Channels

 

Special Offers & Promotions

 

 

Latest News

 

 

View Channel

New Products

 

 

View Channel

Video Presentations

 

 

View Channel

Separation Science

 

 

View Channel

Microscopy & Image Analysis

 

 

View Channel

Laboratory Automation & IT Solutions

 

 

View Channel

 

Ultra Fast LC for Peptide mapping

In order to succeed in developing protein-based drugs, a fast and easy detection of alteration within the protein structure is necessary. In many cases, peptide mapping is the method of choice, where proteins are analysed after digestion. The small peptide units are examined using LC-MS. It is equally important to employ a powerful MS instrument together with a highly efficient high resolving column, as a result of, not only the large number of peptide subunit produced, but also the wide variety of modified structures which can occur.

All HPLC separations have been performed on a Jasco X-LC system. The gradient was formed from acetonitrile and water with TFA as an ion pair agent. The gradient conditions are described in figure 1.
The conventional HPLC method was carried out using a 150 x 4.6 mm ID column packed with 5-μm YMC–Pack Pro C18.
The conventional HPLC method described above was easily transferred to an ultra-fast method using a short YMC UltraHT Pro C18, in order to lower solvent consumption.

Peptide Mapping

Click here to download the Ultra Fast LC Application Guide.

For further information please contact Dr. Friederike Becker, YMC Europe GmbH, Phone: +49 (0)2064 427-261, Fax: +49 (0)2064 427-222 or visit www.ymc.de


If you have not logged into the website then please enter your details below.



 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month

Top 10 most popular articles this month

 

 

Today's Picks

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


 

Media Partners

 

Exhibitions & Events