visitors, exhibitors and speakers

Take your microwell plate pipetting to the next level!

ZEISS and arivis AG Intensify Strategic Partnership

New RenataDX Screening System Delivers Fast, Accurate Results for Clinical Scientists

Stunning Weevil Eye Wins Forty-Fourth Annual Nikon Small World Competition



Can't find what you are looking for?

Search by company or by product

Company Name:


Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd