Channels

 

Special Offers & Promotions

 

 

Latest News

 

 

View Channel

New Products

 

 

View Channel

Video Presentations

 

 

View Channel

Separation Science

 

 

View Channel

Microscopy & Image Analysis

 

 

View Channel

Laboratory Automation & IT Solutions

 

 

View Channel

 

New Super-Resolution System for 3D Localization Microscopy

Leica SR GSD 3D - Highly Precise and Reproducible Localization of Single Molecules and Cellular Structures in Three Dimensions 

Leica SR GSD 3D - new super-resolution system for 3D localization microscopySuper-resolution is a major trend in light microscopy. Extending its portfolio of super-resolution microscopy products, Leica has now launched the Leica SR GSD 3D, a widefield system that not only offers 2D, but now also 3D super-resolution imaging of molecules and cellular structures. Based on GSD (Ground State Depletion) or dSTORM technology, the widefield fluorescence microscope attains resolutions of down to 20 nm in the lateral and 50 nm in the axial direction. The Leica SR GSD 3D also scores with its unique precision for localizing single molecules, its system stability and its optical performance. These attributes are essential for reproducible, high-quality results in a very short time.  The benefits of the Leica SR GSD 3D are rounded off by the easy operation of the fully automated system and software. 

The Leica SR GSD 3D is equipped with a 160x high-performance objective specifically developed for super-resolution microscopy. Its design is optimized for high-power laser emissions. The extremely low autofluorescence ensures a high signal-to-noise ratio, which is ideal for single molecule detection applications. To enable the localization of molecules in z direction, i.e. above and below the focal plane, the Leica SR GSD 3D uses the astigmatism effect. Astigmatism is actually an image aberration, but is created deliberately here with a cylindrical lens. Corresponding images allow determination of the z position of a detected molecule, thereby enabling 3D reconstruction. To ensure reproducibility of results, the cylindrical lens is precisely positioned into the beam path as soon as the system is switched from 2D to 3D recording - not by hand, but automatically by a single mouse click through software control.  

The Leica SR GSD 3D has been optimized in several ways for reliable localization results. All the optical components are apochromatically corrected to high Leica Microsystems standards. Building on this, the software enables precise calibration of the system for the localization of molecules in x, y and z. Besides the standard calibration with gold beads, color-specific calibration with fluorescence beads or dye molecules of the specimen is available. The special SuMo technology (SUpressed MOtion) ensures minimum drift and maximum stability during detection. 

"3D visualization of cellular structures with nanometer resolution allows scientists in biomedical research to gain new insights," explains Sebastian Tille, Director Widefield Imaging  at Leica Microsystems. "The new Leica SR GSD 3D is an excellent tool for gaining a better understanding of molecular structures or cellular protein transport processes, for example. Experts are already confirming the outstanding performance and reliability of the new system after first tests." 

The Leica SR GSD 3D is based on a fully automated TIRF system (Total Internal Reflection Fluo-rescence), allowing super-resolution to be combined with TIRF microscopy. The system can also be used for a wide variety of applications in all fields of live cell or advanced fluorescence microscopy. As a multi-functional system, the Leica SR GSD 3D gives researchers the freedom to tailor the system exactly to their needs. Another key advantage of GSD technology is that it can be used with standard fluorescence labels. The Leica SR GSD 3D is the successor to the Leica SR GSD, which was recognized by international expert commitees many times, such as with the prestigious R&D100 award.


more about Lecia SR GSD 3D


more about Leica


more news from Leica


 


If you have not logged into the website then please enter your details below.



 

News Channels

 

 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month

Top 10 most popular articles this month

 

 

Today's Picks

 

 

 

 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


 

Media Partners

 

Exhibitions & Events