Special Offers & Promotions



Latest News



View Channel

New Products



View Channel

Video Presentations



View Channel

Separation Science



View Channel

Microscopy & Image Analysis



View Channel

Laboratory Automation & IT Solutions



View Channel


Field Flow Fractionation Improves Characterization of Nanoformulated Medicines

publication date: Mar 22, 2019
author/source: Postnova Analytics GmbH

Asymmetric Flow Field Flow FractionationPostnova Analytics reports on a recent independent review which details how Asymmetric Flow Field Flow Fractionation (AF4) is a valuable technique to improve the quality of nanoformulated medicines.

Currently, only a few standardized methods exist to characterize some of the crucial physicochemical properties and their impact on biological systems of medical nanoparticles. Unfortunately, they are often not applicable to the more complex and innovative nanoformulations.

Particle size and size distribution are key factors for the manufacturing quality, as well as the efficacy and safety of medicinal nanoparticles. Despite its widespread usage in the nanomedicine field, batch-mode dynamic light Scattering (DLS) is generally only considered a ‘low-resolution’ method for checking the integrity of monodispersed samples. To overcome the inherent difficulties that are imposed when analysing a polydisperse nanoformulated sample by DLS, a fractionation step should be incorporated prior to measurement. This can be done by using DLS and MALS detectors coupled online to a Postnova Analytics Asymmetric Flow Field Flow Fractionation (AF4) system.

In the independent report the authors describe how the USA Nanotechnology Characterization Laboratory (NCI-NCL), and more recently the European Union Nanotechnology Characterization Laboratory (EU-NCL), have developed standard operating procedure (SOP) to determine medicine nanoparticle size distribution by coupling AF4 with on-line size measurement (DLS and MALS). They state how the AF4-MALS-DLS technique as applied to medicine nanoparticle characterization might be considered as an alternative to the DLS alone for regulatory purposes.

This SOP was successfully applied by the EU-NCL consortium to characterize pristine medicine nanoparticles with the aim to resolve the particle size distribution of complex mixtures and discriminate between population of particles with different shapes. In addition, the SOP was used to discriminate between larger particles and small aggregates, and to monitor small changes of particle size distribution due to batch to batch variability or instability during long-term storage, all cases where batch-mode DLS fails. In addition, the EU-NCL also developed an SOP to study the nanoparticle behaviour in presence of serum proteins, which is crucial to understand the biological effects of medicine nanoparticles in vitro and in vivo.

The Postnova AF2000 is a high-performance Asymmetric Flow Field-Flow Fractionation (AF4) platform for separation of nanoparticles, macromolecules and proteins in complex matrices such as pharmaceutical formulations and foodstuffs. Modular in design, the AF2000 incorporates the combined experience, expertise and technological advances from Postnova Analytics' two decades of leadership in FFF. Incorporating a range of FFF modules in a single integrated system to provide universal separation, the AF2000 offers more flexibility, better performance and more robust results than any system before.


find out more

more news from this company

If you have not logged into the website then please enter your details below.


Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin


Request your free copies HERE




Popular this Month

Top 10 most popular articles this month



Today's Picks



Looking for a Supplier?

Search by company or by product


Company Name:






Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


Media Partners


Exhibitions & Events