Follow us...

 

Search News Archives

Monthly Newsletter

 

Lab Bulletin May Newsletter

view the latest issue

Subscribe

News Channels

 

New Laboratory Products

 

Lab News

 

Microscopy & Image Analysis

 

Separation Science

 

Research & Case Studies

 

Literature

 

Videos

 

Events | Webinars

 

 

 

Conferences | Events

Chromatrap Announce Native ChIP Kit for Mass Spectrometry

publication date: Aug 17, 2017
 | 
author/source: Porvair Sciences Limited

ChIP Kit for Mass Spectrometry

Chromatrap announces that its popular Native ChIP kit is now fully compatible for mass spectrometry (MS) as a downstream process.

Combining Chromatrap®’s unique solid-state ChIP technology with MS enables researchers to obtain high quality data sets for chromatin proteomic profiling and mapping of histone modification patterns. 

The Chromatrap® Native ChIP (N-ChIP) kit is widely proven to ensure a high yield of DNA so that samples can be analysed by multiple downstream processes. Leveraging Chromatrap®’s unique solid-state technology, the N-ChIP kit eliminates the use of magnetic or agarose beads allowing users to quickly and easily recover ready-to-use, purified chromatin for downstream applications, including mass spectroscopy. Coupling MS to ChIP will allow researchers to shed light on the proteomic landscape of the extracted chromatin enabling them to begin to understand how proteins interact with repressed and active regions of the genome and their role in global gene regulation. 

Chromatin immunoprecipitation (ChIP) is a technique commonly used for mapping the DNA-protein interactions in cells, which are crucial for gene regulation. The technique enriches DNA fragments to which a specific protein of interest or histone modification is bound. Enriched sites can then be identified by qPCR, hybridisation to a microarray (ChIP-on-chip), or by sequencing. This powerful tool is now being further enhanced by coupling it directly to mass spectrometry, itself an invaluable and versatile tool for the characterisation of biomolecules.

In Native ChIP, the naturally occurring DNA-protein complexes are preserved in their “native” state by doing away with the need to cross-link and fix cells prior to extraction. Although there are a number of published protocols outlining methods for performing N-ChIP, Chromatrap uniquely offers researchers an efficient kit with an optimized protocol specific to this technique.

With the addition of MS to the Chromatrap repertoire of downstream applications, the company continues to expand the role of its ChIP kits across the epigenetic research sector. 

Chromatrap is a business unit of Porvair Sciences Ltd. with R&D and production laboratories based in the UK. Their extensive product range includes ChIP kits, DNA clean up kits and other innovative products for epigenetic research. Now cited in approaching thirty papers - Chromatrap® ChIP technology is rapidly becoming the method of choice for fast accurate ChIP assays.


more about chromatrap


more news from porvair


 


If you have not logged into the website then please enter your details below.



 

 

Popular this Month...

Our Top 10 most popular articles this month

 

Today's Picks...

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd

 


Promotions

 

Media Partners