New application note gives researchers insights and guidance on maximizing HDR-mediated insertion of short fragments in CRISPR genome editing

As an advocate for the genomics age, Integrated DNA Technologies (IDT), has published a new application note on optimization of CRISPR-Cas9 for homology-directed repair (HDR) applications. Available as part of the IDT collection of CRISPR application notes and protocols, this article supports researchers using CRISPR in their genome editing studies to maximize HDR rate in knock-in and other short-insertion experiments.

Described as ‘true genome editing’, HDR is the most precise and error-free repair process available for breaks introduced in CRISPR experiments. However, due to the generally low baseline rate of HDR, optimizing reagents and experimental conditions becomes critical to maximize HDR-mediated insertions. This application note offers practical advice from IDT genomics experts, based on thorough optimization studies. This includes experiment planning, sequence design, preparation of reagents, delivery of CRISPR components, and verification of inserts.

This new resource adds to IDT’s extensive and continually updated library of genomics topics and underlines IDT’s commitment to developing new tools and techniques for genome editing and supporting scientists with up-to-date educational content for success in their work.

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