![Performing in vivo HTS assays using the Infinite M1000 Tecan Infinite M1000](/custom/Tecan Infinite M1000.jpg)
Tecan's Infinite
® M1000 multimode
microplate reader
has enabled scientists in the
Department
of Cellular Biology and Anatomy
at Georgia Health Sciences University
(GHSU), USA, to develop a high throughput system for reporter-based drug
screening assays in living zebrafish disease models
1. Dr Jeff
Mumm, Assistant Professor at GHSU, explained: "Quantitative
microplate reader systems have revolutionized the pace of drug discovery,
enabling the development of reporter-based
in vitro and
in silico
assays that allow high throughput screening (HTS). However, biological
validation has become a bottleneck in the drug discovery process, due to a lack
of HTS-compatible
in vivo assay platforms. Quantifying reporter levels
in living zebrafish provides a versatile means of alleviating this road block."
"The flexibility of the Infinite M1000 has allowed us
to develop a simple and cost-effective automated screening method based on
fluorescent or luminescent reporter detection in live zebrafish. Using this
method we have been able to quantify the loss and regeneration of targeted
cells in zebrafish disease models, as well as small molecule-induced changes in
disease-linked molecular signaling pathways. High signal to noise ratios
allow us to monitor changes in individual fish - which accounts for wide reporter
level variance across populations by normalizing signals to each individual's
ground state - enabling us to detect long-term changes over several
days. This powerful technique will benefit a wide variety of high
throughput chemical and genetic screens in live zebrafish, potentially
accelerating the validation of new drugs."
To
find out more on Tecan's Infinite M1000, visit
www.tecan.com/InfiniteM1000
Walker SL, et al. (2012) Automated Reporter Quantification
In Vivo: High-Throughput Screening Method for Reporter-Based Assays in
Zebrafish. PLoS ONE, published 04 Jan 2012.
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