Tecan’s new Rapid EZ DNA-Seq library preparation kit is designed to offer PCR-free generation of sequencing-ready libraries for next generation sequencing (NGS).

Ideally suited to whole genome sequencing and metagenomics for samples with varied GC content, as well as amplicon sequencing, this automation-friendly kit complements Tecan’s existing Celero™, Rapid and Ovation® Ultralow DNA-Seq library preparation solutions.

The Rapid EZ DNA-Seq library preparation kit is an end-to-end solution for PCR-free generation of NGS libraries in just 2.5 hours. This kit uses Tecan’s robust enzymatic DNA fragmentation and DimerFree® library construction technologies, eliminating the need for tedious optimization regardless of input amount. It offers a truly ‘add and incubate’ workflow, making it well suited to applications sensitive to PCR bias or PCR artifacts, such as microbiome and prokaryotic samples, or sequencing of GC- or AT-rich regions of the human genome.

The Rapid EZ DNA-Seq kit includes all of the reagents needed for library construction, with pre-plated Unique Dual Index (UDI) adaptors to enable flexible multiplexing and detection of ‘index hopping’. The simple, flexible workflow can be easily automated on Tecan’s advanced liquid handling platforms, such as the DreamPrep™ NGS workstation.

Rasmus Lind, General Manager of Tecan Genomics, commented: “Our portfolio of innovative DNA-Seq products uses robust chemistries to simplify library preparation, accelerate processing and improve the quality of sequencing data. With the development of the Rapid EZ kit, we now have solutions for every sample type – from very low to high throughput applications.”

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About Tecan

Tecan is a leading global provider of laboratory instruments and solutions in biopharmaceuticals, forensics and clinical diagnostics. The company specializes in the development, production and distribution of automated workflow solutions for laboratories in the life sciences sector. Its clients include pharmaceutical and biotechnology companies, university research departments, forensic and diagnostic laboratories. As an original equipment manufacturer (OEM), Tecan is also a leader in developing and manufacturing OEM instruments and components that are then distributed by partner companies. Founded in Switzerland in 1980, the company has manufacturing, research and development sites in both Europe and North America and maintains a sales and service network in 52 countries. In 2018, Tecan generated sales of CHF 594 million (USD 606 million; EUR 516 million). Registered shares of Tecan Group are traded on the SIX Swiss Exchange (TECN; ISIN CH0012100191).

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