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New microfluidic system enhances protein analysis workflows for the study of weak protein-protein interactions by mass photometry

Refeyn®, a leader in mass photometry technology, has unveiled its latest innovation, the MassFluidix® High Concentration (HC) microfluidic system, which extends the analytical strength of mass photometry to high-concentration protein samples. Compatible with Refeyn’s  TwoMP mass photometers, the MassFluidix HC enables accurate measurement of low-affinity biomolecular interactions through rapid dilution, such that the interaction state at micromolar concentrations is captured before their equilibrium is disrupted. Researchers can now analyze samples with concentrations of up to 90 µM, a 100-fold increase on previous capabilities. This approach builds on the speed and simplicity already offered by mass photometry, and opens up new applications, such as the study of low-affinity protein-protein interactions, transient binding, and oligomerization, all label-free, in solution, and at the single-molecule level.

Designed for easy and intuitive operation, the MassFluidix HC system provides precise and rapid dilution of up to 10,000-fold in milliseconds, facilitating the detection of low-affinity complexes that have not previously been observed. The system consists of two parts: the microfluidics device and a consumable chip for immediate analysis allowing the sample to reach the observation window within <50 ms of the beginning of the dilution process.

The introduction of Refeyn’s MassFluidix HC system further highlights the company's commitment to meeting the needs of its customers and unique challenges of protein research by expanding the capabilities of mass photometry. Commercialized by Refeyn, mass photometry offers a fast, cost-effective, and precise approach to measuring molecular mass distribution at the single-molecule level, providing researchers with a powerful tool for understanding complex biological systems.  For researchers looking to expand the potential of mass photometry and streamline their protein analysis workflows, Refeyn’s MassFluidix HC system is an essential addition to their laboratory.

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About Refeyn

Refeyn Ltd. specializes in the development, production, and distribution of mass photometry technology for industry and academia. Mass photometry is a unique bioanalytical technology pioneered by Refeyn that enables the measurement of molecular mass at the single-particle level by quantifying light scattering from biomolecules in solution. Spun out of Oxford University in 2018, the company aims to make mass photometry accessible to all in the scientific community, enabling users to achieve new and better outcomes. Refeyn has been recognized for its unique approach to biomolecular analysis through multiple awards, including the Royal Society of Chemistry Emerging Technologies Competition. Applications of mass photometry include: sample characterization and analysis of protein oligomerization, biomolecular interactions, and macromolecular assemblies. Utilizing the novel technology of mass photometry, Refeyn’s products are capable of accurate mass measurement of single molecules in solution, in their native state, and without the need for labels, opening up new possibilities for bioanalytic and research into biomolecular function.