In order to succeed in developing protein-based drugs, a fast and easy detection of alteration within the protein structure is necessary. In many cases, peptide mapping is the method of choice, where proteins are analysed after digestion. The small peptide units are examined using LC-MS. It is equally important to employ a powerful MS instrument together with a highly efficient high resolving column, as a result of, not only the large number of peptide subunit produced, but also the wide variety of modified structures which can occur.

All HPLC separations have been performed on a Jasco X-LC system. The gradient was formed from acetonitrile and water with TFA as an ion pair agent. The gradient conditions are described in figure 1.
The conventional HPLC method was carried out using a 150 x 4.6 mm ID column packed with 5-μm YMC–Pack Pro C18.
The conventional HPLC method described above was easily transferred to an ultra-fast method using a short YMC UltraHT Pro C18, in order to lower solvent consumption.

Click here to download the Ultra Fast LC Application Guide.

For further information please contact Dr. Friederike Becker, YMC Europe GmbH, Phone: +49 (0)2064 427-261, Fax: +49 (0)2064 427-222 or visit www.ymc.de