Thermo Fisher Scientific has developed an ion chromatography (IC)-based method to simultaneously determine lithium, sodium, and calcium in lithium carbonate without handling hazardous reagents.

Application Note 1090: IC Assay for Lithium, Sodium, and Calcium in Lithium Carbonatedemonstrates accurate determinations of these three analytes by separation on a cation-exchange column and detection by suppressed conductivity. This approach offers a fast (25 minutes), simple, accurate, and robust alternative to the time-consuming assays for these three analytes in the current United States Pharmacopeia (USP) monograph...

Thermo Fisher Scientific has developed a method that uses solid-phase extraction (SPE) well plates to improve sample preconcentration prior to analysis while maintaining high levels of precision, accuracy, recovery, and sample cleanliness.

Application Note 20941: SOLAμ for Pre-Analysis Sample Concentration demonstrates the ability to decrease the lower limit of quantitation by a factor of 20 without the need for lengthy evaporation procedures that may compromise analytical results. By performing a preconcentration step during sample preparation, analysts can reduce workflow, provide stability for analytes susceptible to loss or degradation during evaporation/reconstitution, and...

Thermo Fisher Scientific has developed an efficient high-performance liquid chromatography method that uses fluorescence detection to achieve a rapid and sensitive determination of rhodamine B in cosmetics.

Application Note 1102: Rapid and Sensitive Determination of Rhodamine B in Cosmetics demonstrates the advantages of this method using a rapid separation liquid chromatography (RSLC) column on an RSLC system. This approach provides good method reproducibility (retention time reproducibility relative standard deviation [RSD] of 0.09% and peak area reproducibility RSD of 0.75%) and a wide linear calibration range (from 2 to 1000 μg/L)...

Thermo Fisher Scientific has developed a precise, automated gas chromatography (GC) method for static headspace analysis of residual solvents in flexible packaging, using a headspace autosampler capable of multiple headspace extraction (MHE).

Application Note 10396: Static Headspace Analysis of Residual Solvents in Flexible Packaging and Quantitation with Multiple Headspace Extraction Following EN 13628-1: 2002 demonstrates the combined analytical power of the Thermo Scientific TRACE 1310 GC and the Thermo Scientific TriPlus 300 Headspace Autosampler to detect and quantify solvents that may leach from adhesives in flexible plastic packaging into the food products the packaging was...

Thermo Fisher Scientific has developed an efficient ion chromatography (IC) method with on-line sample cleanup for the rapid and sensitive determination of p-toluenesulfonic acid in water-insoluble drugs.

The on-line cleanup step provides the benefits of efficient matrix elimination, time savings, and convenience. Application Brief 165:Determination of p-Toluenesulfonic Acid in Water-Insoluble Drugs shows that this approach—performed on an integrated IC system that includes sample preparation capability and uses an ion chromatography column designed for separation with sodium carbonate/sodium biocarbonate eluents—demonstrates good linearity with a...

Thermo Fisher Scientific has developed a high-resolution hydrophobic interaction chromatography (HIC) method that can separate a fusion protein from a truncated version and a structural isomer.

Application Brief 171: High-Resolution Separation of a Fusion Protein demonstrates that two different HIC columns—the Thermo Scientific^™ ProPac^™ HIC-10 column and the new MAbPac^™ HIC-10 column—can each perform this challenging separation. While the ProPac HIC-10 column is an established column for the analysis of monoclonal antibody post-translational modifications, the MAbPac^™ HIC-10 column exhibits the best performance....

Thermo Fisher Scientific has developed two rapid and robust high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) methods for the accurate determination of common sugars in acid-hydrolyzed wood samples.

Application Note (AN) 1089: Determination of Carbohydrates in Acid Hydrolysates of Wood demonstrates that this approach achieves high-resolution separation of even the sugar pair rhamnose-galactose that has been challenging to resolve using existing HPAE-PAD methods. Both methods use a new polymeric column, the Thermo Scientific^™ Dionex^™ CarboPac^™ SA10-4μm column, configured with a new system, the...

