Follow us...

 

Search News Archives

Monthly Newsletter

 

Lab Bulletin May Newsletter

view the latest issue

Subscribe

News Channels

 

New Laboratory Products

 

Lab News

 

Microscopy & Image Analysis

 

Separation Science

 

Research & Case Studies

 

Literature

 

Videos

 

Events | Webinars

 

 

 

Conferences | Events

Fluorescence Detection Speeds Determination of Sialic Acids

Thermo Fisher Scientific is pleased to announce a new ultra high-performance liquid chromatography (UHPLC) method that allows high resolution of sialic acids in a 10 min analysis time and requires less organic solvent than other published methods.

UHPLC Determination of Sialic Acids with Fluorescence DetectionApplication Note 278: UHPLC Determination of Sialic Acids with Fluorescence Detection demonstrates that the sensitivity of fluorescence detection easily allows straightforward determination of sialic acids in the pmol range.

In AN 278, N-acetylated sialic acids are determined and O-acetylated sialic acids are identified by UHPLC with fluorescence detection following acid hydrolysis and 1,2-diamino-4,5-methylenedioxybenzene dihydrochloride (DMB) derivatization of glycoprotein samples. The described assay uses a short format Thermo Scientific Acclaim Rapid Separation LC (RSLC) 120 C18 column that allows fast run times (10 min) and requires less organic solvent compared to a commonly used 40 min method. The sensitivity of fluorescence detection easily allows determination of sialic acids that are present in the pmol range in glycoprotein hydrolyzates. This sensitivity allows for straightforward determination of Neu5Ac, Neu5Gc, and O-acetylated sialic acids in the derivatized samples.

This application note and many others can be found at www.thermoscientific.com/dionex under the Documents tab


 

 

Popular this Month...

Our Top 10 most popular articles this month

 

Today's Picks...

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd

 


Promotions

 

Media Partners