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Analysis of Carbohydrates in Coffee in 10 mins

Application Note 280: Carbohydrate in CoffeeThermo Fisher Scientific is pleased to announce a new approach using high-performance anion-exchange (HPAE) chromatography with pulsed amperometric detection (PAD) for the determination of carbohydrates in extracts from instant coffee and green coffee beans. Application Note 280: Carbohydrate in Coffee: AOAC Method 995.13 vs a New Fast Ion Chromatography Method compares two methods: the Association of Analytical Chemists ( AOAC) official method 995.13 and a fast method using the CarboPac TM SA10 column . The former method has a longer run time (80 minutes) compared to the fast method (10 minutes). For certain sugars that may be difficult to resolve with the official method, minor modifications are suggested. The fast method, proposed for analyzing coffee carbohydrates, resolves 7 of the 11 coffee carbohydrates in 8 minutes and needs only the addition of deionized water for continuous operation.

Both methods have high precision and acceptable recovery for the carbohydrates in instant and green coffee extracts. In addition, disposable gold working electrodes provide consistently high detector response for both methods, assuring greater instrument-to-instrument and lab-to-lab reproducibility. In summary, both the AOAC official method 995.13 and the fast method are sensitive, accurate, reliable, and differ primarily in their total analysis time and peak resolutions for coffee carbohydrate determinations.

This application note and many others can be found at under the Documents tab.

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of $12 billion, we have approximately 39,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through three premier brands, Thermo Scientific, Fisher Scientific and UnityTM Lab Services, which offer a unique combination of innovative technologies, convenient purchasing options and a single solution for laboratory operations management. Our products and services help our customers solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity.

All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

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