Search News Archives
Follow us on
Conferences | Events

Media Partners

 

ChIP-seq Assay Kit for Histone Methylation

publication date: Oct 3, 2016
 | 
author/source: Porvair Sciences Limited

Chromatrap® reports on the advantages of using its Chromatin Immunoprecipitation Sequencing (ChIP-seq) assay kits for histone methylation applications.

Chromatin Immunoprecipitation SequencingChromatrap’s® ChIP-seq kit uses solid state technology in parallel with high throughput sequencing to deliver a streamlined ChIP-seq protocol from small cell numbers and low chromatin concentrations to investigate the epigenetic landscape. Specifically adapted for broader chromatin concentrations, Chromatrap® ChIP-seq combines the dynamic range of Chromatrap® with the downstream analysis power of deep sequencing. This allows faster, more reproducible genome wide identification of  histone methylation sites. With no limitation in scale or resolution, Next Generation Sequencing can elucidate the role of epigenetic marks on gene transcription and epigenetic chromatin status.

The Chromatrap® ChIP-seq kit allows the user to perform up to 24 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations. The kit provides all of the major components required for performing ChIP assays to obtain high quality DNA for Next Generation Sequencing library preparation. With the Chromatrap® ChIP-seq kit you can sequence from as little as 1-50 µg of chromatin and perform up to 10 library preparations form a single IP. A complete ChIP-seq assay can be completed in just two days.

Launched worldwide in 2012 - Chromatrap® solid-state ChIP technology has been shown by a growing number of research groups worldwide to be more efficient than conventional bead-based methods. This is because the solid phase porous polymer, functionalized with either protein A or G, provides a greater surface area for chromatin antibody binding with very low non-specific binding. In addition, it uses a spin column approach, offering significant speed, process and carry-over advantages over sepharose or magnetic beads. DNA pull down with Chromatrap® is up to 25 times more than conventional methods, whilst the signal to noise ratio for DNA enrichment is 2 to 3 times better, even with low chromatin samples between 50ng to 3000ng per immunoprecipitation.


more about porvair


more news from porvair


 

 


If you have not logged into the website then please enter your details below.



 


Popular this Month

 

Win a PIPETBOY from the new Integra summer collection!

 

Great Savings off Selected GC Columns

 

CytoSMART raises 5.2 million Euros for further product innovation and growth

 

High Volume Sample Storage in Space Efficient 96-Well Format

 

Cherwell Publishes Guide on Sterility Testing

 

Effective Removal of DMSO from Stored Samples

 

ATL Webcast: A Practical Guide to Selecting the Right LIMS

 

Genetic study reveals how gut bacteria work to keep us healthy

 

Lonza Establishes Custom Cell Biology Services to Offer Unique Manufacturing and Testing Capabilities

 

Thermo Fisher Scientific Opens Food Safety Customer Solution Center in India

 

 


 

 


 

Can't find what you are looking for?

Search by company or by product


Company Name:

Product:


 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd