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Live Cell Culture Under Flow

publication date: Aug 5, 2022
 | 
author/source: Thistle Scientific

live-cell-culture-under-flow

 

Working with flow cells, shear stress, adhesion assays? The ibidi pump system can help.

The ibidi pump system, combined with ibidi polymer channel slides, provides defined shear stress over long term cell culture periods. All available direct from Thistle Scientific.

 

live-cell-culture-under-flowHow does it work?

Applying pressured air to the reservoirs of the Fluidic Unit moves the medium and generates the flow in the µ-Slide channels. In order to avoid wasting any medium, the liquid is pumped back and forth between reservoir A and reservoir B by switching the valve V1.

To assure a unidirectional flow in the channel, the other valve V2 is switched simultaneously. As the tubing parts inserted in the valve V2 are criss-crossed, the medium is always directed to the same end of the channel slide.


Applications

  • Defined shear stress in long-term culture (e.g., endothelial cells, kidney, or biofilm)
  • Live cell imaging and immunofluorescence for analysing shear stress response
  • Mimicking shear stress conditions in microcapillary, venous, and arterial flow
  • Rolling and adhesion of suspended cells on substrates
  • Stop flow experiments
  • 3D cell culture: interstitial flow

 

 

ibidi Products for this Application

 

live-cell-culture-under-flowIbidi Pump System

Ideal simulation of various physiological conditions in blood vessels - a pump system for the cultivation of, e.g. endothelial, cells under flow. Compatible with ibidi heating and incubation systems as well as a range of channel slides.

 

 

live-cell-culture-under-flowibidi Channel Slides

Channel microscopy slides with a coverslip bottom (#1.5 ibidi Polymer or #1.5H glass); ideal for immunofluorescence, cell culture under flow, live cell imaging and high resolution microscopy on inverted microscopes.

 

Ibidi Pump Systems

ibidi Channel Slides

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