Recent Articles in Separation science - Articles and Application notes
Reversed-Phase HPLC: Columns, Dimensions and Testing Part 1: Column selectionMar 22, 2010
This article provides some general principles to apply when considering the purchase of HPLC columns for use in the laboratory. The types of data obtained from column characterisation experiments are highlighted and the relevance of the data to the intended application is discussed. Part 2 will focus on selection of appropriate testing regimes.
Read MoreDETERMINATION OF PACLITAXEL IN HUMAN PLASMA BY XLC-MS USING SYMBIOSIS PHARMAMar 21, 2010
The SymbiosisTM Pharma is Spark Holland's unique solution for integrated on-line SPE-LC-MS automation (XLCMS). The system offers large flexibility in processing different types of samples selecting one of the three fully automated operational modes: LC-MS, XLC-MS, or AMD (Advanced Method Development). A sensitive, specific, accurate and precise assay was developed for the on-line extraction and determination of paclitaxel in human plasma by XLC-MS, using the SymbiosisTM Pharma
Read MoreDetermination of Chloramphenicol in eggs by XLC-MS using the Symbiosis Pharma SystemMar 21, 2010
SymbiosisTM Pharma is Spark Holland's unique solution for integrated online SPE-LC-MS automation (XLC-MS). The system offers large flexibility in processing different types of samples selecting one of the three fully automated operational modes LC-MS; XLC-MS; AMD (Advanced Method Development). This application shows a fast method development for Chloramphenicol with the use of the AMD mode of Symbiosis Pharma together with the HySphereTM method development cartridge tray (Spark p.n.0722.650).
Read MoreUltra low level determination of 25-mono-hydroxy-vitamin D2 and D3 in human plasma by acetonitrile- free XLC-MS/MS using the Symbiosis SystemMar 21, 2010
A fast, sensitive and simple method has been developed for the analysis of 25-mono hydroxy vitamin D2 (MHVD2) and 25-mono hydroxy vitamin D3 (MHVD3) in human plasma.
Read MoreDETERMINATION OF PAH IN GROUND, SURFACE AND DRINKING WATER USING SYMBIOSIS ENVIRONMar 21, 2010
Polycyclic Aromatic Hydrocarbons (PAH), a group of chemically related compounds, are known for their potential carcinogenic properties. In the environment these compounds are formed during pyrolysis and incomplete combustion processes. They are found throughout the environment in air, water and soil. The (by law enforced) maximum acceptable concentration level of PAH in water is approximately 10 ppt for each individual component.
Read MoreBinary prep. HPLC-system for processing large volumes of ureacontaining protein solutionsMar 18, 2010
Often client specific requirements for multipurpose equipment can not be solved with “off the shelf“ solutions. In this specific case, the Kronlab custom build approach proved very useful: every component has been specifically approved for the application at hand and following a specified procedure it is integrated into an LC system. The result is a made-to-measure system.
Read MoreDionex Beverage Application Notebook Demonstrates Unique Analytical Solutions for Commonly Used BeveragesMar 17, 2010
The Dionex Beverage Application Notebook, a compilation of beverage applications in an easy-to-use PDF, is available on the Dionex website at www.dionex.com. The global beverage industry is growing each year with the introduction of new products, such as vitamin-fortified water, energy drinks, anti-aging water, and herbal nutritional supplements.
Read MoreInformative Report on Deep Well Microplate SampleFeb 18, 2010
A new technical report is available from Porvair Sciences Ltd. that investigates and demonstrates the importance of high quality raw material selection for the manufacture of deep well microplates.
Read MoreMelamine Contamination Resurfaces in China!Feb 9, 2010
The 2008 China melamine scandal in which the discovery of milk powder adulterated with melamine led to a global recall of Chinese infant milk formula, chocolates, biscuits, cakes, and other milk-based products is resurfacing. This month, the China Daily reports that melamine-contaminated milk-based products from three companies have been removed from shelves in the Guizhou province.
