Recent Articles in General Laboratory Articles and Application Notes
CHN Microanalysis: A Key Technique for Sample CharacterisationJun 13, 2011A white paper written by Exeter Analytical describes how CHN microanalysis provides a powerful and straightforward method for determining whether a sample is pure - a key aspect of sample characterisation. The paper discuss how by providing a precise and accurate analysis of the percentage Carbon, Hydrogen and Nitrogen in an organic sample - CHN microanalysis can compliment techniques such as Mass Spectrometry (MS) and Nuclear Magnetic Resonance (NMR) Spectrometry, thus enhancing the reliability of your analytical results...
Read MoreHuman DNA Experiment Uses 200k Digital Array ChipJun 7, 2011Fluidigm Europe has announced a new technical report that describes experimentation on a normal human genomic DNA sample using a prototype 200k Digital Array Chip on a modified BioMarkTM System.
Read MoreNew Application Note from BMG LABTECH - "Quantitative, High- Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability"Jun 2, 2011A Schistosoma viability assay (96 and 384 well format) is introduced that utilizes two fluorophores. Fluorescein diacetate emission is related to living cells while propidium iodide emission indicates the number of dead cells. This assay abandons low throughput microscopy and was performed in bottom reading mode on the POLARstar Omega microplate reader from BMG LABTECH...
Read MoreNew Application Note from BMG Labtech "The ELISAONE assay performed on the POLARstar Omega"May 9, 2011Traditional ELISA assays are known for their several wash steps in order to remove unbound antigen from the well. Here we introduce a fluorescence based ELISA assay that only needs one wash step. This technology is called ELISAONE™ and was developed by TGR BioSciences. The ELISAONE™ assay is both robust and sensitive as evidenced by EGF, TNFa and IL-2 data obtained with the POLARstar Omega microplate reader from BMG LABTECH...
Read MoreStraightforward monitoring of uranium in drinking and mineral waterMay 5, 2011Determining trace levels of uranium in drinking and mineral water by inductively coupled plasma mass spectroscopy (ICP-MS) requires expensive analytical instrumentation. In contrast, adsorptive cathodic stripping voltammetry (AdCSV) using Metrohm’s 797 VA Computrace is a straightforward, much less expensive alternative allowing measurements with superior sensitivity...
Read MoreNew Application Note from BMG Labtech "Thioflavin T aggregation assay to determine antibody stability"Apr 26, 2011The efficiency of antibody molecules is dependent on their stability. Stress can cause denaturation and aggregation resulting in inactive proteins. We introduce an assay setup where stability of antibodies is measured. Shaking stress is initiated by the FLUOstar Omega microplate reader and aggregation propensity of antibody candidates is measured with the help of a fluorescence based assay in 96-well format...
Read MoreNew Application Note "AlphaScreen Surefire" from BMG LABTECHApr 15, 2011GPCRs are a large family of receptors which have a broad involvement in cellular responses affecting many important body functions. An approach to measure Gi and Gq coupled receptors is the utilization of the ERK phosphorylation cascade. In this application note the POLARstar Omega microplate reader from BMG LABTECH is used to detect ERK phosphorylation using an AlphaScreen® Surefire® proximity-based assay in 384-well format...
Read MoreThermometric titration Apr 6, 2011No sample preparation required. Accurate results in one minute. No matrix effects and no cumbersome sensor-management necessary. Sounds too good to be true? Think again! Metrohm is pleased to introduce a new method for the straightforward determination of the total acid number (TAN) in petroleum products. The new method uses automated thermometric titration and is a superior alternative to current manual and potentiometric methods...
Read MoreCellular Dopamine and Intracellular Calcium Signaling Using the Next Generation HTS Microplate ReaderApr 6, 2011Two cell-based signaling assays from Invitrogen, Fluo-4 Direct™ Calcium Assay and Tango™-bla U2OS GPCR Assay, were performed on the PHERAstar FS HTS instrument. The PHERAstar FS has several unique features that enhance the performance of these assays including direct optic bottom reading, high resolution cell layer scanning, injection at the point of measurement and dual emission detection...
Read MoreA Fast and Simple Method For Measuring P-Glycoprotein (Pgp) InhibitionApr 4, 2011A novel assay specific for measuring inhibition of the P-glycoprotein multidrug transporter (PGp) is presented. The combination of the fast and simple fluorescence assay from the Fluorosome® company and the FLUOstar Omega microplate reader from BMG LABTECH is an ideal platform to screen for Pgp inhibitors in 96-well or 384-well format. Fluorescence kinetic signal curves and representative IC50 values for different test compounds are shown.
Read MoreMorphologi G3 with Raman microprobe helps identify particulate contaminantsMar 29, 2011A new application note describing ‘Raman chemical identification of contaminant particles’ is now available on the Malvern Instruments website. It highlights the ability of the company’s Morphologi G3 equipped with Raman microprobe accessory to target particulate impurities, an area of considerable interest to manufacturers of complex, high value products where control of particle size and shape is crucial...
Read MoreCellular Dynamics International Scientists Report on Efficient Method to Produce Footprint-Free Induced Pluripotent Stem Cells (iPSCs) Under Feeder-free ConditionsMar 25, 2011Cellular Dynamics International (CDI), the world's largest producer of human tissue cells for drug discovery and safety testing, today announced the publication of research demonstrating an efficient and scalable method of creating DNA integration-free, or footprint-free, induced pluripotent stem cells (iPSCs) under a feeder-free condition using chemically defined media...
Read MoreIdeal Injection System for Stereotaxic Mounted InjectionsMar 23, 2011The NEW PHD ULTRATM Syringe Pump drug and cell delivery system from Harvard Apparatus has been optimized to provide highly accurate and precise injections of low nanoliter to microliter volumes of cells and drugs that must be delivered to specific spatial locations...
Read MoreNew publication describes laser diffraction and automated imaging for characterizing nasal spraysMar 11, 2011A newly published article demonstrates the effectiveness of combining laser diffraction and automated particle imaging (including Raman spectroscopy) in the characterization of nasal spray products. Co-authored by materials characterization specialists from Malvern Instruments together with Julie Suman, President of Nextbreath LLC, ‘Characterizing a nasal spray formulation from droplet to API particle size' can be downloaded from the Malvern website...
Read MoreTAP Launches New Micro Bioreactor System featuring Vi-CELLMar 1, 2011The Automation Partnership (TAP), a world leader in the design and development of innovative automation for life science applications, is delighted to announce its new micro bioreactor system (ambrTM) is now available with integrated Beckman Coulter Vi-CELL® Cell Viability Analyser.
Read MoreMethod development for laser diffraction particle size analysisFeb 18, 2011Recently published and now available for download on the Malvern Instruments website, ‘Method development for laser diffraction particle size analysis' by diffraction specialist Dr Anne Virden provides practical guidance on developing robust analytical methods.
Read MoreEye-opening review of particle size reference standards enthuses USP workshop participantsFeb 11, 2011A paper reviewing the development of reference standards over three decades, given at last December's USP Workshop on Particle Size in Rockville MD, received a warm welcome from delegates. Presenter Dr Graham Rideal of Whitehouse Scientific was delighted with the level of interest his talk elicited at the event, which has translated into continuing requests for the paper itself.
Read MoreAncient Practice of Clay Eating Putting Pregnant Women at RiskFeb 7, 2011Pregnant women eating baked clay are at risk of exposing themselves and their unborn child to toxic chemicals, according to new research by De Montfort University Leicester (DMU). The practice of eating clay, known as geophagy, is an ancient tradition that is still widespread in many parts of the world, particularly Africa and parts of Asia, such as India and Bangladesh
Read MoreIDT offers oligonucleotides to suit all applicationsFeb 3, 2011Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, offers a comprehensive oligo manufacturing service with rapid turnaround times. All oligos are provided deprotected, desalted, quantified using UV spectrophotometry and checked for quality using mass spectrometry as standard, to provide the highest quality and yield accuracy currently available.
