Providing fast and accurate gene assembly

The latest issue of Integrated DNA Technologies' (IDT's) DECODED newsletter highlights how New England Biolabs (NEB) researchers were able to expe­dite the cloning of a 2.1 kb gene into a linearized pET21-a bacterial expression vector using 5 gBlocks^TM Gene Fragments that spanned the entire 2.1 kb sequence. Cloning, sequencing, and characteriza­tion of protein activity took less than 4 days, shorter than necessary for a third party to synthesize the complete gene and provide a finished construct.

Peichung Hsieh, Application Scientist at New England Biolabs, Inc. commented "The use of gBlocks Gene Fragments was simple and cost-effective, saved time, and resulted in fewer errors than the assembly of DNA from single-stranded syn­thetic oligonucleotides. These benefits ensure that Gibson Assembly cloning using gBlocks Gene Fragments will be a method we will continue to work with in the future".

gBlocks Gene Fragments are designed to reduce the time, error rate, and costs associated with producing single gene constructs or multiple genes within metabolic pathways. Used in conjunction with the recently intro­duced NEB Gibson Assembly^TM Master Mix, NEB reduced both the hands-on time and the total turnaround time of some of their cloning workflows.

You can read more about NEB's protocol in the latest issue of the IDT quarterly newsletter, DECODED. For more information about the complete IDT product portfolio, and to sign up to receive DECODED, visit www.idtdna.com.
 

About IDT

Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the United States, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; and Leuven, Belgium.