Thermo Fisher Scientific has developed a high-performance liquid chromatography (HPLC) method for a fast and sensitive determination of paraquat and diquat in tap and environmental water samples.
This approach uses on-line solid phase extraction (SPE) and achieves method detection limits that meet the requirements of both U.S. Environmental Protection Agency Method 549.2 and European Union 98/83/EC. Application Note (AN) 1051: Sensitive and Rapid Determination of Paraquat and Diquat in Tap and Environmental Water demonstrates use of a mixed-mode column to provide sufficient retention, excellent resolution, and good peak shape for these herbicides...
Thermo Fisher Scientific is pleased to announce a new technical note discussing rapid separation of inorganic cations in municipal wastewater using our high pressure capillary Ion Chromatography system, the Thermo Scientific Dionex 5000+ ICS HPIC system.
Technical Note 121: Fast Determinations of Inorganic Cations in Municipal Wastewater Using High-Pressure Capillary IC demonstrates how the separation of inorganic cations are important to municipal drinking water and wastewater treatment plants for compliance monitoring. Cationic determinations are necessary as part of the water monitoring program’s secondary water specification of acceptable taste....
Thermo Fisher Scientific is pleased to announce a new technical note on the fast separations of inorganic ions in saline solution samples.
TN 130: Fast Determinations in Salton Sea Samples Using a High-Pressure IC System demonstrates fast separations of inorganic anions and cations in a Salton Sea surface water sample and a neighboring municipal drinking water sample using ion-exchange chromatography. Monitoring salinity levels in estuarine ecosystems is critical to preventing salinity-specific diseases...
Thermo Fisher Scientific has developed an accurate and reproducible high-performance liquid chromatography (HPLC) method for the single-injection determination of tolterodine and tartrate—the active pharmaceutical ingredient (API) and its counterion—in an encapsulated drug product containing tolterodine tartrate.
Application Note 1047: Simultaneous Determination of Tartaric Acid and Tolterodine in Tolterodine Tartrate demonstrates that this method requires only 13 minutes per analysis. This new approach uses a trimode silica-based column and a detector that is ideal for the determination of counterions, many of which lack a chromophore. Pharmaceutical companies must ensure that a drug product contains the appropriate amount of its API. This is typically validated with an HPLC-based assay...
Malvern Instruments has published ‘Static Light Scattering Technologies For GPC/SEC Explained’, a valuable guide for anyone assessing the value of a light scattering detector for gel permeation/size exclusion chromatography (GPC/SEC).
Covering the theory and practice of molecular weight measurements using static light scattering, this basic guide serves as both an introduction for those with no prior knowledge of the technique and a reference document for anyone looking to enhance their understanding. The white paper is available as a free download from the Malvern Instruments website
Read MoreExtraction of Metaldehyde, Geosmin and 2-Methylisoborneol from WaterJul 16, 2013
Kinesis has published a new Application Note: Extraction of Metaldehyde, Geosmin and 2-Methylisoborneol from Water
The latest TELOS® SPE Application Note from Kinesis highlights the extraction of Metaldehyde, Geosmin and 2-Methylisoborneol from Water. Metaldehyde is a molluscicide whose use has markedly increased in recent years as a result of changing agricultural practices. Geosmin and 2-methylisoborneol (MIB) are naturally occurring alicyclic alcohols which possess potent earthy/musty taste and odour tainting properties. They can occur in raw and potable waters as metabolites derived from various micro-organisms...
Thermo Fisher Scientific has developed an ion chromatography method for nuclear power plants (NPPs) that determines trace concentrations (less than 1 µg/L) of fluoride, chloride, and sulfate in simulated secondary feed water that contains ethanolamine, hydrazine, and polyacrylic acid in just 20 minutes.
Application Brief (AB) 151: Determination of Trace Anions in Nuclear Power Plant Secondary Feed Water Containing Polyacrylic Acid demonstrates excellent retention time and peak area reproducibilities from 69 consecutive injections of a spiked simulated sample...
Thermo Fisher Scientific has developed an efficient high-performance liquid chromatography (HPLC) method using ultraviolet detection for the simultaneous determination in drinking water of all 19 phthalate compounds listed in key environmental regulatory documents.
Application Note 1045: Determination of Phthalates in Drinking Water by UHPLC with UV Detection demonstrates that the phthalates listed in European Union Directive 2005/84/EC, U.S. Environmental Protection Agency Methods 606 and 8061A, the Chinese HJ/T 72-2001, and the Standardization Administration of China GB/T 20388-2006 and GB/T 21911-2008 are well separated on a reversed-phase HPLC column in less than 25 minutes...