Read MoreEasing the transition into profitable manufactureFeb 1, 2011Malvern Instruments latest INFORM white paper - ‘The benefits of real-time analysis for process development' - presents the case for investing in real-time process measurement at the pilot studies stage, and is now available for free download from the company's website
Read MoreAnd the winner isJan 31, 2011Materials characterization company Malvern Instruments is known for the breadth and accessibility of the educational offering on its website, from simple news stories to in depth applications notes and interactive presentations. However, by far the most popular document is ‘Basic Principles of Particle Size Analysis', a paper written by Applications Manager Dr Alan Rawle, that has been downloaded more than 7500 times in the past three years.
Read MoreIntrinsically Safe Flame SterilizationJan 26, 2011Flame sterilization is a routine task carried out in many microbiological, clinical and life science laboratories. A new white paper from INTEGRA describes the security features of modern safety Bunsen burners in relation to the inherent dangers of flame sterilization using alcohol burners, gas cookers or a traditional Bunsen burner connected to a gas distribution system
Read More
A white paper written by Exeter Analytical describes how CHN microanalysis provides a powerful and straightforward method for determining whether a sample is pure - a key aspect of sample characterisation. The paper discuss how by providing a precise and accurate analysis of the percentage Carbon, Hydrogen and Nitrogen in an organic sample - CHN microanalysis can compliment techniques such as Mass Spectrometry (MS) and Nuclear Magnetic Resonance (NMR) Spectrometry, thus enhancing the reliability of your analytical results...
Fluidigm Europe has announced a new technical report that describes experimentation on a normal human genomic DNA sample using a prototype 200k Digital Array Chip on a modified BioMarkTM System.
A Schistosoma viability assay (96 and 384 well format) is introduced that utilizes two fluorophores. Fluorescein diacetate emission is related to living cells while propidium iodide emission indicates the number of dead cells. This assay abandons low throughput microscopy and was performed in bottom reading mode on the POLARstar Omega microplate reader from BMG LABTECH...
Traditional ELISA assays are known for their several wash steps in order to remove unbound antigen from the well. Here we introduce a fluorescence based ELISA assay that only needs one wash step. This technology is called ELISAONE™ and was developed by TGR BioSciences. The ELISAONE™ assay is both robust and sensitive as evidenced by EGF, TNFa and IL-2 data obtained with the POLARstar Omega microplate reader from BMG LABTECH...
Determining trace levels of uranium in drinking and mineral water by inductively coupled plasma mass spectroscopy (ICP-MS) requires expensive analytical instrumentation. In contrast, adsorptive cathodic stripping voltammetry (AdCSV) using Metrohm’s 797 VA Computrace is a straightforward, much less expensive alternative allowing measurements with superior sensitivity...
The efficiency of antibody molecules is dependent on their stability. Stress can cause denaturation and aggregation resulting in inactive proteins. We introduce an assay setup where stability of antibodies is measured. Shaking stress is initiated by the FLUOstar Omega microplate reader and aggregation propensity of antibody candidates is measured with the help of a fluorescence based assay in 96-well format...
GPCRs are a large family of receptors which have a broad involvement in cellular responses affecting many important body functions. An approach to measure Gi and Gq coupled receptors is the utilization of the ERK phosphorylation cascade. In this application note the POLARstar Omega microplate reader from BMG LABTECH is used to detect ERK phosphorylation using an AlphaScreen® Surefire® proximity-based assay in 384-well format...
