Yourgene’s LightBench® instrument offers precise and accurate size selection of PacBio® SMRTbell® sequencing libraries increasing HiFi sequencing yields

Yourgene Health (part of the Novacyt group of companies), a leading international molecular diagnostics group, and PacBio, a leading developer of high-quality, highly accurate sequencing solutions, has announced a collaboration to optimise size selection for long-read sequencing workflows. PacBio has qualified Yourgene’s LightBench® instrument for size selection of long DNA fragments, enabling high yields for HiFi sequencing data.

Yourgene’s LightBench is an automated electrophoresis instrument, powered by Ranger® Technology. This platform uses a patent-protected, machine-vision algorithm to interpret and control gel electrophoresis in real time. It delivers accurate size selection for human genomic DNA and includes the ability to perform fragment length analysis for additional quality control.

PacBio HiFi sequencing is a single-molecule real-time (SMRT®) sequencing technology. It produces long (1,000 to 20,000+ bases), accurate reads with uniform genomic coverage and native methylation detection for use in applications such as whole genome reconstruction, haplotype phasing, detection of large and complex variants, and epigenetics.

A new Technical Note has been released for PacBio customers titled “Size selection of PacBio SMRTbell® libraries with the LightBench® instrument from Yourgene Health.” The Yourgene LightBench instrument is compatible with the Revio system. The automated LightBench workflow optimises walk away time, offering a scalable size selection solution for the preparation of PacBio SMRTbell sequencing libraries. Results from the initial study assessing two fragment lengths in 84 human genomic DNA samples from frozen blood for library preparation showed consistent yields for 8 kb or 10 kb size and a zero percent failure rate.

Matthew Nesbitt, Co-Founder of Ranger Technology, at Yourgene Health, said: “PacBio HiFi sequencing technology is a game changer for whole genome applications. High-resolution size selection is crucial to maximising data yields from long-read sequencing and Yourgene is proud to offer researchers a precise and scalable solution with the LightBench.”

Download the “Size selection of PacBio SMRTbell® libraries with the LightBench® instrument from Yourgene Health.” Technical Note to learn more.

About Yourgene Health

Yourgene Health is an international integrated technologies and services business, enabling the delivery of genomic medicine. The Group works in partnership with global leaders in DNA technology to advance diagnostic science. Yourgene Health is part of the Novacyt group of companies.

Yourgene primarily develops, manufactures, and commercialises simple and accurate molecular diagnostic and screening solutions, for reproductive health and precision medicine. The Group’s portfolio of in vitro diagnostic products includes non-invasive prenatal tests (NIPT) for Down’s Syndrome and other genetic disorders, Cystic Fibrosis screening tests, invasive rapid aneuploidy tests and DPYD genotyping assays.

Building on our expertise in genomic technology, Yourgene’s Ranger® Technology offers next generation size selection with a range of sample preparation platforms for dynamic target enrichment. Ranger® Technology can be utilised to improve workflows and performance in multiple applications including NIPT, oncology, infectious disease testing and gene synthesis.

Yourgene Genomic Services offers a clinical service from the UK focusing on precision medicine and reproductive health, including NIPT.

Yourgene Health is headquartered in Manchester, UK with offices in Singapore, the US and Canada.