In a new paper published in Polymers & Polymer Composites (Vol 21, No 1, 2013), researchers at Kingston University in the UK report the results of continuing work to examine the effects of gamma irradiation on intraocular lenses (IOLs) used in cataract surgery. Triple detection gel permeation chromatography (GPC) using the Viscotek TDAmax system from Malvern Instruments was a key analytical tool in this work.
Baljit Ghatora and co-authors examined medical grade poly(methyl methacrylate) (PMMA), which is used especially widely in developing countries to make rigid intraocular lenses and for which low cost, convenient gamma irradiation is increasingly the preferred method for sterilisation. Previous reports have suggested that gamma irradiation has the potential to alter the structural features of the polymer, such as molecular weight and molecular size distribution, which in turn may impact the efficacy and performance of IOLs....
Read MoreJ G Finneran Associates introduces the 96-Well Multi-Tier Microtiter Plate System (Patented)Jun 25, 2013
An improvement on existing 96-Well systems, the Multi-Tier Plate is a modular, reusable system featuring a base plate capable of securing 0.5, 1.0, 1.5, or 2.0 mL conical glass, flat bottom and polypropylene vials.
The vented design of the base plate assures uniform heating and cooling when immersed in a water bath. The cover locks the liner in place and prevents vial pullout. The prescored PTFE/Silicone/PTFE liner permits easy needle penetration, while the Molded PTFE/Silicone liner locks into each of the 96 vials and reseals after multiple injections thus reducing the chance of solvent evaporation and cross contamination....
A new application note from Porvair Sciences details a protocol for its Chromatrap® ChIP assay kit that has been developed to enrich epigenetic marks from primary human tissue cells.
Chromatrap® Pro-A, the novel solid-based matrix for chromatin immunoprecipitation assays (ChIP) has been successfully used for isolation of high quality chromatin from difficult biopsy material. Focusing on an application to precipitate epigenetic marks from primary endometrial stromal cells - results show high abundant epigenetic marks from 1 million cells isolated from a tissue biopsy, with selective amplification of positive gene targets relative to negative IgG and negative gene loci....
Genevac, world leaders in solvent removal technology, has over 20 year’s experience of supplying evaporators for removal of solvents from oil, fat and fuel samples.
Oils, fats and fuels are normally isolated from natural sources using extraction or separation techniques that present the final sample in a solution of organic solvent. Before analysis such samples must be dried or concentrated. Whether extracting and recovering additives, evaluating lipid content of foods, characterising reclaimed cooking oils before reprocessing / conversion to biofuels, or investigating drillling test samples - oils, fuels and fats can often prove difficult to dry without using very high temperatures which may lead to loss of the more volatile fractions within the sample....
Read MoreDebunking Myths about Peak Area Response of Glycoprotein OligosaccharidesMay 20, 2013
Thermo Fisher Scientific has released a technical note (TN) that challenges the common misconception that there is significant variation in high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) peak area response of oligosaccharides.
TN 133: HPAE-PAD Peak Area Response of Glycoprotein Oligosaccharides summarizes the peer-reviewed scientific literature concerning this subject. This review concludes that the variation in HPAE-PAD oligosaccharide response with structure is minimal, especially among sets of similar oligosaccharides such as those found on many glycoproteins.....
RSA (Reduced Surface Activity) Glass vials are produced by a revolutionary new patent pending production process that virtually eliminates all silanols and produces glass which has a greatly reduced surface activity for basic compounds
RSA Glass vials are not coated or silanized. The lack of surface silanols make RSA Glass vials an excellent choice for many applications. If you are working with sensitive compounds commonly found in natural products, biological samples or chemical synthesis, the glass wall of your vial could be a problem for basic compounds. With RSA Glass vials this problem is eliminated....
Read MoreStandard-Compliant Method Determines Phenylurea Compounds in Tap WaterMar 19, 2013
Thermo Fisher Scientific has developed an efficient high-performance liquid chromatography (HPLC) method for the sensitive determination of nine phenylurea compounds in drinking water and commercial bottled beverages with method detection limits that meet those reported in U.S. Environmental Protection Agency Method 532 and concentration limits set in the European Commission’s Council Directive 98/83/EC
Application Note (AN) 1046: Determination of Phenylurea Compounds in Tap Water and Bottled Green Tea demonstrates that use of on-line solid-phase extraction(SPE) and ultraviolet detection eliminates the need for expensive and time-consuming off-line SPE. Phenylurea compounds are widely used as agricultural pesticides. Due to their slow degradation, they are frequently detected in surface waters at concentrations above 0.1 μg/L, which is higher than the European Commission’s drinking water limit often used as a quality standard for natural water...
