Follow us on
Search News Archives
Conferences | Events
Monthly Newsletter
Lab Bulletin Media Partners

Determining the Physicochemical and Biophysical Attributes of Monoclonal Antibodies

publication date: Jun 25, 2018
 | 
author/source: Postnova Analytics GmbH

EAF2000 system


Postnova Analytics has published a new poster that compares the suitability of Electrical Asymmetrical Flow Field Flow Fractionation (EAF4) and Size Exclusion Chromatography (SEC) for determining the physicochemical and biophysical attributes of monoclonal antibodies.

In the described work, a reference monoclonal antibody (RM 8671 mAb), from the U.S. National Institute of Standards and Technology (NIST), was used to compare separation, aggregation quantification, and recovery parameters for an EAF4-UV-MALS versus SEC-UV-MALS techniques. The NIST mAb provides a representative test molecule for development of novel technology for therapeutic protein characterization. 

The poster describes how the EAF4 module enabled simultaneous measurement of size and surface charge characteristics (electrophoretic mobility) of antibodies and proteins. Further while no aggregates were detected by SEC, the FFF system showed protein / antibody aggregates represented 10% of the total mass injected. The researchers concluded that the FFF open channel design may allow for better recovery of injected mass than SEC which is particularly important when seeking to quantify aggregates present in small amounts.

Postnova Analytics EAF4 technology uniquely combines the principle of Electrical and Asymmetrical Flow FFF in just one system. In an EAF2000 system - Electrical and Cross Flow Fields are applied simultaneously across the FFF channel enabling separations by size and particle charge based on electrophoretic mobility. Combining these two powerful separation techniques in a single platform opens the door to characterising complex proteins, antibodies and viruses as well as environmental and charged nanoparticles or polymers that have proven intractable to other techniques. 


more news from postnova analytics



If you have not logged into the website then please enter your details below.



 

Popular this month


Win an INTEGRA VOYAGER adjustable tip spacing pipette


Leeds Teaching Hospitals NHS Trust Pathology Lab to digitally scan 100% of slides


Thermo Fisher Scientific Announces Collaboration to Simplify Analysis of Complex Therapeutic Proteins


FreeLIMS.org launches a free, Laboratory Information Management System


Mira P for the pharmaceutical industry: Instant onsite verification of raw materials


Take your microwell plate pipetting to the next level!


World’s first high throughput microplate reader for circular dichroism


Eppendorf Presents First Internally Developed CO2 Incubator


Three benefits of hiring freelance Scientists


 

Can't find what you are looking for?


Search by company or by product


Company Name:

Product:





Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd