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Chromatrap Pro-A Spin Columns Improve ChIP Assay Performance

Chromatrap™ Pro-A Spin Columns Porvair Filtration Group has announced the launch of the first product, a ChIP kit containing 24 assays, based upon its novel ChromatrapTM Pro-A Chromatin ImmunoPrecipitation (ChIP) technology. The exciting new product has been enthusiastically tested at 12 beta sites around the world with excellent results and feedback.

ChromatrapTM is a new technology that offers a quicker, easier and more efficient way of undertaking chromatin immunoprecipitation assays. The new kits use revolutionary ChromatrapTM Pro-A spin columns which contain discs of an inert, porous polymer to which protein A has been covalently attached. During an assay the chromatin/antibody complex is retained by the disc. Flushing with three buffers and an elution step are all that is required to obtain the DNA fragments of interest.

In independent testing ChromatrapTM demonstrated levels of DNA pull down up to 25 times higher than with traditional bead based procedures, excellent DNA enrichment with signal-to-noise ratios typically 2 to 3 times better than competing procedures and top performance even with samples with as few as 15,000 cells per assay.

The new ChromatrapTM spin column approach offers significant advantages compared to methods based on sepharose or magnetic beads, which involve many steps of separation, pipetting and re-suspension. The BioVyonTM protein A discs used in ChromatrapTM spin columns have a proprietary internal structure, composed of pores that have inner surfaces specifically designed to maximize chromatin capture efficiency. Porvair's proprietary disc base material is chemically inert, reducing incidences of non-specific binding. All chromatin capture reactions take place inside the rigid porous disc; therefore there is no need for the many repetitive stages of suspension, collection and resuspension by pipette. Additionally no pre-blocking step is required. The Chromatrap spin column protocol allows washing steps that are fast, efficient and less prone to error and the 'flow through' characteristics of the column ensure excellent sample mixing and washing during the assay.

For further information please visit Porvair Filtration Group at the Epigenetics World Congress , www.chromatrap.com or contact the company on +44-1489-864330 /  kevin.quinlan@chromatrap.com.

Porvair Filtration Group is an international leader in the development and supply of materials and products for filtration and separation applications. Their expertise in a wide range of media and the dedication of their design, manufacturing, sales, test and research teams ensure they are at the forefront of filtration technology, delivering world class performance to the Life Science, Aerospace, Defence, Nuclear, Energy, Chemical Process, Industrial Process, Water Treatment and Printing  markets. With offices and manufacturing sites located in the UK, Europe and the USA combined with a world-wide network of market representatives and distributors, they pride themselves on their continuous innovation and research to meet global demands.


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