A technical article from Genevac is available that details how its HT-24 centrifugal evaporation system is assisting a commercial flavours and fragrance company in its quest to find and isolate novel flavour ingredients from nature
Natural product derived discovery of novel flavour molecules is a highly complex process. Natural product sources yield complex extracts containing many components, which often do not lend themselves to processing and analysis. Conventionally, many steps of fractionation and evaluation are necessary until a compound is obtained from natural sources at the purity required for structural analysis. In the article a state of the art fractionation process using a Sepbox® Preparative HPLC - SPE system* and a Genevac® HT-24 centrifugal evaporator is described.
Read MoreKeeping sport clean with LoGiCal reference standardsJan 16, 2013
The Anti-Doping Control Laboratory at Barcelona’s Hospital del Mar Medical Research Institute (IMIM) is a world-recognized facility performing dope testing of athletes and animals for national and international sporting events, and was an early adopter of LGC Standards LoGiCal range of reference materials
Immaculada Figuera, a chemist in the laboratory, explained: “We test around 4,000 human and animal samples a year from sporting events around the world, screening urine, blood and serum samples for controlled substances. We perform a wide range of gas and liquid chromatography-based protocols, and use LoGiCal reference standards for both qualitative and quantitative analyses.”...
Read MoreSanofi-Aventis Puts A New Gas Chromatography Workhorse Through Its PacesJan 10, 2013
Thermo Fisher Scientific is pleased to announce a customer case study on the effective adoption of upgraded gas chromatography (GC) technology into an existing workflow with improved results and minimal effort
Dr. Dietrich Hilke, Head of Laboratory, Sanofi-Aventis, Deutschland GmbH, Frankfurt, and his team participated in the planning, testing, and evaluation of a new GC platform. Dr. Hilke’s laboratory analyzes 500 to 700 sample batches per year in a regulated environment for quality control of excipients, drug substances, and drug products. The team was able to directly transfer existing methods to the new GC system thus saving large amounts of time and effort....
Read MoreIon Chromatography: Metrohm publishes new Application Notes for the power industryNov 14, 2012
Metrohm publishes new Application Notes (ANs) on anion analysis in the power industry
In a recently published article, researchers from Novozymes Biopharma (Nottingham, UK) and Malvern Instruments (Malvern, UK) describe collaborative work using light scattering techniques to demonstrate the short- and long-term stability of the novel recombinant human albumins Albucult® and Recombumin®
These rAlbumins have been developed and optimized to deliver a safe, stable and regulatory-compliant product for therapeutic formulations. The teams applied the resolving and detection capabilities of size exclusion chromatography light scattering (SEC-LS) and dynamic light scattering (DLS) offered by Malvern systems to monitor the stability of these Novozymes products. The article ‘Tools for evaluating the stability of human recombinant albumins used in human therapeutics’ can be accessed at...
New Zetasizer Nano ZSP offers emerging rheology technique for weakly structured fluids
With the recent launch of the new top of the range Zetasizer Nano ZSP system, Malvern Instruments has added dynamic light scattering (DLS) Microrheology to its portfolio of materials characterization techniques. To introduce this powerful technique, the company has published an in-depth paper that explains the background to microrheology, and how it can be used to investigate the rheological properties of even the most weakly structured fluids, using sample volumes on the microliter scale. ‘An Introduction to DLS Microrheology’ includes data for protein (bovine serum albumin (BSA)) and polyethylene oxide (PEO) solutions that illustrate the significance and usefulness of the method. It is freely available from the Malvern website.
Thermo Fisher Scientific is pleased to announce a direct-detection reversed-phase high-performance liquid chromatography (HPLC) chromatographic assay method that separates the congeners in gentamicin sulfate and gentamicin sulfate-containing ointments, solutions, and creams in just 15 minutes
Application Note 1005: Gentamicin Sulfate Assay by HPLC with Charged Aerosol Detection demonstrates that this approach is faster than previously published methods, with excellent separation between the gentamicin congeners and resolution of many impurities that can be present in fermentation-manufactured products....