Thermo Fisher Scientific has updated an ion chromatography (IC) method to more easily determine total choline (free and bound forms) in 17 samples representing all forms of infant formula, adult nutritionals, and pediatric formulas (powder, ready-to-feed products, and liquid concentrates).

Application Update (AU) 193: Choline in Infant Formula and Adult Nutritionals, a Single Laboratory Validation demonstrates that this simple yet robust approach decreases sample preparation time while accurately determining choline in these products. The method detailed in AU 193 runs on an IC system that requires only a source of degassed DI water for generation of high-purity eluent, thus simplifying operation while increasing precision and...

Thermo Fisher Scientific has developed a simple, efficient, sensitive, and reproducible capillary ion chromatography (IC) method for determining thiosulfate and pyrophosphate in crayfish wash powder.

Application Note 1088: Determination of Thiosulfate and Pyrophosphate in Crayfish Wash Powder demonstrates that this application is easily executed using a Reagent-Free^™ IC system capable of high-pressure IC controlled by data management software. The analyst simply adds water and samples to separate thiosulfate and pyrophosphate on a specialized anion-exchange column, the Dionex IonPac AS19 capillary column, thus...

Thermo Fisher Scientific has developed a simple, rapid, reliable, and accurate high-performance liquid chromatography (HPLC) method for the simultaneous determination of amlodipine and besylate in an amlodipine besylate drug substance.

Application Note 1087: Simultaneous Determination of Amlodipine and Its Counterion Besylate by HPLC demonstrates that this method requires only 8 minutes per analysis using a column designed for pharmaceutical analysis and ultraviolet detection. In addition, combining the analysis of the active pharmaceutical ingredient (API) and counterion into a single method saves times, reduces mobile phase consumption, and minimizes waste. Amlodipine besylate is a...

Thermo Fisher Scientific has developed a high-performance anion-exchange chromatography with pulsed amperometric detection method, based on AOAC Official Method 2011.18, to determine free and bound myo-inositol in infant formula and adult nutritionals.

Application Note (AN) 1083: Determination of Myo-Inositol (Free and Bound as Phosphatidylinositol) in Infant Formula and Adult Nutritionalsdemonstrates a column-switching technique used to remove strongly retained carbohydrates in the sample, thereby reducing run time. Pulsed amperometric detection with a gold on polytetrafluoroethylene disposable electrode offers high sensitivity while eliminating the need for sample derivatization and electrode polishing...

Thermo Fisher Scientific has demonstrated that a hydrophobic interaction chromatography (HIC) column, the Thermo Scientific^™ ProPac^™ HIC-10 column, is suitable for separating low-concentration species of an intentionally oxidized MAb from the native MAb.

The same method can be used to separate the papain fragments from the intentionally oxidized MAb, as well as the papain fragments of the native MAb. Application Note 1084: Separation of Papain Digests of Oxidized Monoclonal Antibodiesdetails how the ProPac HIC-10 column can be used to resolve antigen-binding fragments (Fab) and nonantigen-binding fragments (Fc) generated by papain digestion, as well as oxidized Fab/Fc from native Fab/Fc...

Thermo Fisher Scientific has developed a fast and robust ion chromatography (IC)-based method for the determination of chloride and sulfate in adenosine.

Application Note 1075: Ion Chromatography Assay for Chloride and Sulfate in Adenosine demonstrates that this method supports high sample throughput with a run time of just 3.5 min, requires no toxic chemicals, and can replace the U.S. Pharmacopeia monograph’s existing turbidity-based assays of these compounds. A high-pressure IC system (Thermo Scientific^™ Dionex^™ ICS-5000⁺ HPIC^™ IC system) combined with a...

Thermo Fisher Scientific has developed a high-performance liquid chromatography method to determine 20 polycyclic aromatic hydrocarbons (PAHs) in environmental waters using on-line solid-phase extraction (SPE) for sample preparation.

Thermo Fisher Scientific has developed a new ion chromatography (IC)-based method that overcomes typical challenges associated with quantification of low amounts of ammonia in the presence of a high concentration of sodium in sodium bicarbonate.

