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New Leica DFC365 FX Fluorescence Camera for Live Cell Imaging

The Leica DFC365 FX To successfully document live cells and rapidly fading fluorescence specimens, a camera technology particularly designed for the purpose is indispensable. The new Leica DFC365 FX digital microscope camera from Leica Microsystems combines exceptional image quality with very high temporal resolution for rapid time-lapse recordings. The Leica DFC365 FX is setting new standards in its class enabling researchers to work efficiently, even with weakly fluorescing specimens. Equipped with a highly sensitive CCD sensor (pixel size 6.45 µm) and active Peltier cooling, it is ideal for a wide range of applications - from basic fluorescence documentation to demanding experiments such as TIRF, FRET or structured illumination.

The Leica DFC365 FX achieves hitherto unattainable acquisition rates of 21 fps at full resolution. In "Overlapping Mode", an image can be captured while the previous image is still being read out. Data are transferred rapidly to the PC via a FireWire-B interface. Besides high-speed image acquisition at 40 MHz, the pixel clocking rate of the sensor can also be set to 20 MHz or 1.6 MHz as required. This yields brilliant fluorescence images with a superb signal-to-noise ratio.

The optional NIR (Near Infra-Red) Mode extends the operating range of the camera for fluorescence markers emitting in the wavelength range above 700 nm, which are difficult to capture with conventional CCD technology.

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