Follow us...

 

Search News Archives

News Channels

 

New Laboratory Products

Lab News

What's going on at Lab Innovations

Research & Case Studies

Microscopy | Image Analysis

Separation Science

Brochures & Literature

Videos

Events | Webinars

 

 

Conferences | Events

Combination Technique Improves Polysaccharide Characterization

publication date: Aug 8, 2019
 | 
author/source: Postnova Analytics GmbH

Combination-Technique-Improves-Polysaccharide-Characterization


Postnova Analytics reports on a new method for the accurate molecular weight determination of complex polysaccharides (Chitosans) using asymmetric flow field flow fractionation (AF4) coupled with multi-angle light scattering (MALS) and differential refractive index (RI) detectors.

Historically the technique of choice for characterization of polysaccharides has been Size Exclusion Chromatography (SEC). However, for more complex polysaccharides, such as Chitosans, employing SEC techniques requires pre-filtration of samples to remove interfering aggregate fractions which is both time-consuming and can lead to considerable sample loss.

The described AF4-MALS-RI method allows the separation of polymer from molecular aggregates typically found in chitosan solutions making possible the accurate determination of molecular weight distribution of the polysaccharide macromolecules.

A new published paper** describes the effect of different experimental conditions on the obtained AF4 / MALS / RI results which were compared with those obtained from SEC-MALS-RI (Size Exclusion Chromatography-MALS-RI). The Chitosans analysed in this research were from different biological sources and of varying degree of acetylation.

The authors of the paper conclude that the AF4-MALS-RI method has the advantage of not only allowing the identification and separation of the aggregates present, but also the accurate determination of the MW distribution for a wide range of chitosan’s of varying type where results are well correlated with those obtained by more conventional techniques.

The Postnova AF2000 is a high-performance Asymmetric Flow Field-Flow Fractionation (AF4) platform for separation of nanoparticles, macromolecules such as polysaccharides and proteins in complex matrices. Modular in design, the AF2000 incorporates the combined experience, expertise and technological advances from Postnova Analytics' two decades of leadership in FFF. Incorporating a range of FFF modules in a single integrated system to provide universal separation, the AF2000 offers more flexibility, better performance and more robust results than any system before.

 

more news from postnova



If you have not logged into the website then please enter your details below.



 

 

Popular this Month...

Our Top 10 most popular articles this month

 

Today's Picks...

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd

 


Promotions

 

Media Partners