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Arcticzymes launches Heat&Run RNA sample prep kit


New heat-labile enzyme addresses major need in qRT-PCR and HTS markets

ArcticZymes AS today announced the launch of Heat&Run®, a new RNA sample prep kit based on the company's proprietary Heat-Labile (HL) dsDNase enzyme. The heat lability and other properties of dsDNase enable Heat&Run® to overcome the assay-critical limitations of existing DNase-based techniques by rapidly and completely removing genomic DNA (gDNA) without damaging the delicate RNA. With a simple three-step mix-heat-run protocol, Heat&Run® is also designed for seemless integration into existing laboratory workflows.

According to Managing Director Jan Buch Andersen, Heat&Run® addresses a major market need in both qRT-PCR and high-throughput sequencing: "Removing gDNA with current DNase-based techniques is extremely time-consuming, involving multiple protocol steps and increasing the risk of sample degradation. This is particularly a problem in the quantification of low-copy transcripts or small samples. By contrast, the heat-inactivation of HL-dsDNase has been shown to be gentle enough to preserve your entire RNA population, including small RNA species. In addition, you no longer need to use labour-intensive resin columuns, which are also hard to operation with small volumes. Similarly you can avoid adding EDTA that may interfere with your reactions downstream."

The Heat&Run® kit comprises both buffers and HL-dsDNase and is suitable for use with any sample material. The Heat&Run® kit represents the first of a new range of kits to be launched from ArcticZymes based on the company's proprietary enzymes isolated from Arctic sources.

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