Application Note 1073: Determination of Ammonia in Sodium Bicarbonatedemonstrates that this assay meets the analytical performance characteristics recommended by United States Pharmacopeia (USP) General Chapter <1225>, including precisions (retention time relative standard deviation [RSD] less than 0.1% and peak area RSD less than 2%), accuracy (average recovery 88–117%), limits of detection, and robustness...

Thermo Fisher Scientific has developed an on-line solid-phase extraction–high-performance liquid chromatography (SPE-HPLC) method combined with ultraviolet (UV) absorbance detection for the simple and sensitive determination of tigecycline in a cell lysate sample.

Application Note 1074:Determination of Tigecycline in a Cell Lysate demonstrates that this approach, performed on a rapid separation LC (RSLC) system controlled by data management software, detects trace amounts of tigecycline in tigecycline-treated cells, concentrations so low that they cannot be assayed using a routine HPLC-UV detection method. The prevalence of multidrug-resistant bacteria in hospitals underscores the need for...

Thermo Fisher Scientific has developed a high-performance liquid chromatography (LC) method that simplifies determination of clenbuterol, ractopamine, and phenolethanolamine in meat using on-line solid-phase extraction (SPE) and ultraviolet absorbance detection.

Application Brief (AB) 154: Rapid and Sensitive Determination of Clenbuterol, Ractopamine, and Phenolethanolamine in Meatdemonstrates that this method, performed using a dual rapid separation LC system controlled by data management software, achieves method detection limits equivalent to those obtained by mass spectrometry (MS). Additionally, this approach offers the advantages of...

Magritek, a leading worldwide provider of compact NMR and MRI instruments, have released three new applications notes which showcase the use of their Spinsolve Benchtop NMR Spectrometer. 

Magritek has released three new applications notes to illustrate the versatility of their Spinsolve family of cost-effective benchtop NMR spectrometry solutions. Spinsolve uses standard 5 mm OD NMR test tubes, exactly the same as high field instruments, so sample handling is familiar and convenient. Spinsolve can be deployed in the lab where immediate results may now be obtained...

Cogent TYPE-C silica LC phases from MicroSolv Technologies have the ability to retain polar solutes at high concentrations of organic solvent by aqueous normal-phase (ANP) and non-polar compounds under reversed-phase (RP) conditions.

Thermo Fisher has developed an efficient and simple high-performance liquid chromatography (HPLC) method with ultraviolet detection for the determination of phenolic compounds (gallic acid, caffeic acid, 4-hydroxybenzoic acid, syringic acid, ferulic acid, salicylic acid, phloroglucinol, cinnamic acid, catechin, quercetin, vanillin, phloridzin, and phloretin) in apple orchard soil.

Application Note (AN) 1077: Determination of Phenolic Compounds in Apple Orchard Soil demonstrates that the entire analysis process, including sample preparation and separation for one soil sample, can be accomplished within 80 minutes using this method combined with an accelerated solvent extractor....

Thermo Fisher Scientific has developed a method for quantitative determination of over 200 multi-residue pesticides present in a blend of herbs and spices known as “churna”and widely used in Ayurvedic medicine using gas chromatography-tandem mass spectrometry (GC-MS/MS).

Application Note 10361, Analysis of Multi-Residue Pesticides Present in Ayurvedic Churna by GC-MS/MS describes a sensitive and precise assay for the detection of pesticides. Trace levels of the multi-residue pesticides were detected in the highly complex churna extracts at concentrations as low as 2.5 ng/g. The analysis was performed using a Thermo Scientific™ TRACE™ 1310 GC coupled to a Thermo Scientific™ TSQ 8000™ triple-quadrupole GC-MS/MS.

Thermo Fisher Scientific has released a new technical note which demonstrates the complete recovery of human hormones from drinking water.

Technical Note 148: Automated Extraction and Determination of Human Hormones in Drinking Water uses our newly released polymeric sorbent extraction cartridges (Thermo Scientific™ Dionex™ SolEx™ SPE HRPHS cartridges) on our solid-phase extraction instrument (Thermo Scientific™ Dionex™ AutoTrace 280 instrument) for the determination of human hormones. The SPE instrument can reduce solvent and labor costs by up to 90% in sample preparation...