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Application Note - Fast UHPLC analysis of cell penetrating peptides

publication date: Apr 14, 2022
author/source: YMC Europe GmbH


Fast UHPLC Analysis of Cell Penetrating Peptides Using a YMC-Triart C18 column

Cell penetrating peptides (CPPs) are an interesting class of compounds since they are capable of transporting themselves across a cell membrane and also carry so-called “cargo” molecules with them. Due to their advantages over other transport mechanisms they are a focus for current research.

As CPPs often contain many basic amino acids, peak tailing can become a very difficult challenge. Due to the interaction with residual silanols the chromatographic results can be severely affected. Ideally, columns with a very well controlled endcapping are being employed. For this purpose, YMC introduced a multi- stage endcapping within their YMC-Triart column line.

The innovative phase modification prevents any tailing even for very basic substances! Download your copy of the new application note and see how sharp peak shapes can be obtained for the analysis of three CPPs in under four minutes.


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