Read MoreDionex Applications for Securing Purity of Anti-Coagulant HeparinJan 15, 2010
Dionex Corp. is pleased to announce the availability of two application notes created in support of the official release of the United States Pharmacopeia (USP) stage 2 heparin sodium monograph, published October 1, 2009
Read MoreThe Bio-ChromatographersDec 30, 2009
Simply high dynamic binding capacities (DBC’s) are no longer sufficient. High DBC, at high flow rates with close to 100% recovery, across a wide range of molecular weights, this is what chromatographers look for in modern ion exchange resins. “BioPro” YMC’s hugely successful ion exchange material offers exactly this. The growing user base confirms very good separation, high dynamic binding capacities and excellent recoveries for the anion as well as the cation exchanger material.
Read MorePreheating of mobile phases - "nice-to-have" or "need-to-have"?Dec 30, 2009
Controlling the temperature of a column is a prerequisite for reproducible results in HPLC. Column ovens are part of most HPLC systems and many of them offer space even for semi preparative columns. But with increasing inner diameter the heat transfer from the air surrounding the column to the whole cross section of the column (filled with the cold mobile phase) becomes more and more difficult. This may result in bad peak shapes, as the radial temperature gradientcauses different separation conditions for every part of the sample depending on its distance to the outer diameter.
Read MoreHPLC-Tip: Degassing of the mobile phaseDec 30, 2009
A released gas in mobile phases during analysis causes noisy baselines and pressure fluctuations which affect sensitivity and reproducibility. It also severely compromises the use of fluorescence-, refractive-index- or UV-detectors (particularly at wavelengths below 210 nm). As a consequence effective mobile phase degassing must be employed for many analyses.
Read MoreUltra Fast LC for Peptide mappingDec 29, 2009
In order to succeed in developing protein-based drugs, a fast and easy detection of alteration within the protein structure is necessary. In many cases, peptide mapping is the method of choice, where proteins are analysed after digestion.
Read MoreFast routine analysis of alkaloidsDec 29, 2009
The method transfer from a conventional column to an ultra fast analysis for alkaloids has shown that the use of YMC-UltraHT 2 µm column and adjustment of flow parameters for the smaller column dimensions can reduce the analyses time by about 85% accompany with significant solvent savings and significant solvent savings whilst increasing the sample throughput in the laboratory.
Read MoreHPLC hint: The advantage of premixed solvents in HPLCDec 29, 2009
For several reasons the storage of 100% pure solvents in the reservoir bottles of an HPLC system can be a problem. Pure acetonitrile, in particular, affects the system by adsorption of polymers from transmission lines resulting in sticky surfaces that inhibit the opening and closing of the valves
Read MoreSimultaneous separation of pesticides by LC/MSDec 29, 2009
With the constantly rising population and frequent uncertain weather, the need for high yielding agriculture is paramount. This means that use of plant protection agents, such as herbicides and insecticides is necessary, in order to secure harvests.
Read MoreNormal Phase Chromatography Dec 28, 2009
Normal phase chromatography is not very popular with most analytical HPLC users. Poor reproducebility of the results is often cited as an argument against normal phase chromatography.
Read MoreFastLC Application for CurcuminDec 28, 2009
The plant pigment curcumin, found in the popular Indian curry spice turmeric, is well known as an antioxidant for foods. However, researches have paid attention to another characteristic. Curcumin is highly active against the growth of certain cancers.
Read MoreMelamine in pet-foodDec 23, 2009
Melamine (sometimes confused with the plant and animal compound, melanine or another compound, melanin) is an industrial chemical used in the manufacture of plastic products. Despite that, tests showed that a number of milk companies have used it in processing milk to artificially increase the apparent protein content by addition of non-protein nitrogen and so allowing illegal dilution of the product with water with a corresponding increase in sales value.
Read MoreDetection of Mycotoxins in NutsDec 21, 2009
Mykotoxins are natural metabolism products of molds which have a toxic effect on humans and animals. Just like micro-organisms which generate antibiotics, molds which generate mycotoxins have spread all over the world. Aflatoxins are the most toxic form of mycotoxins. Some types of food show an increased risk of aflatoxin release due to fungal infestation: dried fruit, spices, nuts (peanuts, hazelnuts, pistachios) and grain (wheat, corn).
Read MoreMycotoxins in NutsDec 11, 2009
Every year during the pre- holiday activities the use of nuts increases in order to prepare for the holiday baking. To a large degree unnoticed by consumers is the problem of a possible contamination of these foods with mycotoxins.
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