Merck Millipore Introduces Magna ChIP® HiSens Chromatin Immunoprecipitation Kits  

• Enables enrichment from both low and high amounts of input chromatin
• Delivers low backgrounds and high signal-to-noise ratios for ultra-sensitive detection
• Suitable for a wide range of input amounts - from 10,000 to 1,000,000 cells 

Merck Millipore, the Life Science division of Merck, today introduced Magna ChIP® HiSens chromatin immunoprecipitation kits for superior enrichment and detection of specific regions of chromatin-associated DNA. These new kits enable target chromatin enrichment from both low and high amounts of input chromatin while delivering low backgrounds, high signal-to-noise ratios, and ultra-sensitive detection that is compatible with all commonly used downstream analysis applica­tions including qPCR, next generation sequencing, and microarray. 

Two versions of this new chromatin immunoprecipitation kit are available. The Magna ChIP® HiSens chromatin immunoprecipitation kit includes reagents, buffers, and beads for ChIP as well as materials for chromatin preparation and isolation from either cultured cells or tissues. The EZ-Magna ChIP™ HiSens chromatin immunoprecipitation kit includes positive and negative control antibodies and a validated qPCR primer set, in addition to the standard kit's components.

Unique to these kits is a single buffer system used for all sonication, ChiP, and wash steps. This streamlined approach, plus their specialized blend of protein A/G magnetic beads, not only allows ChIP from a wider range of antibody isotypes than kits with protein A or G beads alone, it also delivers lower backgrounds and higher fold enrichment.  Unlike other approaches the Magna ChIP® HiSens kits are designed to allow ChIP analysis by qPCR without additional clean-up, reducing the time required to perform the protocol along with eliminating the potential for sample loss during clean-up.

Using these kits, researchers can ChIP from a wide range of input amounts (10,000 to 1,000,000 cells or cell equivalents) and get excellent performance starting from either cells or tissues.  Performance of the Magna ChIP® HiSens kit was benchmarked against that of other low input ChIP kits. After strictly following the protocols for all kits evaluated, the Magna ChIP® HiSens kit showed significantly higher performance as measured by fold enrichment and background levels than competitor kits.

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