Channels

 

Special Offers & Promotions

 

 

Latest News

 

 

View Channel

New Products

 

 

View Channel

Video Presentations

 

 

View Channel

Separation Science

 

 

View Channel

Microscopy & Image Analysis

 

 

View Channel

Laboratory Automation & IT Solutions

 

 

View Channel

 

Tomocube adds 3D Fluorescence Imaging to Holotomography Microscopy for World First

publication date: Dec 5, 2018
 | 
author/source: Tomocube

CU-501


HT-2 microscope facilitates three-dimensional fluorescence and optical diffraction tomography of live cells with minimal stress on cells

The HT-2 from Tomocube is the world’s first microscope to combine both holotomography and 3D fluorescence imaging into one unit. Capable of simultaneously capturing high resolution 3D optical diffraction tomography and 3D fluorescence images, the new microscope enables long-term tracking of specific targets in live cells while minimising stress. The capability to easily deliver holotomography and fluorescence correlative analysis in 2D, 3D and 4D will enable researchers and clinicians to open new frontiers in bioscience and better understand, diagnose, and treat disease.

“Research and diagnosis in the life sciences depends on the information that can be found using cellular analysis,” says Aubrey Lambert, Tomocube’s Chief Marketing Officer. “Current microscopy techniques limit the quantity and quality of information available to researchers and clinicians and harm the living cells during long-term studies, but the HT-2 is set to overcome these limitations.”

The new microscope builds on the novel holotomographic technology first shown in the Tomocube HT-1, providing morphological, chemical and mechanical properties of cells through the 3D refractive index (RI) tomograms but adding molecular specificity information through the fluorescence imaging capability. The highly detailed fluorescence images show the position of specific target organelles or structures in living cells, while consecutive measurements of time-lapse 3-D RI tomography enables the monitoring of cells and their structures with minimal stress.

The HT-2 incorporates a customisable three-channel LED light source (385, 470 and 570 nm) and a motorized Z-drive with a step resolution of 150 nm to generate highly detailed Z-stack images. The TomoStudio software suite controls all the functionality of the HT-2 and provides fast imaging capability and 2D/3D/4D visualization of the cellular images based on 3D RI distributions of the cells and tissues. At its core is the same patented complex digital micromirror device (DMD) optical light shaper used in the Tomocube HT-1. Consisting of several hundred thousand micromirrors arranged in a rectangular array, the DMD eliminates the need for moving parts in the lightpath and delivers stable performance during long-term studies.

 

Benefits

  • Correlative microscopy in one instrument - HT-2 provides high-quality 3D images of both holotomography and 3D fluorescence for each sample.
  • Quantitative data marked with fluorescence - HT-2 provides morphological (volume, surface area, projection area, sphericity and ellipticity), chemical (dry mass, concentration) and mechanical (cell deformability) properties of cells with 3D refractive index (RI) tomogram. Moreover, fluorescence image provides information about molecular specificity.
  • Live cell molecular and holographic imaging with minimal stress on cells - Simultaneous measurement capability of time-lapse 3-D RI tomography and fluorescence image allows long-time tracking of specific targets in live cells. The fluorescence image provides the position of specific target organelles or structures in live cell, and consecutive measurements of time-lapse 3-D RI tomography enables the monitoring of cells and their structures with minimal stress.

 

more about the HT-2 microscope

more from tomocube



If you have not logged into the website then please enter your details below.



 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month

Top 10 most popular articles this month

 

 

Today's Picks

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


 

Media Partners

 

Exhibitions & Events