Two cell-based signaling assays from Invitrogen, Fluo-4 Direct™ Calcium Assay and Tango™-bla U2OS GPCR Assay, were performed on the PHERAstar FS HTS instrument. The PHERAstar FS has several unique features that enhance the performance of these assays including direct optic bottom reading, high resolution cell layer scanning, injection at the point of measurement and dual emission detection...
A novel assay specific for measuring inhibition of the P-glycoprotein multidrug transporter (PGp) is presented. The combination of the fast and simple fluorescence assay from the Fluorosome® company and the FLUOstar Omega microplate reader from BMG LABTECH is an ideal platform to screen for Pgp inhibitors in 96-well or 384-well format. Fluorescence kinetic signal curves and representative IC50 values for different test compounds are shown.
A new application note describing ‘Raman chemical identification of contaminant particles’ is now available on the Malvern Instruments website. It highlights the ability of the company’s Morphologi G3 equipped with Raman microprobe accessory to target particulate impurities, an area of considerable interest to manufacturers of complex, high value products where control of particle size and shape is crucial...
Cellular Dynamics International (CDI), the world's largest producer of human tissue cells for drug discovery and safety testing, today announced the publication of research demonstrating an efficient and scalable method of creating DNA integration-free, or footprint-free, induced pluripotent stem cells (iPSCs) under a feeder-free condition using chemically defined media...
The NEW PHD ULTRATM Syringe Pump drug and cell delivery system from Harvard Apparatus has been optimized to provide highly accurate and precise injections of low nanoliter to microliter volumes of cells and drugs that must be delivered to specific spatial locations...
A newly published article demonstrates the effectiveness of combining laser diffraction and automated particle imaging (including Raman spectroscopy) in the characterization of nasal spray products. Co-authored by materials characterization specialists from Malvern Instruments together with Julie Suman, President of Nextbreath LLC, ‘Characterizing a nasal spray formulation from droplet to API particle size' can be downloaded from the Malvern website...
The Automation Partnership (TAP), a world leader in the design and development of innovative automation for life science applications, is delighted to announce its new micro bioreactor system (ambrTM) is now available with integrated Beckman Coulter Vi-CELL® Cell Viability Analyser.
Recently published and now available for download on the Malvern Instruments website, ‘Method development for laser diffraction particle size analysis' by diffraction specialist Dr Anne Virden provides practical guidance on developing robust analytical methods.
A paper reviewing the development of reference standards over three decades, given at last December's USP Workshop on Particle Size in Rockville MD, received a warm welcome from delegates. Presenter Dr Graham Rideal of Whitehouse Scientific was delighted with the level of interest his talk elicited at the event, which has translated into continuing requests for the paper itself.
Pregnant women eating baked clay are at risk of exposing themselves and their unborn child to toxic chemicals, according to new research by De Montfort University Leicester (DMU). The practice of eating clay, known as geophagy, is an ancient tradition that is still widespread in many parts of the world, particularly Africa and parts of Asia, such as India and Bangladesh
Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, offers a comprehensive oligo manufacturing service with rapid turnaround times. All oligos are provided deprotected, desalted, quantified using UV spectrophotometry and checked for quality using mass spectrometry as standard, to provide the highest quality and yield accuracy currently available.
Malvern Instruments latest INFORM white paper - ‘The benefits of real-time analysis for process development' - presents the case for investing in real-time process measurement at the pilot studies stage, and is now available for free download from the company's website
Materials characterization company Malvern Instruments is known for the breadth and accessibility of the educational offering on its website, from simple news stories to in depth applications notes and interactive presentations. However, by far the most popular document is ‘Basic Principles of Particle Size Analysis', a paper written by Applications Manager Dr Alan Rawle, that has been downloaded more than 7500 times in the past three years.
Flame sterilization is a routine task carried out in many microbiological, clinical and life science laboratories. A new white paper from INTEGRA describes the security features of modern safety Bunsen burners in relation to the inherent dangers of flame sterilization using alcohol burners, gas cookers or a traditional Bunsen burner connected to a gas distribution system